公开(公告)号：US20240327796A1

公开(公告)日：2024-10-03

申请号：US18606805

申请日：2024-03-15

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Lorenz STUDER ,  Bastian ZIMMER ,  Jason TCHIEU

IPC分类号： C12N5/0775 ,  A61K35/12 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/079 ,  C12N5/0793

CPC分类号： C12N5/0667 ,  C12N5/0018 ,  C12N5/0606 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0696 ,  A61K35/12 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/04 ,  C12N2510/00 ,  C12N2533/52

摘要： The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into neural crest, cranial placode or non-neuro ectoderm precursors, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating neurodegenerative and pituitary disorders.

公开(公告)号：US12097218B2

公开(公告)日：2024-09-24

申请号：US15989730

申请日：2018-05-25

申请人： Kite Pharma, Inc.

发明人： Eric Gschweng ,  Ruben Rodriguez ,  Yong Ouyang

IPC分类号： A61K35/17 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/0775 ,  C12N5/0783 ,  C12N5/074

CPC分类号： A61K35/17 ,  C12N5/0062 ,  C12N5/0606 ,  C12N5/0636 ,  C12N5/0662 ,  C12N5/0696 ,  C12N2501/125 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/13 ,  C12N2533/52 ,  C12N2533/90

摘要： The disclosure provides a method of generating non-clustered stem cells. Cluster disruption prior to mesoderm differentiation increases yield and efficiency in hEMP and T cell differentiation. Thus, this method allows the development of improved methods of hEMP and T cell differentiation.

公开(公告)号：US20240312569A1

公开(公告)日：2024-09-19

申请号：US18605589

申请日：2024-03-14

申请人： CANON KABUSHIKI KAISHA ,  CANON MEDICAL SYSTEMS CORPORATION

发明人： MIE OKANO ,  ICHIRO HARADA ,  YOJI YAMAMOTO ,  YASUO SAKURAI

IPC分类号： G16B40/10 ,  C12N5/074 ,  G01N21/64

CPC分类号： G16B40/10 ,  C12N5/0696 ,  G01N21/6486

摘要： Provided is a method of stably reprogramming a cell. Provided is an information processing method for reprogramming of a blood cell, the information processing method including an autofluorescence information acquisition step of acquiring autofluorescence information on a subject blood cell and an analysis step of performing analysis based on the autofluorescence information and cell information on the subject blood cell, in which the cell information includes identification information on the blood cell.

公开(公告)号：US20240309334A1

公开(公告)日：2024-09-19

申请号：US18121340

申请日：2023-03-14

申请人： ReGen Theranostics, Inc.

发明人： Timothy Nelson

IPC分类号： C12N5/074 ,  A01N1/02 ,  C12N15/86

CPC分类号： C12N5/0696 ,  A01N1/0284 ,  C12N15/86 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/60 ,  C12N2506/1307 ,  C12N2740/15043

摘要： In some embodiments, the present disclosure is directed to methods of reprogramming fibroblasts into induced pluripotent stem cells. In some embodiments, a method comprises obtaining fibroblasts, such as from a skin biopsy. In some embodiments, a method comprises reprogramming fibroblasts using a viral vector encoding reprogramming factors. In some embodiments, a method comprises expanding iPSC and identifying confluent iPSC.

公开(公告)号：US20240301340A1

公开(公告)日：2024-09-12

申请号：US18667505

申请日：2024-05-17

申请人： SEKISUI CHEMICAL CO., LTD.

发明人： Satoshi HANEDA ,  Yuriko Manabe ,  Ryoma Ishii ,  Hiroki Iguchi ,  Hiroshi Yamauchi ,  Takahiro Omura

IPC分类号： C12M1/12 ,  C08F124/00 ,  C08L79/02 ,  C12M3/00 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12M25/14 ,  C08F124/00 ,  C08L79/02 ,  C12M21/06 ,  C12N5/0068 ,  C12N5/0606 ,  C12N5/0607 ,  C12N5/0696 ,  C08L2666/36 ,  C12N2533/30 ,  C12N2533/70

摘要： A scaffolding material for culturing a stem cell, which contains a synthetic resin, and has a nitrogen content of the synthetic resin of 0.1% by mass or more and 10% by mass or less. According to the scaffolding material for stem cell culture, the scaffolding material can have suitable hydrophilicity and strength, high fixation of stem cells after seeding, and highly efficient cell proliferation.

公开(公告)号：US20240279618A1

公开(公告)日：2024-08-22

申请号：US18571562

申请日：2022-06-21

申请人： SEKISUI CHEMICAL CO., LTD. ,  Epsilon Molecular Engineering, Inc.

发明人： Kenji KASHIWAGI ,  Teruhiko YANAGISAWA ,  Ryo YONEHARA ,  Shigefumi KUMACHI ,  Kanako NAKAO ,  Masayuki TSUCHIYA

IPC分类号： C12N5/074 ,  C07K16/28 ,  C12N5/077 ,  C12N5/0775

CPC分类号： C12N5/0696 ,  C07K16/2863 ,  C12N5/0656 ,  C12N5/0662 ,  C07K2317/569 ,  C07K2317/75 ,  C12N2501/727

摘要： A receptor tyrosine kinase agonist which is a single-domain antibody having a cell growth activity, and is at least one receptor tyrosine kinase agonist selected from the group consisting of a human fibroblast growth factor receptor 1 (FGFR1), a human fibroblast growth factor receptor 2 (FGFR2), a human fibroblast growth factor receptor 3 (FGFR3), and a human fibroblast growth factor receptor 4 (FGFR4).

公开(公告)号：US12054748B2

公开(公告)日：2024-08-06

申请号：US17513253

申请日：2021-10-28

申请人： Children's Medical Center Corporation

发明人： Derrick James Rossi ,  Luigi Warren

IPC分类号： C12N5/00 ,  C07K14/435 ,  C12N5/074 ,  C12N15/11

CPC分类号： C12N5/0696 ,  C07K14/435 ,  C12N15/11 ,  C12N15/111 ,  C12N2310/14 ,  C12N2320/30

摘要： Described herein are synthetic, modified RNAs for changing the phenotype of a cell, such as expressing a polypeptide or altering the developmental potential. Accordingly, provided herein are compositions, methods, and kits comprising synthetic, modified RNAs for changing the phenotype of a cell or cells. These methods, compositions, and kits comprising synthetic, modified RNAs can be used either to express a desired protein in a cell or tissue, or to change the differentiated phenotype of a cell to that of another, desired cell type.

公开(公告)号：US12054745B2

公开(公告)日：2024-08-06

申请号：US17670124

申请日：2022-02-11

申请人： President and Fellows of Harvard College

发明人： Quinn P. Peterson ,  Felicia J. Pagliuca ,  Douglas A. Melton ,  Jeffrey Robert Millman ,  Michael Saris Segel ,  Mads Gurtler

IPC分类号： C12N5/071 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0678 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/22 ,  C12N2506/45

摘要： Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

公开(公告)号：US20240254449A1

公开(公告)日：2024-08-01

申请号：US18626855

申请日：2024-04-04

申请人： EMULATE, INC. ,  CEDARS-SINAI MEDICAL CENTER

发明人： S. Jordan Kerns ,  Norman Wen ,  Carol Lucchesi ,  Christopher David Hinojosa ,  Jacob Fraser ,  Jefferson Puerta ,  Geraldine Hamilton ,  Robert Barrett ,  Clive Svendsen ,  Daniel Levner ,  Stephen R. Targan ,  Michael Workman ,  Dhruv Sareen ,  Uthra Rajamani ,  Magdalena Kasendra

IPC分类号： C12N5/071 ,  C12M3/00 ,  C12M3/06 ,  C12N5/074 ,  C12N5/079 ,  G01N33/50

CPC分类号： C12N5/0679 ,  C12M21/08 ,  C12M23/16 ,  C12N5/0618 ,  C12N5/0696 ,  G01N33/5005 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/24 ,  C12N2501/25 ,  C12N2501/415 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2535/00

摘要： Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

公开(公告)号：US12049641B2

公开(公告)日：2024-07-30

申请号：US16328584

申请日：2017-12-25

申请人： FULLSTEM CO., LTD.

发明人： Shunmei Chiba

IPC分类号： C12N5/0735 ,  C12N5/074 ,  C12N5/0789

CPC分类号： C12N5/0606 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2509/10 ,  C12N2527/00

摘要： Provided is a method for efficiently harvesting cells cultured at a large scale from a three-dimensional porous scaffold. This method includes: a culturing step in which cells are cultured on a three-dimensional porous scaffold sealed in a culture vessel; an enzymatic treatment step in which an enzyme solution containing trypsin-EDTA, caseinase, and/or collagenase is added to the culture vessel to conduct an enzymatic treatment on the cultured cells in the culture vessel; and a vibration treatment step in which a vibration treatment is conducted, i.e., a base that makes a circular motion within a horizontal plane and a bottom surface of the culture vessel are made to come into surface contact with one another so as to impart a vertical-direction vibration to the cultured cells that have undergone the enzymatic treatment.