公开(公告)号：US07371926B2

公开(公告)日：2008-05-13

申请号：US11399675

申请日：2006-04-06

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： C12N15/31 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

公开(公告)号：US07049485B2

公开(公告)日：2006-05-23

申请号：US09981900

申请日：2001-10-18

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： C12N15/31 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

公开(公告)号：US07569745B2

公开(公告)日：2009-08-04

申请号：US12151270

申请日：2008-05-05

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： C12N31/82 ,  C12N52/82 ,  C12N82/82 ,  A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

公开(公告)号：US07696411B2

公开(公告)日：2010-04-13

申请号：US11986073

申请日：2007-11-20

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： C12N15/31 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

摘要翻译： 本发明提供了转基因植物，其在收获后将其中的木质素和纤维素降解成可进一步发酵成乙醇或其它产物的可发酵糖。 特别地，转基因植物包含来自微生物的木质素酶和纤维素酶基因，其可操作地连接到编码信号肽的DNA，所述信号肽将由其产生的融合多肽靶向植物的细胞器，特别是叶绿体。 当收获转基因植物时，研磨植物以释放木质素酶和纤维素酶，然后使转基因植物的木质素和纤维素降解以产生可发酵糖。

公开(公告)号：US20080235830A1

公开(公告)日：2008-09-25

申请号：US12151270

申请日：2008-05-05

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.