Abstract:
This disclosure describes a cell genetically modified to detect ribosome inhibition in the cell and methods involving such a cell. Generally, the genetically-modified cell includes an aminoglycoside-sensitive orthogonal 16S rRNA (O-16S) coding region bearing a mutated anti-Shine-Dalgarno (O-ASD) sequence, a repressor/operator system, and a polynucleotide encoding a detectable reporter under transcriptional control of the repressor/operator system. The repressor/operator system includes a coding region that encodes a transcriptional regulator and having an orthogonal SD (O-SD) sequence complementary to the 16S rRNA O-ASD sequence. The operator sequence, which is repressable by the transcriptional regulator, is operably linked to the polynucleotide encoding a detectable reporter.
Abstract:
An entertainment console installed in a vehicle includes a docking bay for docking portable entertainment devices, such as portable media players (PMPs). A base of the console may be attached to the passenger compartment ceiling, with a display that can be flipped down from the base. A docked PMP can be played through the console. Video may be rendered on the console screen, while audio may be played through the console speaker(s), headphone jacks available on the console, or the speakers of the vehicle's audio system. Power for the PMP's operation is provided by the vehicle's electrical system. The console may also include additional docking bay(s), enabling simultaneous docking of multiple PMPs that may be played at the same time. A presentation from one PMP (e.g., a DVD player) can also be recorded on another PMP (e.g., a hard drive-based player). The screen may rotate around the vertical axis.