公开(公告)号：US20100216250A1

公开(公告)日：2010-08-26

申请号：US12710062

申请日：2010-02-22

申请人： Mary Frances LOPEZ ,  David A. Sarracino ,  Bryan E. Krastins ,  Taha Rezai ,  Amol Prakash ,  Michael J. Athanas

发明人： Mary Frances LOPEZ ,  David A. Sarracino ,  Bryan E. Krastins ,  Taha Rezai ,  Amol Prakash ,  Michael J. Athanas

IPC分类号： G01N33/50

CPC分类号： G01N33/689 ,  G01N33/6848 ,  G01N2333/775 ,  G01N2333/805 ,  G01N2800/387

摘要： The present invention relates to biomarkers in maternal serum samples and methods for predicting whether a fetus has Trisomy 21 during the first trimester of pregnancy based on the differential expression of a combination, or subcombination, of the same.

摘要翻译： 本发明涉及母体血清样品中的生物标志物和基于组合的差异表达或其亚组合来预测胎儿在妊娠前三个月是否具有三体性21的方法。

公开(公告)号：US20110151568A1

公开(公告)日：2011-06-23

申请号：US12644284

申请日：2009-12-22

申请人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

发明人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

IPC分类号： G01N33/00

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。

公开(公告)号：US08383417B2

公开(公告)日：2013-02-26

申请号：US12644284

申请日：2009-12-22

申请人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

发明人： Mary Frances Lopez ,  David A. Sarracino ,  Amol Prakash ,  Randall W. Nelson ,  Paul Oran ,  Bryan E. Krastins ,  Taha Rezai

IPC分类号： G01N24/00 ,  G01N33/00

CPC分类号： G01N27/62 ,  G01N33/78 ,  G01N2560/00 ,  Y10T436/24

摘要： Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay.

摘要翻译： 描述了用于通过消化样品以产生对目标PTH变体特异性的替代肽并通过选择性反应监测（SRM）质谱法检测和定量替代肽的方法来监测生物样品中PTH变体的量，使用一组 针对灵敏度和选择性优化的前体 - 产物离子转移。 可以通过免疫亲和捕获或其它合适的技术将PTH变体或其部分浓缩在样品中。 本文描述的质谱法可以在单次测定中同时测量代表多种靶向PTH变体的肽。

公开(公告)号：US09897581B1

公开(公告)日：2018-02-20

申请号：US15498114

申请日：2017-04-26

申请人： Thermo Finnigan LLC ,  Amol Prakash

发明人： Amol Prakash ,  Scott M. Peterman ,  David Sarracino

IPC分类号： H01J49/00 ,  G01N30/72 ,  G01N33/50 ,  H01J49/42

CPC分类号： G01N30/7233 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/425

摘要： A variable data dependent acquisition/dynamic exclusion (vDDA/DE) method selects target m/z range utilizing a MS1 precursor topography map over the most recently acquired MS spectrum to identify the precursor m/z values and MS/MS acquisition parameters to improve the selection of the next data-dependent MS/MS acquisition. The topography used to define the next set of DDA scan events is defined by previous tandem MS scan events defined by precursor quadrupole isolation windows as well as all detected compounds contained within the specific tandem MS events. At least some of the parameters used for MS/MS data acquisition are dynamic so as to exhaustively sample the user specified MS mass range with MS/MS information. These parameters include the quadrupole MS isolation width and symmetry around the targeted m/z value. Using this approach, a greater proportion of the precursor m/z space is effectively and efficiently sampled per chromatographic peak width.

公开(公告)号：US06377805B1

公开(公告)日：2002-04-23

申请号：US09368120

申请日：1999-08-04

申请人： Dinesh Kashinath Anvekar ,  Manpreet Singh Dang ,  Amol Prakash ,  Rajeev Shorey

发明人： Dinesh Kashinath Anvekar ,  Manpreet Singh Dang ,  Amol Prakash ,  Rajeev Shorey

IPC分类号： H04Q720

CPC分类号： H04W36/08 ,  H04W84/18 ,  H04W88/04

摘要： A data communication for a handoff mobile unit is maintained by data forwarding through neighboring mobile units in indoor wireless cellular communication networks operates such that, when the master unit has no available channels, the mobile unit switches over to master status to create an ad hoc cell. The mobile unit then pages neighboring slave mobile units for data forwarding assistance. The mobile unit chooses one or more slave mobile units and establishes wireless links with the chosen slave mobile units. These chosen mobile units then have two masters; one the master unit of the cell and the other the handoff mobile unit which has created the ad hoc cell. A list of the slave mobile units in the ad hoc cell is sent to the network server, and data communication proceeds with data forwarding through the chosen mobile slave units. When a channel becomes available with the master unit for the cell, the handoff mobile unit becomes a slave mobile unit to the master unit and the ad hoc cell is dissolved.

摘要翻译： 通过在室内无线蜂窝通信网络中通过相邻移动单元的数据转发来维护用于切换移动单元的数据通信操作，使得当主单元没有可用信道时，移动单元切换到主状态以创建自组织小区 。 移动单元然后对相邻的从动单元进行页面数据转发协助。 移动单元选择一个或多个从属移动单元并与所选择的从属移动单元建立无线链路。 这些选择的移动单元然后有两个主人; 一个是小区的主单元，另一个是已经创建了自组织小区的切换移动单元。 Ad hoc小区中的从属移动单元的列表被发送到网络服务器，并且数据通信通过所选择的移动从属单元进行数据转发。 当信道对于小区的主单元可用时，切换移动单元变为主单元的从属移动单元，并且ad hoc小区被解散。

公开(公告)号：US08298829B2

公开(公告)日：2012-10-30

申请号：US13013163

申请日：2011-01-25

申请人： Amol Prakash

发明人： Amol Prakash

IPC分类号： B01D59/44 ,  B01D59/00

CPC分类号： G01N30/8693 ,  G01N30/8644 ,  G01N30/8675 ,  G01N33/6803 ,  G01N33/6848

摘要： A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions.

摘要翻译： 用于从液相色谱柱确定感兴趣的肽的时间的方法包括获得感兴趣的肽的多个候选碎片离子中的每一个的色谱数据的步骤。 沿着常见色谱时间的时间是对应于针对多个候选碎片离子中的每一个测量的离子信号的最大重叠而确定的刻度。 最后，确定的时间被指定为目的肽从液相色谱柱洗脱的时间。 特别地，在从含有感兴趣的肽的液相色谱柱的洗脱液的选择性反应监测期间获取色谱数据。 色谱数据包括沿着多个候选碎片离子中的每一个的公共色谱时间标度测量的离子信号。

公开(公告)号：US06594475B1

公开(公告)日：2003-07-15

申请号：US09392426

申请日：1999-09-09

申请人： Dinesh Kashinath Anvekar ,  Manpreet Singh Dang ,  Amol Prakash

发明人： Dinesh Kashinath Anvekar ,  Manpreet Singh Dang ,  Amol Prakash

IPC分类号： H04Q720

CPC分类号： H04W52/24 ,  H04B7/0808 ,  H04W24/00 ,  H04W52/0219 ,  H04W52/0245 ,  H04W84/10 ,  Y02D70/444

摘要： A method and apparatus minimize battery discharge of mobile communication devices in short range wireless radio communication networks by switching receiving antennas to minimize transmitted power for a mobile communication device. Multiple receiving antennas and at least one transmitting antenna are used. The receiving antennas are switched for each mobile communication device to minimize transmitted power required by a mobile communication device. Switching between receiving antennas is accomplished by measuring power of a signal received from a mobile communication device at each receiving antenna, and selecting a receiving antenna based on the measured power. A database of switchings is maintained for the mobile communication devices, and an initial selection of a receiving antenna is based on the last switching or a trend indicated by most recent switchings. Alternatively, the initial switching is to a centrally located receiving antenna.

摘要翻译： 一种方法和装置通过切换接收天线来最小化移动通信设备的发射功率来使短距离无线电通信网络中的移动通信设备的电池放电最小化。 使用多个接收天线和至少一个发射天线。 为每个移动通信设备切换接收天线，以最小化移动通信设备所需的发射功率。 在接收天线之间的切换是通过测量在每个接收天线处从移动通信设备接收的信号的功率，并且基于测量的功率选择接收天线来实现的。 保持移动通信设备的切换数据库，并且接收天线的初始选择是基于最近的切换或由最近切换指示的趋势。 或者，初始切换到位于中心的接收天线。

公开(公告)号：US10082490B1

公开(公告)日：2018-09-25

申请号：US15898901

申请日：2018-02-19

申请人： Thermo Finnigan LLC ,  Amol Prakash

发明人： Amol Prakash ,  Scott M. Peterman ,  David Sarracino

IPC分类号： H01J49/00 ,  G01N30/72 ,  G01N33/50 ,  H01J49/42

CPC分类号： G01N30/7233 ,  G01N33/50 ,  G01N33/6848 ,  G01N2560/00 ,  H01J49/0027 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/425

摘要： A variable data dependent acquisition/dynamic exclusion (vDDA/DE) method selects target m/z range utilizing a MS1 precursor topography map over the most recently acquired MS spectrum to identify the precursor m/z values and MS/MS acquisition parameters to improve the selection of the next data-dependent MS/MS acquisition. The topography used to define the next set of DDA scan events is defined by previous tandem MS scan events defined by precursor quadrupole isolation windows as well as all detected compounds contained within the specific tandem MS events. At least some of the parameters used for MS/MS data acquisition are dynamic so as to exhaustively sample the user specified MS mass range with MS/MS information. These parameters include the quadrupole MS isolation width and symmetry around the targeted m/z value. Using this approach, a greater proportion of the precursor m/z space is effectively and efficiently sampled per chromatographic peak width.

公开(公告)号：US07897405B2

公开(公告)日：2011-03-01

申请号：US12029393

申请日：2008-02-11

申请人： Amol Prakash

发明人： Amol Prakash

IPC分类号： G01N33/00 ,  G06F19/00

CPC分类号： G01N30/8693 ,  G01N30/8644 ,  G01N30/8675 ,  G01N33/6803 ,  G01N33/6848

摘要： A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions.

摘要翻译： 用于从液相色谱柱确定感兴趣的肽的时间的方法包括获得感兴趣的肽的多个候选碎片离子中的每一个的色谱数据的步骤。 沿着常见色谱时间的时间是对应于针对多个候选碎片离子中的每一个测量的离子信号的最大重叠而确定的刻度。 最后，确定的时间被指定为目的肽从液相色谱柱洗脱的时间。 特别地，在从含有感兴趣的肽的液相色谱柱的洗脱液的选择性反应监测期间获取色谱数据。 色谱数据包括沿着多个候选碎片离子中的每一个的公共色谱时间标度测量的离子信号。

公开(公告)号：US20090326828A1

公开(公告)日：2009-12-31

申请号：US12163928

申请日：2008-06-27

申请人： Amol Prakash

发明人： Amol Prakash

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  G01N2500/00 ,  H01J49/00

摘要： Methods for selecting a set of SRM transitions for a peptide of interest include selecting a first transition based on sensitivity criteria and selecting at least a second transition based on selectivity criteria. A determination of the uniqueness of the first transition combined with the at least a second transition is made. When the combination of the first transition and the at least a second transition is determined to be unique to the peptide of interest, a sample containing the peptide of interest is subjected to a SRM workflow by monitoring the first transition and the second transition. Also described is an apparatus for carrying out the methods.

摘要翻译： 用于选择感兴趣的肽的一组SRM转换的方法包括基于灵敏度标准选择第一转换并基于选择性标准选择至少第二转换。 进行与至少第二过渡组合的第一过渡的唯一性的确定。 当确定第一转变和至少第二转化的组合对感兴趣的肽是唯一的时，通过监测第一转化和第二转化，将含有感兴趣肽的样品经受SRM工作流程。 还描述了一种用于执行这些方法的装置。