公开(公告)号：US08518700B2

公开(公告)日：2013-08-27

申请号：US13103611

申请日：2011-05-09

申请人： Seungkwon You ,  Jai-Hee Moon ,  Jun Sung Kim ,  Byung Sun Yoon ,  Jung Han Lee ,  Eun Kyoung Jun ,  June Seok Heo ,  Ji Hyun Kim ,  Ji Hye Hwang ,  Su Hyun Gwon

发明人： Seungkwon You ,  Jai-Hee Moon ,  Jun Sung Kim ,  Byung Sun Yoon ,  Jung Han Lee ,  Eun Kyoung Jun ,  June Seok Heo ,  Ji Hyun Kim ,  Ji Hye Hwang ,  Su Hyun Gwon

IPC分类号： C12N15/85 ,  C12N5/0735

CPC分类号： C12N15/873 ,  C12N5/0696 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/70 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2799/027

摘要： Provided is a composition for reprogramming somatic cells to generate embryonic stem cell-like cells, comprising: a) a Bmi1 (B cell-specific Moloney murine leukemia virus integration site 1) protein or a nucleic acid molecule encoding the Bmi1 protein; and b) at least one low molecular weight substance selected from the group consisting of a set of a MEK/ERK (mitogen-activated protein kinase/extracellular regulated kinase) inhibitor and a GSK (glycogen synthase kinase) inhibitor, a set of a G9a HMTase (G9a histone methyltransferase) inhibitor and a DMNT (DNA methyltransferase) inhibitor, and a histone deacetylase inhibitor. Also, a method is provided for reprogramming somatic cells to generate embryonic stem cell-like cells using the composition. In addition to reducing the number of the reprogramming factors conventionally needed, the composition and method allow the generation of pluripotent embryonic stem cell-like cells which have high potential in the cell therapy of various diseases.

摘要翻译： 提供了一种用于重编程体细胞以产生胚胎干细胞样细胞的组合物，其包含：a）Bmi1（B细胞特异性莫洛尼鼠白血病病毒整合位点1）蛋白或编码Bmi1蛋白的核酸分子; 和b）至少一种选自MEK / ERK（丝裂原活化蛋白激酶/细胞外调节激酶）抑制剂和GSK（糖原合成酶激酶）抑制剂）的一组低分子量物质，一组G9a HMTase（G9a组蛋白甲基转移酶）抑制剂和DMNT（DNA甲基转移酶）抑制剂和组蛋白脱乙酰酶抑制剂。 此外，提供了一种使用该组合物重新编程体细胞以产生胚胎干细胞样细胞的方法。 除了减少常规需要的重新编程因子的数量之外，组合物和方法允许产生在各种疾病的细胞治疗中具有高潜力的多能胚胎干细胞样细胞。

公开(公告)号：US20110275157A1

公开(公告)日：2011-11-10

申请号：US13103611

申请日：2011-05-09

申请人： Seungkwon YOU ,  Jai-Hee MOON ,  Jun Sung KIM ,  Byung Sun YOON ,  Jung Han LEE ,  Eun Kyoung JUN ,  June Seok HEO ,  Ji Hyun KIM ,  Ji Hye HWANG ,  Su Hyun GWON

发明人： Seungkwon YOU ,  Jai-Hee MOON ,  Jun Sung KIM ,  Byung Sun YOON ,  Jung Han LEE ,  Eun Kyoung JUN ,  June Seok HEO ,  Ji Hyun KIM ,  Ji Hye HWANG ,  Su Hyun GWON

IPC分类号： C12N15/85 ,  C12N5/0735

CPC分类号： C12N15/873 ,  C12N5/0696 ,  C12N2501/065 ,  C12N2501/115 ,  C12N2501/70 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2799/027

摘要： Provided is a composition for reprogramming somatic cells to generate embryonic stem cell-like cells, comprising: a) a Bmi1 (B cell-specific Moloney murine leukemia virus integration site 1) protein or a nucleic acid molecule encoding the Bmi1 protein; and b) at least one low molecular weight substance selected from the group consisting of a set of a MEK/ERK (mitogen-activated protein kinase/extracellular regulated kinase) inhibitor and a GSK (glycogen synthase kinase) inhibitor, a set of a G9a HMTase (G9a histone methyltransferase) inhibitor and a DMNT (DNA methyltransferase) inhibitor, and a histone deacetylase inhibitor. Also, a method is provided for reprogramming somatic cells to generate embryonic stem cell-like cells using the composition. In addition to reducing the number of the reprogramming factors conventionally needed, the composition and method allow the generation of pluripotent embryonic stem cell-like cells which have high potential in the cell therapy of various diseases.

摘要翻译： 提供了一种用于重编程体细胞以产生胚胎干细胞样细胞的组合物，其包含：a）Bmi1（B细胞特异性莫洛尼鼠白血病病毒整合位点1）蛋白或编码Bmi1蛋白的核酸分子; 和b）至少一种选自MEK / ERK（丝裂原活化蛋白激酶/细胞外调节激酶）抑制剂和GSK（糖原合成酶激酶）抑制剂）的一组低分子量物质，一组G9a HMTase（G9a组蛋白甲基转移酶）抑制剂和DMNT（DNA甲基转移酶）抑制剂和组蛋白脱乙酰酶抑制剂。 此外，提供了一种使用该组合物重新编程体细胞以产生胚胎干细胞样细胞的方法。 除了减少常规需要的重新编程因子的数量之外，组合物和方法允许产生在各种疾病的细胞治疗中具有高潜力的多能胚胎干细胞样细胞。