公开(公告)号：US12091718B2

公开(公告)日：2024-09-17

申请号：US18049805

申请日：2022-10-26

申请人： North Carolina State University

发明人： Gavin J. Williams ,  Christian Kasey ,  Yiwei Li

IPC分类号： C12Q1/6897 ,  C07K14/245 ,  C12N15/09

CPC分类号： C12Q1/6897 ,  C07K14/245 ,  C12N15/09

摘要： The present disclosure relates to high-throughput detection of polyketides using genetically encoded biosensors.

公开(公告)号：US12065642B2

公开(公告)日：2024-08-20

申请号：US16793272

申请日：2020-02-18

申请人： Sigma-Aldrich Co. LLC

发明人： Fuqiang Chen ,  Xiao Ding ,  Yongmei Feng ,  Gregory Davis

IPC分类号： C12N9/22 ,  C12N15/10 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90 ,  C12Q1/6897 ,  A61K48/00

CPC分类号： C12N15/102 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/907 ,  C12Q1/6897 ,  A61K48/005 ,  C07K2319/09 ,  C07K2319/10 ,  C07K2319/80 ,  C07K2319/81 ,  C12N2310/20 ,  C12N2800/80

摘要： Compositions and methods for using nucleosome interacting protein domains to increase accessibility of programmable DNA modification proteins to target chromosomal sequences, thereby increasing efficiency of targeted genome/epigenetic modification in eukaryotic cells.

公开(公告)号：US12037707B2

公开(公告)日：2024-07-16

申请号：US17045120

申请日：2019-04-05

申请人： Massachusetts Eye and Ear Infirmary ,  The Schepens Eye Research Institute, Inc.

发明人： Luk H. Vandenberghe ,  Eric Zinn ,  Heikki Turunen

IPC分类号： C40B50/06 ,  C12N15/10 ,  C12Q1/6897 ,  C40B40/08

CPC分类号： C40B50/06 ,  C12N15/1093 ,  C12Q1/6897 ,  C40B40/08

摘要： This disclosure describes compositions, methods, and systems for constructing defined variation in a contiguous functional genetic unit in association with a unique sequence identifier (“a barcode”) in a combinatorial manner.

公开(公告)号：US12030046B2

公开(公告)日：2024-07-09

申请号：US16880754

申请日：2020-05-21

申请人： CITROGENE INC.

发明人： S Abbas Hosseini ,  Amir Tork

IPC分类号： C12Q1/6888 ,  B01L3/00 ,  C12N1/06 ,  C12Q1/44 ,  C12Q1/6897 ,  G01N21/64

CPC分类号： B01L3/502715 ,  B01L3/502738 ,  C12N1/06 ,  C12Q1/44 ,  C12Q1/6888 ,  C12Q1/6897 ,  G01N21/6428 ,  B01L2200/16 ,  B01L2300/047 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/168 ,  B01L2300/1861 ,  B01L2400/06 ,  G01N2021/6439

摘要： An apparatus and method is disclosed for rapid identification of a microorganism within sampling device. The sampling device has a plurality of reaction chambers each having a different reactive agent for reacting with the microorganism to indicate the presence of a microorganism in said reaction chamber. A detector detects each of the plurality of reaction chamber for detecting the presence of a microorganism in said reaction chamber. The invention automates CRISPR CAS 12 and/or CAS 13 method. The invention is a general platform for detection of segments of DNA or RNA using CRISPR CAS 12 and/or CAS 13 proteins.

公开(公告)号：US20240141445A1

公开(公告)日：2024-05-02

申请号：US17778927

申请日：2020-11-16

申请人： CeMM - Forschungszentrum für Molekulare Medizin GmbH

发明人： Stefan KUBICEK ,  Andreas REICHER

IPC分类号： C12Q1/6897 ,  C12N15/10 ,  G01N33/50

CPC分类号： C12Q1/6897 ,  C12N15/1086 ,  G01N33/5038 ,  G01N2500/10

摘要： The present invention relates to a method for monitoring the effect of an environmental factor on the proteome of a cell. Furthermore, cell populations are provided that comprise multiple cells each comprising an inserted tag sequence in an intron of said cell, wherein the tag is inserted in-frame with the preceding exonic sequence and wherein the intron into which the tag is inserted is different between cells.

公开(公告)号：US20240110231A1

公开(公告)日：2024-04-04

申请号：US18242217

申请日：2023-09-05

申请人： Albert Einstein College of Medicine

发明人： Robert H. Singer ,  Evelina Tutucci ,  Maria Vera Ugalde

IPC分类号： C12Q1/6841 ,  C12Q1/6897

CPC分类号： C12Q1/6841 ,  C12Q1/6897

摘要： An RNA tagging system for visualization of single mRNA molecules based on a MSB-MCP system, as well as methods of use.

公开(公告)号：US20240093316A1

公开(公告)日：2024-03-21

申请号：US18520007

申请日：2023-11-27

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Guochun Gong ,  Charleen Hunt ,  Daisuke Kajimura ,  Suzanne Hartford ,  Brian Zambrowicz

IPC分类号： C12Q1/6897 ,  A01K67/027 ,  A61K9/00 ,  A61K49/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC分类号： C12Q1/6897 ,  A01K67/027 ,  A01K67/0275 ,  A61K9/0019 ,  A61K49/0008 ,  C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0393 ,  C07K2319/60 ,  C12N2310/20 ,  C12N2750/14141 ,  C12N2800/80 ,  C12Q2600/106

摘要： Methods and compositions are provided herein for assessing CRISPR/Cas-mediated non-homologous end joining (NHEJ) activity and/or CRISPR/Cas-induced recombination of a target genomic locus with an exogenous donor nucleic acid in vivo and ex vivo. The methods and compositions employ cells and non-human animals comprising a Cas expression cassette such as a genomically integrated Cas expression cassette so that the Cas protein can be constitutively available or available in a tissue-specific or temporal-specific manner. Methods and compositions are also provided for making and using these non-human animals, including use of these non-human animals to assess CRISPR/Cas activity in vivo via adeno-associated virus (AAV)-mediated delivery of guide RNAs to the non-human animals.

公开(公告)号：US11926841B2

公开(公告)日：2024-03-12

申请号：US17481576

申请日：2021-09-22

申请人： SIRION BIOTECH GmbH

发明人： Ulrich Koszinowski ,  Simona Langer ,  Zsolt Ruzsics ,  Christian Thirion

IPC分类号： C07H21/04 ,  C12N15/86 ,  C12Q1/02 ,  C12Q1/6897 ,  C12Q1/70

CPC分类号： C12N15/86 ,  C12Q1/025 ,  C12Q1/6897 ,  C12Q1/70 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2830/002

摘要： The present invention relates to a cell comprising (aa) a nucleic acid comprising in 5′ to 3′ direction (i) at least one adeno-associated virus (AAV) inverted terminal repeat (ITR) sequence; (ii) a promoter which is capable of being activated by (a) helper polypeptide(s) and optionally (a) helper polynucleotide(s); and (iii) a transgenic coding sequence under the control of said promoter of (aa)(ii); and (ab) a nucleic acid comprising in 5′ to 3′ direction (i) a promoter which is capable of being activated by said helper polypeptide(s) and optionally said helper polynucleotide(s); and (ii) at least one AAV rep gene coding sequence under the control of said promoter of (ab)(i); wherein said cell does not comprise an AAV cap gene and/or is not able to express any AAV cap gene product.

公开(公告)号：US11898157B2

公开(公告)日：2024-02-13

申请号：US16894416

申请日：2020-06-05

申请人： David Nielsen ,  Christopher Jones

发明人： David Nielsen ,  Christopher Jones

IPC分类号： C12N15/90 ,  C12Q1/6897 ,  C12N15/86 ,  C12Q1/6874

CPC分类号： C12N15/902 ,  C12N15/86 ,  C12Q1/6874 ,  C12Q1/6897

摘要： Compositions and methods for genetically modifying at least one nucleic acid sequence of interest in a polyploid organism.

公开(公告)号：US20240018607A1

公开(公告)日：2024-01-18

申请号：US18252869

申请日：2021-11-12

申请人： LogicInk Corporation

发明人： Carlos Edel OLGUIN ALVAREZ ,  Hendrik DIETZ ,  Steven WROBEL

IPC分类号： C12Q1/6897 ,  C12Q1/44

CPC分类号： C12Q1/6897 ,  C12Q1/44

摘要： Systems comprising a translation module, an amplification module comprising one or more sensing modalities, and a detection module configured to accept the amplifier output and methods of using the same are provided. The systems or methods can be capable of detecting trace amounts of nucleic acid molecules with high sensitivity and specificity in a device-free system with naked-eye readable output. The systems can comprise three layers incorporated into a lateral flow assay and/or a paper strip assay, in such systems, the first layer can comprise the translation module, the second layer can comprise the amplification module, and the third layer can comprise the detection module.