公开(公告)号：US08673031B2

公开(公告)日：2014-03-18

申请号：US13202011

申请日：2010-08-24

申请人： Bruce E. Dale ,  Bryan Ritchie ,  Derek Marshall

发明人： Bruce E. Dale ,  Bryan Ritchie ,  Derek Marshall

IPC分类号： C10L5/40 ,  C10L5/06 ,  C10L5/00

CPC分类号： D21B1/021 ,  A23K10/37 ,  C10L5/363 ,  C10L5/44 ,  Y02E50/10 ,  Y02E50/30 ,  Y02P60/877 ,  Y10T428/2938

摘要： A product comprising at least one densified biomass particulate of a given mass having no added binder and comprised of a plurality of lignin-coated plant biomass fibers is provided, wherein the at least one densified biomass particulate has an intrinsic density substantially equivalent to a binder-containing densified biomass particulate of the same given mass and h a substantially smooth, non-flakey outer surface. Methods for using and making the product are also described.

摘要翻译： 提供了包含至少一种具有不含添加的粘合剂并由多种木质素涂覆的植物生物质纤维组成的给定质量的致密化生物质颗粒的产品，其中所述至少一种致密化的生物质颗粒具有基本上等同于粘合剂 - 含有相同质量的致密化生物质颗粒和基本平滑的非鳞片状外表面。 还描述了使用和制造产品的方法。

公开(公告)号：US08394611B2

公开(公告)日：2013-03-12

申请号：US12226763

申请日：2007-04-30

申请人： Bruce E. Dale ,  Lee R. Lynd ,  Mark Laser

发明人： Bruce E. Dale ,  Lee R. Lynd ,  Mark Laser

IPC分类号： C12P19/02 ,  C12P7/02 ,  C12P19/00 ,  C13K1/02 ,  C01C1/00

CPC分类号： C12P19/02 ,  C12P7/10 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  Y02E50/16

摘要： A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

摘要翻译： 描述了使用具有或不具有无水氨添加的浓氢氧化铵来处理生物质以使结构碳水化合物更易于和/或消化的方法。 该方法优选使用蒸汽从生物质中除去氨以进行再循环。 来自结构碳水化合物的单糖的方法产率是好的，特别是通过结构碳水化合物的酶水解测量。 单糖用作乙醇生产的动物饲料和能源。

公开(公告)号：US07696411B2

公开(公告)日：2010-04-13

申请号：US11986073

申请日：2007-11-20

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： C12N15/31 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

摘要翻译： 本发明提供了转基因植物，其在收获后将其中的木质素和纤维素降解成可进一步发酵成乙醇或其它产物的可发酵糖。 特别地，转基因植物包含来自微生物的木质素酶和纤维素酶基因，其可操作地连接到编码信号肽的DNA，所述信号肽将由其产生的融合多肽靶向植物的细胞器，特别是叶绿体。 当收获转基因植物时，研磨植物以释放木质素酶和纤维素酶，然后使转基因植物的木质素和纤维素降解以产生可发酵糖。

公开(公告)号：US20080235830A1

公开(公告)日：2008-09-25

申请号：US12151270

申请日：2008-05-05

申请人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

发明人： Masomeh B. Sticklen ,  Bruce E. Dale ,  Shahina B. Maqbool

IPC分类号： A01H5/00

CPC分类号： C12N9/2437 ,  C12N9/2445 ,  C12N15/8207 ,  C12N15/8242 ,  C12N15/8246 ,  C12N15/8255 ,  C12N15/8257 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01004 ,  C12Y302/01011 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

公开(公告)号：US09644222B2

公开(公告)日：2017-05-09

申请号：US13997043

申请日：2011-12-22

申请人： Venkatesh Balan ,  Bruce E Dale ,  Shishir Chundawat ,  Leonardo Sousa

发明人： Venkatesh Balan ,  Bruce E Dale ,  Shishir Chundawat ,  Leonardo Sousa

IPC分类号： C12P19/14 ,  C12P19/02 ,  C08B1/00 ,  C12P7/10

CPC分类号： C12P19/14 ,  C08B1/003 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  Y02E50/16 ,  Y02P30/20

摘要： A method for pretreating biomass is provided, which includes, in a reactor, allowing gaseous ammonia to condense on the biomass and react with water present in the biomass to produce pretreated biomass, wherein reactivity of polysaccharides in the biomass is increased during subsequent biological conversion as compared to the reactivity of polysaccharides in biomass which has not been pretreated. A method for pretreating biomass with a liquid ammonia and recovering the liquid ammonia is also provided. Related systems which include a biochemical or biofuel production facility are also disclosed.

公开(公告)号：US08968515B2

公开(公告)日：2015-03-03

申请号：US12976344

申请日：2010-12-22

申请人： Venkatesh Balan ,  Bruce E. Dale ,  Shishir Chundawat ,  Leonardo Sousa

发明人： Venkatesh Balan ,  Bruce E. Dale ,  Shishir Chundawat ,  Leonardo Sousa

IPC分类号： D21C11/00 ,  D21C9/10 ,  D21C3/22 ,  C13K1/02 ,  B01J3/00 ,  C01C1/00 ,  C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C08B1/00

CPC分类号： C12P19/14 ,  C08B1/003 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  Y02E50/16 ,  Y02P30/20

摘要： A method of alkaline pretreatment of biomass, in particular, pretreating biomass with gaseous ammonia.

摘要翻译： 一种碱性预处理生物质的方法，特别是用气态氨预处理生物质。

公开(公告)号：US20120325202A1

公开(公告)日：2012-12-27

申请号：US13591092

申请日：2012-08-21

申请人： Bruce E. Dale

发明人： Bruce E. Dale

IPC分类号： C08B1/00

CPC分类号： C12P19/02 ,  C12P7/10 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  Y02E50/16

摘要： A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

摘要翻译： 描述了使用具有或不具有无水氨添加的浓氢氧化铵来处理生物质以使结构碳水化合物更易于和/或消化的方法。 该方法优选使用蒸汽从生物质中除去氨以进行再循环。 来自结构碳水化合物的单糖的方法产率是好的，特别是通过结构碳水化合物的酶水解测量。 单糖用作乙醇生产的动物饲料和能源。

公开(公告)号：US20090221042A1

公开(公告)日：2009-09-03

申请号：US12226763

申请日：2007-04-30

申请人： Bruce E. Dale ,  Lee R. Lynd ,  Mark Laser

发明人： Bruce E. Dale ,  Lee R. Lynd ,  Mark Laser

IPC分类号： C12P19/02 ,  C13K1/02 ,  C12P19/00 ,  C12P7/02 ,  C01C1/00 ,  B01J19/00

CPC分类号： C12P19/02 ,  C12P7/10 ,  C12P2201/00 ,  C13K1/02 ,  C13K13/002 ,  Y02E50/16

摘要： A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.

摘要翻译： 描述了使用具有或不具有无水氨添加的浓氢氧化铵来处理生物质以使结构碳水化合物更易于和/或消化的方法。 该方法优选使用蒸汽从生物质中除去氨以进行再循环。 来自结构碳水化合物的单糖的方法产率是好的，特别是通过结构碳水化合物的酶水解测量。 单糖用作乙醇生产的动物饲料和能源。

公开(公告)号：US20090042259A1

公开(公告)日：2009-02-12

申请号：US12221972

申请日：2008-08-08

申请人： Bruce E. Dale ,  Farzaneh Teymouri ,  Shishir Chundawat ,  Venkatesh Balan

发明人： Bruce E. Dale ,  Farzaneh Teymouri ,  Shishir Chundawat ,  Venkatesh Balan

IPC分类号： C12P19/02

CPC分类号： C12P19/02 ,  C12P19/14

摘要： The present invention describes a process for at least a 90% conversion of a plant biomass preferably by a reduction of the units of cellulase needed and by using a xylanase which acts synergistically with the cellulase to improve the yield of xylose and glucose as sugars. The process enables greater conversion of a lignocellulosic plant biomass to glucose and xylose for use as animal feeds and as fermentation as medium for producing ethanol.

摘要翻译： 本发明描述了一种用于植物生物质的至少90％转化的方法，优选通过减少所需的纤维素酶单位和通过使用与纤维素酶协同作用的木聚糖酶来提高作为糖的木糖和葡萄糖的产率。 该方法使得木质纤维素植物生物质能够更好地转化成葡萄糖和木糖用作动物饲料，并且作为用于生产乙醇的培养基的发酵。

公开(公告)号：US6106888A

公开(公告)日：2000-08-22

申请号：US232980

申请日：1999-01-19

申请人： Bruce E. Dale ,  Justin K. Weaver

发明人： Bruce E. Dale ,  Justin K. Weaver

IPC分类号： C08H8/00 ,  A23K1/00 ,  A23K1/12 ,  A23K1/22 ,  A23L1/00 ,  A23L1/10 ,  A23P1/00 ,  A23P1/12 ,  A23P1/14 ,  B29C47/00 ,  B29C47/60 ,  C08B1/00 ,  C08B37/00 ,  C12S3/04 ,  A23K3/00

CPC分类号： B29C47/0825 ,  A23K10/32 ,  A23K40/20 ,  A23K50/15 ,  A23P30/34 ,  B29C47/0004 ,  B29C47/0023 ,  B29C47/003 ,  B29C47/60 ,  B29K2093/00 ,  C12P2201/00 ,  Y10S426/807

摘要： A process and screw in barrel apparatus (10) for expanding cellulosic materials is described. The expanded cellulosic material is useful as an animal feed and a nutrient source for fermentation processes.

摘要翻译： 描述了用于膨胀纤维素材料的桶装置（10）中的工艺和螺杆。 膨胀的纤维素材料可用作动物饲料和用于发酵过程的营养源。