公开(公告)号：US07981646B2

公开(公告)日：2011-07-19

申请号：US10550945

申请日：2004-03-29

申请人： Steve Heald ,  Steve Myers ,  Tim Walford ,  Keith Robbins ,  Colin Hill

发明人： Steve Heald ,  Steve Myers ,  Tim Walford ,  Keith Robbins ,  Colin Hill

IPC分类号： C12P7/04

CPC分类号： C12R1/465 ,  C12P7/24 ,  C12R1/01 ,  Y02P20/544

摘要： Crude solid vanillin-containing material is precipitated from a solution obtained by biotransformation, and purified by a process comprising contacting it with a purification fluid selected from (a) a liquefied gas whose pressure exceeds its critical pressure and whose temperature is below its critical temperature; (b) a supercritical fluid; (c) a gas. The fluid is preferably liquid carbon dioxide. The temperature is maintained below 25°. The product may be further purified by treatment with CO2 in a fluid bed drier. The crude material is preferably one precipitated from a solution resulting from biotransformation of ferulic acid. A new strain of Amycolatopsis capable of generating high concentrations of vanillin with minimal odoriferous by-products (e.g. guaiacol) is also disclosed.

摘要翻译： 从通过生物转化获得的溶液中沉淀含粗固体香草醛的材料，并通过包括使其与选自（a）压力超过其临界压力并且其温度低于其临界温度的液化气体的净化流体接触的方法纯化; （b）超临界流体; （c）气体。 流体优选为液体二氧化碳。 温度保持在25°以下。 可以通过在流化床干燥器中用CO 2处理来进一步纯化产物。 原料优选是从由阿魏酸的生物转化得到的溶液中沉淀出来的。 还公开了能够产生高浓度香草醛的最新气味副产物（例如愈创木酚）的新型菌株。

公开(公告)号：US08563292B2

公开(公告)日：2013-10-22

申请号：US13182515

申请日：2011-07-14

申请人： Steve Heald ,  Steve Myers ,  Tim Walford ,  Keith Robbins ,  Colin Hill

发明人： Steve Heald ,  Steve Myers ,  Tim Walford ,  Keith Robbins ,  Colin Hill

IPC分类号： C12N1/20

CPC分类号： C12R1/465 ,  C12P7/24 ,  C12R1/01 ,  Y02P20/544

摘要： Crude solid vanillin-containing material is precipitated from a solution obtained by biotransformation, and purified by a process comprising contacting it with a purification fluid selected from (a) a liquefied gas whose pressure exceeds its critical pressure and whose temperature is below its critical temperature; (b) a supercritical fluid; (c) a gas. The fluid is preferably liquid carbon dioxide. The temperature is maintained below 25° . The product may be further purified by treatment with CO2 in a fluid bed drier. The crude material is preferably one precipitated from a solution resulting from biotransformation of ferulic acid. A new strain of Amycolatopsis capable of generating high concentrations of vanillin with minimal odoriferous by-products (e.g. guaiacol) is also disclosed.

摘要翻译： 从通过生物转化获得的溶液中沉淀含粗固体香草醛的材料，并通过包括使其与选自（a）压力超过其临界压力并且其温度低于其临界温度的液化气体的净化流体接触的方法纯化; （b）超临界流体; （c）气体。 流体优选为液体二氧化碳。 温度保持在25°以下。 可以通过在流化床干燥器中用CO 2处理来进一步纯化产物。 原料优选是从由阿魏酸的生物转化得到的溶液中沉淀出来的。 还公开了能够产生高浓度香草醛的最新气味副产物（例如愈创木酚）的新型菌株。

公开(公告)号：US20110268858A1

公开(公告)日：2011-11-03

申请号：US13182515

申请日：2011-07-14

申请人： Steve Heald ,  Steve Myers ,  Tim Walford ,  Keith Robbins ,  Colin Hill

发明人： Steve Heald ,  Steve Myers ,  Tim Walford ,  Keith Robbins ,  Colin Hill

IPC分类号： A23L1/221 ,  C12N1/20 ,  C12N1/21

CPC分类号： C12R1/465 ,  C12P7/24 ,  C12R1/01 ,  Y02P20/544

摘要： Crude solid vanillin-containing material is precipitated from a solution obtained by biotransformation, and purified by a process comprising contacting it with a purification fluid selected from (a) a liquefied gas whose pressure exceeds its critical pressure and whose temperature is below its critical temperature; (b) a supercritical fluid; (c) a gas. The fluid is preferably liquid carbon dioxide. The temperature is maintained below 25° . The product may be further purified by treatment with CO2 in a fluid bed drier. The crude material is preferably one precipitated from a solution resulting from biotransformation of ferulic acid. A new strain of Amycolatopsis capable of generating high concentrations of vanillin with minimal odoriferous by-products (e.g. guaiacol) is also disclosed.

摘要翻译： 从通过生物转化获得的溶液中沉淀含粗固体香草醛的材料，并通过包括使其与选自（a）压力超过其临界压力并且其温度低于其临界温度的液化气体的净化流体接触的方法纯化; （b）超临界流体; （c）气体。 流体优选为液体二氧化碳。 温度保持在25°以下。 可以通过在流化床干燥器中用CO 2处理来进一步纯化产物。 原料优选是从由阿魏酸的生物转化得到的溶液中沉淀出来的。 还公开了能够产生高浓度香草醛的最新气味副产物（例如愈创木酚）的新型菌株。

公开(公告)号：US20070298475A1

公开(公告)日：2007-12-27

申请号：US10550945

申请日：2004-03-29

申请人： Steve Heald ,  Myers Steve ,  Tim Walford ,  Keith Robbins ,  Colin Hill

发明人： Steve Heald ,  Myers Steve ,  Tim Walford ,  Keith Robbins ,  Colin Hill

IPC分类号： C12P7/02 ,  C07C47/00 ,  C12N1/20

CPC分类号： C12R1/465 ,  C12P7/24 ,  C12R1/01 ,  Y02P20/544

摘要： Crude solid vanillin-containing material is precipitated from a solution obtained by biotransformation, and purified by a process comprising contacting it with a purification fluid selected from (a) a liquefied gas whose pressure exceeds its critical pressure and whose temperature is below its critical temperature; (b) a supercritical fluid; (c) a gas. The fluid is preferably liquid carbon dioxide. The temperature is maintained below 25°. The product may be further purified by treatment with CO2 in a fluid bed drier. The crude material is preferably one precipitated from a solution resulting from biotransformation of ferulic acid. A new strain of Amycolatopsis capable of generating high concentrations of vanillin with minimal odoriferous by-products (e.g. guaiacol) is also disclosed.

摘要翻译： 从通过生物转化获得的溶液中沉淀含粗固体香草醛的材料，并通过包括使其与选自（a）压力超过其临界压力并且其温度低于其临界温度的液化气体的净化流体接触的方法纯化; （b）超临界流体; （c）气体。 流体优选为液体二氧化碳。 温度保持在25°以下。 可以通过在流化床干燥器中用CO 2 2处理进一步纯化产物。 原料优选是从由阿魏酸的生物转化得到的溶液中沉淀出来的。 还公开了能够产生高浓度香草醛的最新气味副产物（例如愈创木酚）的新型菌株。