Preservation of eukaryotic cells
    1.
    发明申请
    Preservation of eukaryotic cells 有权
    保存真核细胞

    公开(公告)号:US20050084481A1

    公开(公告)日:2005-04-21

    申请号:US10965039

    申请日:2004-10-14

    IPC分类号: A01N1/02 A61K45/00

    CPC分类号: A01N1/0221 A01N1/02

    摘要: A method is disclosed for reliably stabilizing eukaryotic cells that express the P2X7 receptor channel, particularly mammalian and other vertebrate cells, including human cells, for example mammalian macrophages, or hematopoietic stem cells, in order to introduce otherwise membrane-impermeable compounds that are helpful for stabilizing the cells during drying, chilling, freezing, freeze-drying, or cryopreservation. The cells are exposed to extracellular ATP in concentration sufficient to open pores in the plasma membrane. One or more otherwise membrane-impermeable compounds that aid the survivorship of cells are then introduced, for example, trehalose, and after a brief time the pores are closed—for example, by adding divalent cations, or by diluting the extracellular solution. Once the trehalose or other stabilizing compound has been introduced, the cells may be stably preserved. By taking advantage of an endogenous mammalian receptor and ATP, no antigenic compounds need be introduced.

    摘要翻译: 公开了一种用于可靠地稳定表达P2X 7受体通道的真核细胞的方法,特别是哺乳动物和其他脊椎动物细胞,包括人类细胞,例如哺乳动物巨噬细胞或造血干细胞,以便引入其他 有助于在干燥,冷冻,冷冻,冷冻干燥或冷冻保存期间稳定细胞的不透膜化合物。 将细胞以足以打开质膜中的孔的浓度暴露于细胞外ATP。 然后引入一种或多种辅助细胞存活的其它膜不可渗透化合物,例如海藻糖,并且在短暂的时间之后,关闭孔,例如通过加入二价阳离子,或通过稀释细胞外溶液。 一旦引入了海藻糖或其他稳定化合物,就可以稳定地保留细胞。 通过利用内源性哺乳动物受体和ATP,不需要引入抗原性化合物。

    Efficient Synchronization of Element Management Systems to Network Element Attributes
    3.
    发明申请
    Efficient Synchronization of Element Management Systems to Network Element Attributes 有权
    元素管理系统与网络元素属性的高效同步

    公开(公告)号:US20070150620A1

    公开(公告)日:2007-06-28

    申请号:US11426570

    申请日:2006-06-26

    IPC分类号: G06F15/16

    CPC分类号: H04L41/0213 H04L41/00

    摘要: A system, apparatus and a method are described that synchronizes multiple element management systems with a network element. The synchronization between the management entities within the element is maintained by employing special attributes, such as sentry identification for managed objects and guard attributes for managed object tables. Using these attributes, resynchronization between the management systems is improved by reducing the amount of data retrieved by the systems and reducing the processing load caused by the resynchronization process.

    摘要翻译: 描述了将多个元件管理系统与网络元件同步的系统,装置和方法。 元素内的管理实体之间的同步通过采用特殊属性来维护,例如被管理对象的哨兵标识和被管理对象表的保护属性。 使用这些属性,通过减少系统检索的数据量并减少由重新同步过程引起的处理负载,可以改善管理系统之间的重新同步。

    OPTICAL AUTODISCOVERY FOR AUTOMATED LOGICAL AND PHYSICAL CONNECTIVITY CHECK BETWEEN OPTICAL MODULES
    4.
    发明申请
    OPTICAL AUTODISCOVERY FOR AUTOMATED LOGICAL AND PHYSICAL CONNECTIVITY CHECK BETWEEN OPTICAL MODULES 有权
    光学自动化自动化逻辑和物理连接检查光模块之间

    公开(公告)号:US20070092197A1

    公开(公告)日:2007-04-26

    申请号:US11427319

    申请日:2006-06-28

    IPC分类号: G02B6/00

    摘要: Optical autodiscovery is provide between two optical modules to insure that when an optical signal is coupled between the two optical module, the optical signal from a first module does not interfere with operation of a second module. The autodiscovery is implemented by sending an optical identification signal from the first optical module via the coupling to the second optical module from which signal, the second optical module can verify and determined acceptance of the coupled first optical module. During this autodiscovery process, the optical identification signal from the first optical module may be attenuated or shifted in optical spectrum so as not to interfere with the operation of the second optical module. Autodiscovery may also be employed in cases where a first optical module is to receive an optical signal from a second module.

    摘要翻译: 在两个光学模块之间提供光学自动发现,以确保当光信号耦合在两个光学模块之间时,来自第一模块的光信号不会干扰第二模块的操作。 通过从第一光学模块经由耦合向第二光学模块发送光学识别信号来实现自动发现,第二光学模块可从该第二光学模块验证和确定耦合的第一光学模块的接受。 在该自动发现处理中,来自第一光模块的光学识别信号可以在光谱中被衰减或移位,从而不会干扰第二光模块的操作。 在第一光模块将从第二模块接收光信号的情况下也可以采用自动发现。