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173 条记录 (耗时 0.05 秒)

    DETECTING BETA-LACTAMASE ENZYME ACTIVITY
    1.
    发明公开
    DETECTING BETA-LACTAMASE ENZYME ACTIVITY 审中-公开

    公开(公告)号：US20240110220A1

    公开(公告)日：2024-04-04

    申请号：US18262523

    申请日：2022-01-25

    申请人： COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES ,  UNIVERSITE PARIS-SACLAY

    发明人： Herve VOLLAND ,  Christian MOGUET ,  Thierry NAAS

    IPC分类号： C12Q1/34 ,  C12Q1/18

    CPC分类号： C12Q1/34 ,  C12Q1/18 ,  C12Y305/02006

    摘要： It is essential to have efficient, simple, quick and transportable tools for reliably identifying bacteria that are multiresistant to antibiotics, more specifically extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, which are the most widespread among Enterobacteriaceae. The present invention meets this requirement through its ease of use and its speed. The invention is based on detecting the enzyme activity of β-lactam hydrolysis using an antibody capable of discriminating between the intact form of the β-lactam ring of a β-lactam and its hydrolysis product. This antibody can be used in kits and methods enabling for rapidly detecting (in less than one hour), without using expensive equipment (a small strip visible to the naked eye), the presence of bacteria producing penicillin-type, plasmid-mediated or hyper-produced AmpC enzymes, of ESBL or carbapenemase from colonies or in a sample.

    UNIVERSAL PROTEIN OVEREXPRESSION TAG COMPRISING RAMP FUNCTION, AND APPLICATION THEREOF
    9.
    发明申请
    UNIVERSAL PROTEIN OVEREXPRESSION TAG COMPRISING RAMP FUNCTION, AND APPLICATION THEREOF 审中-公开
    包含RAMP功能的通用蛋白过表达及其应用

    公开(公告)号：US20160017341A1

    公开(公告)日：2016-01-21

    申请号：US14773529

    申请日：2014-02-27

    申请人： INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY

    发明人： Geun-Joong KIM ,  Won Ji PARK ,  Sung-Hwan YOU ,  Jin-Young LEE ,  Eun-Bin LEE ,  Sa-Young MIN

    IPC分类号： C12N15/67 ,  C12P21/00 ,  C12N9/90 ,  C12N9/86 ,  C07K1/22 ,  C12N9/10

    CPC分类号： C12N15/67 ,  C07K1/22 ,  C12N9/1022 ,  C12N9/86 ,  C12N9/90 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12Y202/01007 ,  C12Y305/02006 ,  C12Y503/03002

    摘要： Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.

    摘要翻译： 提供了一种斜面标签，其能够解决由于在大肠杆菌中表达重组蛋白时外源基因中的密码子与宿主之间的相容性差而导致的翻译率的不稳定性。 与用于解决稀有密码子问题的常规密码子优化或密码子去最佳化方法不同，本发明通过仅使斜坡标签与靶基因融合或独立表达而增加靶蛋白的表达效率，而不改变原始密码子序列 ，从而允许tRNA被重复使用。 因此，与人工合成DNA序列的密码子优化方法相比，本发明提供了增加重组蛋白表达的新方法，其能够降低成本和时间。 因此，预期本发明的方法能够用于生产高附加值药物或工业酶。

    Inhibition of metallo-β-lactamase by RNA
    10.
    发明授权
    Inhibition of metallo-β-lactamase by RNA 有权
    通过RNA抑制金属和β-内酰胺酶

    公开(公告)号：US07989613B2

    公开(公告)日：2011-08-02

    申请号：US11663713

    申请日：2005-10-19

    申请人： Robert W. Shaw ,  Kyu Mee Kim

    发明人： Robert W. Shaw ,  Kyu Mee Kim

    IPC分类号： C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/115 ,  C12Y305/02006

    摘要： Compositions and methods for identifying polyribonucleotides that binds with high affinity to a metallo-β-lactamase. The polyribonucleotides inhibit the activity of the metallo-β-lactamase.

    摘要翻译： 用于鉴定以高亲和力结合金属 - β-内酰胺酶的多核苷酸的组合物和方法。 多核糖核苷酸抑制金属β-内酰胺酶的活性。