公开(公告)号：US5378831A

公开(公告)日：1995-01-03

申请号：US941302

申请日：1992-09-04

申请人： Taichi Usui ,  Osamu Uejima ,  Koichi Ogawa

发明人： Taichi Usui ,  Osamu Uejima ,  Koichi Ogawa

IPC分类号： G01N33/573 ,  C07H3/06 ,  C07H15/203 ,  C08B37/00 ,  C12P19/14 ,  C12P19/18 ,  C12Q1/40 ,  C07G17/00 ,  C07G3/00 ,  C07H1/00

CPC分类号： C07H3/06 ,  C12P19/14

摘要： Galactosyl maltooligosaccharide derivatives represented by formula (1): ##STR1## wherein at least one of X.sub.1 and X.sub.2 represents a galactosyl group and the other represents a hydrogen atom, R represents a hydrogen atom or a substituted or unsubstituted phenyl group, and n is an integer of from 2 to 5. The derivatives are prepared by reacting a sugar with a galactosyl group with a maltooligosaccharide represented by formula (2): ##STR2## R represents a hydrogen atom or a substituted or unsubstituted phenyl group, and n is an integer of from 2 to 5; in the presence of .alpha.-galactosidase or .beta.-galactosidase. The derivatives are used for measurement of .alpha.-amylase activity.

摘要翻译： 由式（1）表示的半乳糖基麦芽低聚糖衍生物：其中X1和X2中的至少一个表示半乳糖基，另一个表示氢原子，R表示氢原子或取代或未取代的苯基，n为 通过使糖与半乳糖基与式（2）表示的麦芽低聚糖反应制备衍生物：R表示氢原子或取代或未取代的苯基，n为整数 从2到5; 在α-半乳糖苷酶或β-半乳糖苷酶的存在下。 衍生物用于测定α-淀粉酶活性。

公开(公告)号：US07109014B1

公开(公告)日：2006-09-19

申请号：US09806413

申请日：1999-09-29

申请人： Shigeru Yamamoto ,  Masamichi Okada ,  Taichi Usui ,  Kanzo Sakata ,  Atsuki Toumoto ,  Kazutaka Tsuruhami

发明人： Shigeru Yamamoto ,  Masamichi Okada ,  Taichi Usui ,  Kanzo Sakata ,  Atsuki Toumoto ,  Kazutaka Tsuruhami

IPC分类号： C12N9/26

CPC分类号： C12Y302/01149 ,  C12N9/2402 ,  C12P19/12 ,  C12Y302/01021

摘要： This invention relates to a novel microorganism-derived enzyme having an activity to cut disaccharide glycosides (particularly, β-primeveroside and/or analogous disaccharide glycosides) in disaccharide unit, a method for producing the enzyme, a gene which encodes the enzyme and use of the enzyme. Various components can be formed by the action of this enzyme upon disaccharide glycosides.

摘要翻译： 本发明涉及一种具有二糖单位切割二糖苷（特别是β-主要糖苷和/或类似二糖苷）活性的新型微生物来源的酶，该酶的制备方法，编码该酶的基因及其用途 该酶。 可以通过该酶对二糖苷的作用形成各种成分。

公开(公告)号：US5208151A

公开(公告)日：1993-05-04

申请号：US607612

申请日：1990-10-31

申请人： Taichi Usui ,  Teruo Nakakuki ,  Kazuo Sakai

发明人： Taichi Usui ,  Teruo Nakakuki ,  Kazuo Sakai

IPC分类号： C12P19/14 ,  C12Q1/40

CPC分类号： C12Q1/40 ,  C12P19/14 ,  C12Q2334/00 ,  C12Q2334/10

摘要： Highly purified derivatives of maltooligosaccharides can be produced at a high yield by reacting, in a mixture of a hydrophilic organic solvent and water, a mixture of maltooligosaccharides or a substance capable of being converted into the maltooligosaccharides upon reaction with an amylase, and an o-glucosyl derivative, with the amylase.The resulting derivatives of maltooligosaccharides are useful as a substrate for the determination of .alpha.-amylase activity in a humor, physiologically active substances, natural dieteic sweetenings, coloring agents and the like.

摘要翻译： 通过在亲水性有机溶剂和水的混合物中与麦芽低聚糖或能与淀粉酶反应后能够转化成麦芽低聚糖的物质的混合物反应，可高产率地制备麦芽低聚糖的高度纯化的衍生物， 葡萄糖基衍生物，与淀粉酶。 所得到的麦芽低聚糖衍生物可用作测定幽默，生理活性物质，天然饮食甜味剂，着色剂等中α-淀粉酶活性的底物。