公开(公告)号：US06555376B2

公开(公告)日：2003-04-29

申请号：US10201247

申请日：2002-07-24

申请人： Amarnath Maitra ,  Subho Mozumdar ,  Susmita Mitra ,  Indrajit Roy

发明人： Amarnath Maitra ,  Subho Mozumdar ,  Susmita Mitra ,  Indrajit Roy

IPC分类号： C12N1588

CPC分类号： B82Y5/00 ,  A61K47/549 ,  A61K47/6923 ,  A61K47/6929 ,  C12N15/88

摘要： The present invention relates to a process of entrapping genetic materials in nanoparticles of inorganic metal salts of size below 100 nm diameter to form non-viral carriers for delivery of genes. The process comprises the steps of dissolving surfactants and a cosurfactant in oil to obtain reverse micelles. An aqueous solution of genetic material is added to the reverse micelles. Thereafter the reverse micelles are divided into two equal parts. To one part, aqueous solution of inorganic metal salts is dissolved to obtain optically clear and transparent reverse micelles. To the second part aqueous solution of precipitating agent is added to obtain optically clear and transparent reverse micelles. The two equally divided parts of reverse micelles are mixed to form inorganic nanopartcles encapsulating added genetic material. Thereafter, the nanoparticles are separated from reverse micelles, the inorganic nanoparticles are dispersed in water and dialyzed to remove free metal salts, surfactant and oil.

摘要翻译： 本发明涉及一种在大于100nm直径的无机金属盐的纳米颗粒中捕获遗传物质以形成用于递送基因的非病毒载体的方法。 该方法包括将表面活性剂和辅助表面活性剂溶解在油中以获得反胶束的步骤。 将遗传物质的水溶液加入到反胶束中。 此后将反胶束分成两等份。 一方面，将无机金属盐的水溶液溶解，得到透明透明的反胶束。 向第二部分添加沉淀剂水溶液以获得光学透明且透明的反胶束。 将两个等分的部分反胶束混合以形成包封添加的遗传物质的无机纳米颗粒。 此后，将纳米颗粒与反胶束分离，将无机纳米颗粒分散在水中并透析以除去游离金属盐，表面活性剂和油。