利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.012 秒)

    Isolation of Hepatitis B surface antigen from transformed yeast cells
    1.
    发明授权
    Isolation of Hepatitis B surface antigen from transformed yeast cells 失效
    从转化的酵母细胞中分离乙型肝炎表面抗原

    公开(公告)号：US5462863A

    公开(公告)日：1995-10-31

    申请号：US235029

    申请日：1994-04-28

    申请人： Jih-Han Hsieh ,  Shu-Ching Shih ,  Wei-Kuang Chi ,  Yi-Ding Chu ,  Ae-Ning Lin

    发明人： Jih-Han Hsieh ,  Shu-Ching Shih ,  Wei-Kuang Chi ,  Yi-Ding Chu ,  Ae-Ning Lin

    IPC分类号： C07K14/02 ,  C07K14/085 ,  C07K1/30 ,  C07K1/36 ,  C12N15/51

    CPC分类号： C07K14/005 ,  C12N2730/10122 ,  Y10S530/806

    摘要： A method for recovering hepatitis B surface antigen protein from transformed yeast cells including the steps of (i) obtaining an aqueous homogenate of the yeast cells; (ii) enriching the hepatitis B surface antigen protein in the homogenate with a protein-aggregating reagent to form a precipitate which contains hepatitis B surface antigen protein; (iii) dissolving the precipitate in a buffer to form a suspension; and (iv) post-homogenizing the suspension to obtain a 10% to 50% increase in yield of the hepatitis B surface antigen protein as calculated based on a yield achieved without performing the post-homogenizing step.

    摘要翻译： 一种从转化的酵母细胞中回收乙型肝炎表面抗原蛋白的方法，包括以下步骤：（i）获得酵母细胞的含水匀浆液; （ii）用蛋白质聚集试剂富集匀浆中的乙型肝炎表面抗原蛋白，形成含有乙型肝炎表面抗原蛋白质的沉淀物; （iii）将沉淀物溶解在缓冲液中以形成悬浮液; 和（iv）将悬浮液均质化以获得基于在不进行后均质化步骤的情况下获得的产量计算的乙型肝炎表面抗原蛋白的产量增加10％至5​​0％。

    Continuous fermentation process and bioconversion-product recovery
    3.
    发明授权
    Continuous fermentation process and bioconversion-product recovery 失效
    连续发酵过程和生物转化产物回收

    公开(公告)号：US4480034A

    公开(公告)日：1984-10-30

    申请号：US387084

    申请日：1982-06-10

    申请人： Jih-Han Hsieh

    发明人： Jih-Han Hsieh

    IPC分类号： C12P1/00 ,  C12P7/44 ,  C12M1/12 ,  C12M1/36 ,  C12N1/28

    CPC分类号： C12P7/44 ,  C12P1/00 ,  Y10S435/813 ,  Y10S435/877

    摘要： This invention provides a continuous bioconversion process in which a cross-flow membrane filtration zone is employed to recover a whole cell-containing retentate stream and a cell-free bioconversion product-containing permeate stream. The retentate stream is recycled to the fermentation zone. In a specific embodiment, toluene is bio-oxidized to muconic acid with a microorganism such as Pseudomonas putida Biotype A strain ATCC 31,916. The muconic acid is recovered as a precipitate from the cell-free permeate fermentation broth, and the fermentation broth is recycled in the process.

    摘要翻译： 本发明提供一种连续的生物转化方法，其中使用交叉流膜过滤区来回收含有细胞的渗透物流和不含细胞的生物转化产物的渗透物流。 滞留物流被再循环到发酵区。 在一个具体的实施方案中，甲苯与微生物如恶臭假单胞菌生物型A株ATCC 31,916生物氧化成粘康酸。 从无细胞渗透物发酵液中回收粘蛋白作为沉淀物，并在该过程中回收发酵液。

    Continuous fermentation process for aromatic hydrocarbon bioconversion
    4.
    发明授权
    Continuous fermentation process for aromatic hydrocarbon bioconversion 失效
    芳烃生物转化的连续发酵工艺

    公开(公告)号：US4968612A

    公开(公告)日：1990-11-06

    申请号：US635327

    申请日：1984-07-27

    申请人： Jih-Han Hsieh

    发明人： Jih-Han Hsieh

    IPC分类号： C12P7/44

    CPC分类号： C12P7/44 ,  Y10S435/813 ,  Y10S435/875 ,  Y10S435/876 ,  Y10S435/877

    摘要： This invention provides a continuous bioconversion process in which a non-growth toluene substrate is bio-oxidized by a specific microbe mutant strain to accumulated extracellular muconic acid at a bioreactor production rate of at least about 5 grams of muconic acid per liter of fermentation medium per hour.Essential features of the invention process include a continuous feed of whole cell-containing fermentation broth from an auxiliary cell growth and enzyme induction fermentation zone into the main fermentation zone, and a purge stream of whole cell-containing fermentation broth from the main fermentation zone.

    摘要翻译： 本发明提供了一种连续的生物转化方法，其中非生长甲苯底物被特定的微生物突变菌株生物氧化，以生物反应器的生产速率至少约5克粘酸，每升发酵培养基 小时。 本发明方法的基本特征包括从辅助细胞生长和酶诱导发酵区进入主发酵区的全细胞含有发酵液的连续进料，以及来自主发酵区的全细胞含发酵液的清除流。

    Semi-continuous fermentation process for aromatic hydrocarbon bioconversion
    6.
    发明授权
    Semi-continuous fermentation process for aromatic hydrocarbon bioconversion 失效
    用于芳烃生物转化的半连续发酵工艺

    公开(公告)号：US4833078A

    公开(公告)日：1989-05-23

    申请号：US623604

    申请日：1984-06-22

    申请人： Jih-Han Hsieh

    发明人： Jih-Han Hsieh

    IPC分类号： C12P7/44

    CPC分类号： C12P7/44 ,  Y10S435/813 ,  Y10S435/877

    摘要： This invention provides a semi-continuous fermentation process which is operated in a repeated fed-batch mode to maintain cell bioconversion productivity at a high level without product inhibition of enzymatic activity. The process is illustrated by the bioconversion of toluene or catechol via the ortho pathway to muconic acid which accumulates in the fermentation medium in a quantity up to about 50 grams per liter.

    摘要翻译： 本发明提供半连续发酵方法，其以重复补料分批方式操作，以将细胞生物转化生产率维持在高水平，而不抑制酶活性。 该过程通过甲苯或邻苯二酚通过邻位途径生物转化为粘蛋白，其以发酵培养基中积累的量高达约50克/升。

    Process for bioconversion-product recovery
    9.
    发明授权
    Process for bioconversion-product recovery 失效
    生物转化产物回收方法

    公开(公告)号：US4608338A

    公开(公告)日：1986-08-26

    申请号：US387083

    申请日：1982-06-10

    申请人： Jih-Han Hsieh

    发明人： Jih-Han Hsieh

    IPC分类号： C12P1/00 ,  C12P7/40 ,  C12P7/44 ,  C12P7/50 ,  C12P17/04 ,  C12R1/40

    CPC分类号： C12P1/00 ,  C12P7/40 ,  C12P7/44 ,  Y10S435/803 ,  Y10S435/877

    摘要： This invention provides an improved bioconversion system in which a non-growth organic substrate is bio-oxidized to a carboxylic acid product, and the carboxylic acid product is recovered as a precipitate and the resultant fermentation broth is suitable for recycle to the bioreactor. A useful water-insoluble salt is also recovered as a byproduct of the process.

    摘要翻译： 本发明提供了一种改进的生物转化系统，其中非生长有机底物被生物氧化成羧酸产物，羧酸产物作为沉淀物回收，所得发酵液适用于再循环至生物反应器。 还可以回收有用的水不溶性盐作为该方法的副产物。