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    DETECTION METHOD AND DETECTION KIT FOR NUCLEIC ACID MOLECULES
    2.
    发明申请
    DETECTION METHOD AND DETECTION KIT FOR NUCLEIC ACID MOLECULES 审中-公开

    公开(公告)号：US20180251821A1

    公开(公告)日：2018-09-06

    申请号：US15660226

    申请日：2017-07-26

    申请人： CHANG GUNG UNIVERSITY

    发明人： Chiuan-Chian CHIOU ,  Tai-Long CHEN ,  Ji-Dung LUO

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Q2537/119 ,  C12Q2563/173

    摘要： A method for detecting a target nucleic acid fragment includes the following steps: preparing a nucleic acid probe and an intercalating dye configured to bind to double-stranded nucleic acids, wherein the nucleic acid probe includes a nucleic acid chain, a fluorescent reporter, and a fluorescent quencher, the fluorescent reporter is conjugated to a first end of the nucleic acid chain, and the fluorescent quencher is conjugated to a second end of the nucleic acid chain opposing to the first end; binding the nucleic acid probe to the target nucleic acid fragment to form a partial double stranded structure; intercalating the intercalating dye in the partial double stranded structure, so that, after the intercalating dye is excited by an irradiation, the fluorescent reporter is excited through the intercalating dye to emit a fluorescence signal; and detecting the target nucleic acid fragment according to the fluorescence signal.

    Colorimetric Detection of Nucleic Acid Amplification
    7.
    发明申请
    Colorimetric Detection of Nucleic Acid Amplification 审中-公开
    核酸扩增的比色检测

    公开(公告)号：US20170044599A1

    公开(公告)日：2017-02-16

    申请号：US15306240

    申请日：2015-04-24

    申请人： DIASSESS INC.

    发明人： Debkishore Mitra ,  Ivan Krastev Dimov ,  John Robert Waldeisen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q1/6846 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2563/173

    摘要： Colorimetry is used to detect amplification reaction products. A sample is contacted with a reaction mix under conditions such that an amplification reaction occurs and produces an amplification reaction product if the sample contains a target nucleic acid template molecule. The reaction mix includes an enzyme for catalyzing the amplification reaction, and at least one halochromic agent. If the target nucleic acid template molecule is present, the amplification reaction changes the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating the presence of the target nucleic acid. If the target nucleic acid template molecule is not present, the amplification reaction does not generate an adequate number of protons to sufficiently change the starting pH of the reaction mix to cause a detectable colorimetric change of the halochromic agent, thereby indicating that the amplification reaction product has not been produced.

    摘要翻译： 比色法用于检测扩增反应产物。 在发生扩增反应的条件下使样品与反应混合物接触，如果样品含有靶核酸模板分子，则产生扩增反应产物。 反应混合物包括用于催化扩增反应的酶和至少一种卤代色素。 如果存在靶核酸模板分子，则扩增反应改变反应混合物的起始pH，以引起卤色变色剂的可检测的比色变化，从而指示靶核酸的存在。 如果目标核酸模板分子不存在，则扩增反应不产生足够数量的质子，以充分改变反应混合物的起始pH，引起卤代色素的可检测的比色变化，从而表明扩增反应产物 尚未制作。

    NUCLEIC ACID DETECTION AND QUANTIFICATION
    8.
    发明申请
    NUCLEIC ACID DETECTION AND QUANTIFICATION 审中-公开
    核酸检测和定量

    公开(公告)号：US20160298182A1

    公开(公告)日：2016-10-13

    申请号：US15102685

    申请日：2014-12-09

    申请人： QIAGEN GMBH

    发明人： Nan Fang ,  Andreas Missel ,  Katja Heitz ,  Holger Wedler

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q2561/113 ,  C12Q2563/107 ,  C12Q2563/173 ,  C12Q2565/1025

    摘要： The present invention relates to methods and uses for the detection or quantification of newly-synthesized double-stranded target nucleic acid molecules in a sample during quantitative real-time polymerase chain reaction (qPCR) amplification. According to the invention, an intercalating dye recognizing double-stranded DNA molecules with higher affinity than single-stranded DNA molecules and a fluorophore-labeled oligonucleotide-probe being sequence specific for a target nucleic acid molecule are simultaneously employed, thus enabling quantification a specific target and total amount of a mixed nucleic acid population, and enabling assessing the cause of suboptimal PCR performance.

    摘要翻译： 本发明涉及用于在定量实时聚合酶链反应（qPCR）扩增过程中检测或定量样品中新合成的双链靶核酸分子的方法和用途。 根据本发明，同时使用识别比单链DNA分子更高亲和力的双链DNA分子的插入染料和靶核酸分子具有序列特异性的荧光团标记的寡核苷酸探针，从而能够定量特异性靶 和混合核酸群体的总量，并且能够评估次优PCR性能的原因。

    QUANTIFICATION OF RNA
    9.
    发明申请
    QUANTIFICATION OF RNA 有权
    RNA的定量

    公开(公告)号：US20160244821A1

    公开(公告)日：2016-08-25

    申请号：US15025750

    申请日：2014-09-12

    申请人： QIAGEN GMBH

    发明人： Nan Fang

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6851 ,  C12Q2521/107 ,  C12Q2537/101 ,  C12Q2561/113 ,  C12Q2563/173

    摘要： The present invention relates to a method for real-time monitoring and/or quantification of newly-synthesized complementary deoxyribonucleic acid (cDNA) during a reverse transcription reaction of an ribonucleic acid (RNA) template in a sample, the method using a fluorogenic dye binding to RNA:cDNA hybrids. The present invention also relates to the use of this method as well as to kits employing the fluorogenic dye.

    摘要翻译： 本发明涉及在样品中的核糖核酸（RNA）模板的逆转录反应期间实时监测和/或定量新合成的互补脱氧核糖核酸（cDNA）的方法，该方法使用荧光染料结合 RNA：cDNA杂交。 本发明还涉及使用该方法以及使用荧光染料的试剂盒。