公开(公告)号：US08383417B2

公开(公告)日：2013-02-26

申请号：US12644284

申请日：2009-12-22

申请人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

发明人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

IPC分类号： G01N24/00 ,  G01N33/00

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。

公开(公告)号：US20120064558A1

公开(公告)日：2012-03-15

申请号：US12881115

申请日：2010-09-13

申请人： Mary Frances LOPEZ ,  Amol PRAKASH ,  David A. SARRACINO ,  Taha REZAI ,  Bryan E. KRASTINS ,  Michael J. ATHANAS

发明人： Mary Frances LOPEZ ,  Amol PRAKASH ,  David A. SARRACINO ,  Taha REZAI ,  Bryan E. KRASTINS ,  Michael J. ATHANAS

IPC分类号： C12Q1/37

CPC分类号： G01N33/92 ,  C07K14/775 ,  G01N33/6896 ,  G01N2333/775 ,  G01N2800/2871

摘要： Methods are disclosed for rapidly and reliably differentiating between ischemic and hemorrhagic stroke events in a human patient. The methods include obtaining a biological sample, such as blood serum, and analyzing the sample or a derivative thereof by mass spectrometry to measure the amount of one or more targeted proteins differentially present and ischemic and hemorrhagic stroke patients. The analysis may include proteolytic digestion of the biological sample and selective reaction monitoring (SRM) tandem mass spectrometry of the digested sample to determine the amounts of surrogate peptides corresponding to the targeted proteins.

摘要翻译： 公开了用于在人类患者中快速和可靠地区分缺血性和出血性中风事件之间的方法。 所述方法包括获得诸如血清的生物样品，并通过质谱分析样品或其衍生物以测量差异存在的一种或多种靶向蛋白质和缺血性和出血性中风患者的量。 分析可以包括生物样品的蛋白水解消化和消化样品的选择性反应监测（SRM）串联质谱法，以确定与靶向蛋白质相对应的替代肽的量。

公开(公告)号：US20110151568A1

公开(公告)日：2011-06-23

申请号：US12644284

申请日：2009-12-22

申请人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

发明人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

IPC分类号： G01N33/00

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。

公开(公告)号：US20130217058A1

公开(公告)日：2013-08-22

申请号：US13776449

申请日：2013-02-25

申请人： Mary Frances LOPEZ ,  David A. SARRACINO ,  Amol PRAKASH ,  Randall W. NELSON ,  Paul ORAN ,  Bryan E. KRASTINS ,  Taha REZAI

发明人： Mary Frances LOPEZ ,  David A. SARRACINO ,  Amol PRAKASH ,  Randall W. NELSON ,  Paul ORAN ,  Bryan E. KRASTINS ,  Taha REZAI

IPC分类号： G01N27/62

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。

公开(公告)号：US20100216250A1

公开(公告)日：2010-08-26

申请号：US12710062

申请日：2010-02-22

申请人： Mary Frances LOPEZ ,  David A. Sarracino ,  Bryan E. Krastins ,  Taha Rezai ,  Amol Prakash ,  Michael J. Athanas

发明人： Mary Frances LOPEZ ,  David A. Sarracino ,  Bryan E. Krastins ,  Taha Rezai ,  Amol Prakash ,  Michael J. Athanas

IPC分类号： G01N33/50

CPC分类号： G01N33/689 ,  G01N33/6848 ,  G01N2333/775 ,  G01N2333/805 ,  G01N2800/387

摘要： The present invention relates to biomarkers in maternal serum samples and methods for predicting whether a fetus has Trisomy 21 during the first trimester of pregnancy based on the differential expression of a combination, or subcombination, of the same.

摘要翻译： 本发明涉及母体血清样品中的生物标志物和基于组合的差异表达或其亚组合来预测胎儿在妊娠前三个月是否具有三体性21的方法。