公开(公告)号：US4237230A

公开(公告)日：1980-12-02

申请号：US52444

申请日：1979-06-26

申请人： Takao Iida ,  Sho Ozaki ,  Toshihiko Kotaka

发明人： Takao Iida ,  Sho Ozaki ,  Toshihiko Kotaka

IPC分类号： A23C9/12 ,  A61K38/47 ,  C12N9/38

CPC分类号： A23C9/1206 ,  A61K38/47 ,  C12N9/2471 ,  C12N9/86 ,  C12Y302/01023 ,  C12Y305/02006 ,  Y10S435/835

摘要： A novel lactase having a molecular weight of about 3.times.10.sup.5, an optimum pH value of about 6.0, an optimum temperature of about 60.degree. C. and at least 1 of the ratio of the activity for hydrolyzing lactose to the activity for hydrolyzing a synthetic substrate: o-nitrophenyl-.beta.-D-galactopyranoside (Lact/ONPG ratio), which is produced by cultivating a microorganism of the genus Bacillus being capable of producing the enzyme, particularly Bacillus circulans LOB 377 (ATCC No. 31382) and isolating from the culture broth. This novel lactase is characteristic in the excellent thermostability and the high Lact/ONPG ratio and hence is useful for treating milk and milk products as well as for preventing diarrhea due to lactose intolerance, especially in babies and infants.

摘要翻译： 一种新型乳糖酶，其分子量约为3×10 5，最佳pH值为约6.0，最适温度为约60℃，水解乳糖的活性与水解合成底物的活性的比例为至少1： O-硝基苯基-β-D-吡喃半乳糖苷（Lact / ONPG比），其通过培养能够产生酶的芽孢杆菌属的微生物，特别是环状芽孢杆菌LOB 377（ATCC No.31382）产生并从培养物中分离 肉汤。 这种新型乳糖酶具有优异的热稳定性和高的乳酸/ ONPG比值，因此可用于处理牛奶和乳制品以及防止由于乳糖不耐受引起的腹泻，特别是在婴儿和婴儿中。