摘要:
This invention relates to, in part, compositions of beta-lactamases and methods of using these enzymes in, for example, gastrointestinal tract (GI tract) disorders such as C. difficile infection (CDI).
摘要:
This disclosure relates to the display of proteins and peptides on cellular or non-biotic surfaces in the form of multivalent filamentous polymers. In particular, the disclosure provides for tools and methods for the secretion and functional display of chimeric polypeptides on the surface of cells, in particular, bacterial cells, as well as on foreign substrates, both biological and synthetic. Further envisaged are biotechnological applications using the same.
摘要:
This invention relates to, in part, compositions of beta-lactamases and methods of using these enzymes in, for example, gastrointestinal tract (GI tract) disorders such as C. difficile infection (CDI).
摘要:
The present invention relates to pharmaceuticals and modified beta-lactamases. Specifically, the invention relates to novel recombinant beta-lactamases and pharmaceutical compositions comprising the beta-lactamases.Also, the present invention relates to methods for modifying a beta-lactamase, producing the beta-lactamase and treating or preventing beta-lactam antibiotic induced adverse effects. Furthermore, the present invention relates to the beta-lactamase for use as a medicament and to the use of the beta-lactamase in the manufacture of a medicament for treating or preventing beta-lactam antibiotics induced adverse effects.Still further, the invention relates to a polynucleotide and a host cell comprising the polynucleotide.
摘要:
The disclosure relates to charge-engineered antibodies and penetration-enhanced targeted proteins and their uses for therapeutic treatment or therapeutics delivery.
摘要:
Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in E. coli. Unlike the conventional codon optimization or codon deoptimization method for solving the problem of rare codons, the present invention increases an expression efficiency of a target protein by merely having the ramp tag be fused with a target gene or independently expressed, without changing the original codon sequence, thereby allowing tRNA to be reused. Thus, the present invention provides a novel method for increasing recombinant protein expression which is capable of reducing costs and time in comparison to the codon optimization method that artificially synthesizes DNA sequences. Therefore, it is expected that the method of the present invention will be able to be used in production of high value-added pharmaceuticals or industrial enzymes.
摘要:
Compositions and methods for identifying polyribonucleotides that binds with high affinity to a metallo-β-lactamase. The polyribonucleotides inhibit the activity of the metallo-β-lactamase.
摘要:
Isolated polypeptides are disclosed selected from the group consisting of: (a) polypeptides comprising an amino acid sequence which has at least 90% identity with a sequence of a mature polypeptide comprised in the group of SEQ ID NO: 26 to SEQ ID NO:50; (b) polypeptides which are encoded a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of (i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO: 1 to SEQ ID NO: 25 encoding a mature polypeptide. (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of regions of SEQ ID NO: 1 to SEQ ID NO: 25 encoding a mature polypeptide wherein the polypeptides have a function of the corresponding mature polypeptides comprised in SEQ ID NO:26 to SEQ ID NO:50
摘要翻译:公开了分离的多肽,其选自:(a)包含与SEQ ID NO:26至SEQ ID NO:50组中包含的成熟多肽的序列具有至少90%同一性的氨基酸序列的多肽 ; (b)编码在高严格条件下与选自以下的多核苷酸探针杂交的核苷酸序列的多肽:(i)与选自SEQ ID NO:1至SEQ ID NO:1至SEQ ID NO:1的区域的核苷酸序列的互补链 编号NO:25编码成熟多肽。 (ii)选自编码成熟多肽的SEQ ID NO:1至SEQ ID NO:25的区域的核苷酸序列中包含的cDNA序列的互补链,其中所述多肽具有包含在其中的相应成熟多肽的功能 SEQ ID NO:26至SEQ ID NO:50
摘要:
The present invention related to a method for crystallizing a CMY-10 being a β-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10. With utilization of three-dimensional structure of CMY-10 protein provided by the present invention, it is possible to develop novel antibiotics or inhibitors that can prevent an emergence of resistance bacteria appeared by plasmidic class C β-lactamases having extended-substrate specificity.
摘要:
Methods of and compositions for killing or inhibiting the growth of a bacteria are disclosed. The methods comprise the use of peptide nucleic acids that are targeted to mRNA and/or rRNA. In certain embodiments, methods include the use of one or more separate antibiotics.