公开(公告)号：US06458593B1

公开(公告)日：2002-10-01

申请号：US09489085

申请日：2000-01-21

申请人： James R. Musick ,  John Charles Gill, IV ,  Bruce P. Burnett ,  Tammy E. Hedlund

发明人： James R. Musick ,  John Charles Gill, IV ,  Bruce P. Burnett ,  Tammy E. Hedlund

IPC分类号： C12N1564

CPC分类号： C12N5/0616 ,  C12N2500/25 ,  C12N2501/11 ,  C12N2501/16 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2509/00 ,  C12N2510/02 ,  C12N2510/04

摘要： The present invention relates to methods of immortalizing various primary cell cultures, including pituitary cells, neurons, beta islet cells, glial cells, corneal epithelial cells and follicular stellate cells. The primary cells are transfected with a vector containing an establishment oncogene, resulting in non-transformed immortalized cells. The primary cells and/or the subsequent immortalized cells are cultured in a defined media containing one or more environmental factor(s) that control the proliferation and/or differentiation of the cells.

摘要翻译： 本发明涉及永生化各种原代细胞培养物，包括垂体细胞，神经元，胰岛细胞，胶质细胞，角膜上皮细胞和滤泡星状细胞的方法。 用含有致癌基因的载体转染原代细胞，导致未转化的永生化细胞。 将原代细胞和/或随后的永生化细胞在含有一种或多种控制细胞增殖和/或分化的环境因子的限定培养基中培养。