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公开(公告)号:US20120231533A1
公开(公告)日:2012-09-13
申请号:US13126700
申请日:2009-10-28
申请人: Mark R. Holl , Deirdre R. Meldrum , A. Cody Youngbull , Haixin Zhu , Jeff Houkal , Yanqing Tian , Shashanka Ashili , Laimonas Kelbauskas , Roger Johnson , Shih-hui Chao , Peter Wiktor , Alex Jen , Lloyd Burgess , Sarah McQuaid , Ai Brunner , Peter Kahn
发明人: Mark R. Holl , Deirdre R. Meldrum , A. Cody Youngbull , Haixin Zhu , Jeff Houkal , Yanqing Tian , Shashanka Ashili , Laimonas Kelbauskas , Roger Johnson , Shih-hui Chao , Peter Wiktor , Alex Jen , Lloyd Burgess , Sarah McQuaid , Ai Brunner , Peter Kahn
IPC分类号: C12M1/34
摘要: A chamber device for analyzing living cell(s). The chamber device includes a base and a lid that when reversibly pressed closed create a chamber. The base is configured with an optically transparent well to contain at least one cell. The lid has a breadth greater than the base and is configured to contain at least one sensor. The lid is further configured with a lip that when pressed between the lid and the base creates an impermeable seal. The base and the lid are configured so that, when closed and in use, the sensor remains spatially apart from the at least one cell.
摘要翻译: 用于分析活细胞的室装置。 腔室装置包括底座和盖子,当可逆地按压时,盖子产生一个腔室。 基底配置有光学透明的孔以容纳至少一个细胞。 盖子的宽度大于基座,并被配置成包含至少一个传感器。 盖子进一步配置有唇部,当在盖子和基座之间被按压时,唇部产生不可渗透的密封。 底座和盖被构造成使得当关闭并且在使用中时,传感器在空间上保持与至少一个单元隔开。
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公开(公告)号:US10260090B2
公开(公告)日:2019-04-16
申请号:US15213194
申请日:2016-07-18
IPC分类号: C12P19/34 , C12Q1/68 , C12Q1/6844
摘要: A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.
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公开(公告)号:US20170107549A1
公开(公告)日:2017-04-20
申请号:US15213194
申请日:2016-07-18
IPC分类号: C12P19/34
CPC分类号: C12Q1/6844 , C12Q2525/161
摘要: A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.
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