公开(公告)号：US20110189670A1

公开(公告)日：2011-08-04

申请号：US13002944

申请日：2009-07-07

申请人： Ruth L Katz ,  Abha Khanna ,  Tanweer M. Zaidi ,  Weigong He ,  Ricardo L. Fernandez ,  Ivan Gorlov

发明人： Ruth L Katz ,  Abha Khanna ,  Tanweer M. Zaidi ,  Weigong He ,  Ricardo L. Fernandez ,  Ivan Gorlov

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q1/6841 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/136 ,  G01N33/57492 ,  G01N2333/70589

摘要： The present invention comprises a method of detecting circular tumor cells and methods of detecting, evaluating, or staging cancer in a patient, as well as a method of monitoring treatment of cancer in a patient using the claimed method. The method comprises contacting a sample with a CD45 binding agent; selecting the cells based on positive or negative CD45 staining; contacting the selected cells with a labeled nucleic acid probe, and detecting hybridized cells by fluorescence in situ hybridization; and analyzing a signal produced by the labels on the hybridized cells to detect the CTCs. In other embodiments, the method provides for directed to a method of determining the level of CTCs in a sample having blood cells from a patient by contacting a sample having blood cells from a patient, wherein the sample has not been pre-sorted into CD45-positive and CD45-negative cells.

摘要翻译： 本发明包括检测圆形肿瘤细胞的方法以及检测，评价或分期患者癌症的方法以及使用所要求保护的方法监测患者癌症的方法。 该方法包括使样品与CD45结合剂接触; 基于阳性或阴性CD45染色选择细胞; 使所选择的细胞与标记的核酸探针接触，并通过荧光原位杂交检测杂交细胞; 并分析由杂交细胞上的标记产生的信号以检测CTC。 在其它实施方案中，该方法提供了一种通过使来自患者具有血细胞的样品接触来确定样品中具有血细胞的样品中CTC水平的方法，其中样品尚未预分选成CD45- 阳性和CD45阴性细胞。

公开(公告)号：US20100317002A1

公开(公告)日：2010-12-16

申请号：US12682594

申请日：2008-10-06

申请人： Michal Daniely ,  Lea Madi ,  Tal Kaplan ,  Boaz Pal ,  Yuval Harari ,  Tanweer M. Zaidi ,  Ricardo L. Fernandez ,  Jinping Zhang ,  Ruth Katz

发明人： Michal Daniely ,  Lea Madi ,  Tal Kaplan ,  Boaz Pal ,  Yuval Harari ,  Tanweer M. Zaidi ,  Ricardo L. Fernandez ,  Jinping Zhang ,  Ruth Katz

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6841 ,  C12Q2565/626 ,  C12Q2563/173

摘要： Provided is a method of identifying a genetically abnormal cell in a sputum sample, the method comprising: (a) staining a sputum sample using a morphological stain so as to identify a lower airway tract cell or lung cell in the sputum sample; and (b) staining the sputum sample using fluorescent in situ hybridization (FISH) so as to identify in the lower airway tract cell or lung cell a genetic abnormality in at least one of human chromosome 3p22.1 and 10q22-23, thereby identifying the genetically abnormal cell in the sputum sample. Also provided are methods and kits of diagnosing lung cancer by detecting a presence of genetically abnormal cells above a predetermined threshold in a sputum sample.

摘要翻译： 提供了一种鉴定痰样品中的遗传异常细胞的方法，所述方法包括：（a）使用形态染色染色痰样品，以鉴定痰样品中的下呼吸道细胞或肺细胞; 和（b）使用荧光原位杂交（FISH）对痰样品进行染色，以在下呼吸道细胞或肺细胞中识别人染色体3p22.1和10q22-23中的至少一个的遗传异常，从而鉴定 遗传异常细胞在痰样品。 还提供了通过在痰样品中检测高于预定阈值的遗传异常细胞的存在来诊断肺癌的方法和试剂盒。