公开(公告)号：US5462863A

公开(公告)日：1995-10-31

申请号：US235029

申请日：1994-04-28

Applicant: Jih-Han Hsieh ,  Shu-Ching Shih ,  Wei-Kuang Chi ,  Yi-Ding Chu ,  Ae-Ning Lin

Inventor： Jih-Han Hsieh ,  Shu-Ching Shih ,  Wei-Kuang Chi ,  Yi-Ding Chu ,  Ae-Ning Lin

IPC: C07K14/02 ,  C07K14/085 ,  C07K1/30 ,  C07K1/36 ,  C12N15/51

CPC classification number: C07K14/005 ,  C12N2730/10122 ,  Y10S530/806

Abstract: A method for recovering hepatitis B surface antigen protein from transformed yeast cells including the steps of (i) obtaining an aqueous homogenate of the yeast cells; (ii) enriching the hepatitis B surface antigen protein in the homogenate with a protein-aggregating reagent to form a precipitate which contains hepatitis B surface antigen protein; (iii) dissolving the precipitate in a buffer to form a suspension; and (iv) post-homogenizing the suspension to obtain a 10% to 50% increase in yield of the hepatitis B surface antigen protein as calculated based on a yield achieved without performing the post-homogenizing step.

Abstract translation: 一种从转化的酵母细胞中回收乙型肝炎表面抗原蛋白的方法，包括以下步骤：（i）获得酵母细胞的含水匀浆液; （ii）用蛋白质聚集试剂富集匀浆中的乙型肝炎表面抗原蛋白，形成含有乙型肝炎表面抗原蛋白质的沉淀物; （iii）将沉淀物溶解在缓冲液中以形成悬浮液; 和（iv）将悬浮液均质化以获得基于在不进行后均质化步骤的情况下获得的产量计算的乙型肝炎表面抗原蛋白的产量增加10％至5​​0％。

公开(公告)号：US20190313819A1

公开(公告)日：2019-10-17

申请号：US16149266

申请日：2018-10-02

Applicant: SHU-CHING SHIH ,  CHING-YU Yi

Inventor： Yu-Nien Li

IPC: A47G21/18

Abstract: A paper drinking straw is provided with an elongated member of paper having two short sides and two long sides wherein one long side includes a first joining strip and the other long side includes a second joining strip; a piercing segment at a position of the first joining strip superimposed on the second joining strip; and a cutting segment at one short side and including a first cutting edge connected to one long side, a second cutting edge connected to the first cutting edge and inward inclined relative to the first cutting edge, a third cutting edge connected to the second cutting edge and outward inclined relative to the first cutting edge, and a fourth cutting edge interconnected to the third cutting edge and the other long side. The first joining strip is superimposed on the second joining strip and secured thereto to form the paper drinking straw.

公开(公告)号：US20070275373A1

公开(公告)日：2007-11-29

申请号：US10512253

申请日：2003-04-25

Applicant: Lois Smith ,  Shu-Ching Shih Jaminet

Inventor： Lois Smith ,  Shu-Ching Shih Jaminet

IPC: C12Q1/68 ,  C12N15/63

CPC classification number: C12Q1/6851 ,  C12Q2600/158 ,  C12Q2561/101 ,  C12Q2545/113

Abstract: The present invention is directed to methods of determining a copy number of nucleic acids in a biological specimen. More specifically, the invention provides a method for determining an absolute copy number of a transcript or a plurality of transcripts of interest in a biological specimen the size of which is determined using an external control. The invention further provides a method for diagnosis and prognosis of diseases associated with changes in a transcript copy number by measuring an absolute copy number of the transcript The invention also provides a kit for measuring absolute copy number of a transcript or a plurality or transcripts. The invention further provides a method of creating a quantitative “finger-print” of a disease-related gene expression pattern for screening to identify susceptibility to a disease, and disease diagnostic and prognostic purposes.

Abstract translation: 本发明涉及确定生物样本中核酸拷贝数的方法。 更具体地，本发明提供了一种用于确定生物样本中的转录物或多个感兴趣的转录物的绝对拷贝数的方法，其大小使用外部对照确定。 本发明还提供了通过测量转录本的绝对拷贝数来确定与转录本拷贝数变化相关的疾病的诊断和预后的方法。本发明还提供了用于测定誊本或多个或抄本的绝对拷贝数的试剂盒。 本发明还提供了一种创建疾病相关基因表达模式的定量“指纹”的方法，用于筛选以鉴定对疾病的易感性，以及疾病诊断和预后目的。