公开(公告)号：US5462863A

公开(公告)日：1995-10-31

申请号：US235029

申请日：1994-04-28

Applicant: Jih-Han Hsieh ,  Shu-Ching Shih ,  Wei-Kuang Chi ,  Yi-Ding Chu ,  Ae-Ning Lin

Inventor： Jih-Han Hsieh ,  Shu-Ching Shih ,  Wei-Kuang Chi ,  Yi-Ding Chu ,  Ae-Ning Lin

IPC: C07K14/02 ,  C07K14/085 ,  C07K1/30 ,  C07K1/36 ,  C12N15/51

CPC classification number: C07K14/005 ,  C12N2730/10122 ,  Y10S530/806

Abstract: A method for recovering hepatitis B surface antigen protein from transformed yeast cells including the steps of (i) obtaining an aqueous homogenate of the yeast cells; (ii) enriching the hepatitis B surface antigen protein in the homogenate with a protein-aggregating reagent to form a precipitate which contains hepatitis B surface antigen protein; (iii) dissolving the precipitate in a buffer to form a suspension; and (iv) post-homogenizing the suspension to obtain a 10% to 50% increase in yield of the hepatitis B surface antigen protein as calculated based on a yield achieved without performing the post-homogenizing step.

Abstract translation: 一种从转化的酵母细胞中回收乙型肝炎表面抗原蛋白的方法，包括以下步骤：（i）获得酵母细胞的含水匀浆液; （ii）用蛋白质聚集试剂富集匀浆中的乙型肝炎表面抗原蛋白，形成含有乙型肝炎表面抗原蛋白质的沉淀物; （iii）将沉淀物溶解在缓冲液中以形成悬浮液; 和（iv）将悬浮液均质化以获得基于在不进行后均质化步骤的情况下获得的产量计算的乙型肝炎表面抗原蛋白的产量增加10％至5​​0％。