公开(公告)号：US06472193B1

公开(公告)日：2002-10-29

申请号：US09600588

申请日：2000-09-11

申请人： Tadaomi Takenawa ,  Masami Hiroyama ,  Tatsuya Kishimoto ,  Masahiro Yamaguchi ,  Mitsuyoshi Toyosato ,  Kouji Mizuno

发明人： Tadaomi Takenawa ,  Masami Hiroyama ,  Tatsuya Kishimoto ,  Masahiro Yamaguchi ,  Mitsuyoshi Toyosato ,  Kouji Mizuno

IPC分类号： C12N914

CPC分类号： C12N9/16 ,  C12Q1/42

摘要： An object of the present invention is to provide a recombinant LPA phosphates capable of specifically hydrolyzing LPA, which is useful for elucidation of the metabolic pathway of LPA, and also for diagnosis and treatment of various diseases with which LPA is associated. The present invention also provides for a method capable of simply and inexpensively determining LPA associated with various diseases. The present invention also provides for a kit for determination suitable for the method. The present invention has succeeded in purifying the LPA phosphatase using bovine brain as a raw material, and further in cloning human LPA phosphatase gene. The present invention specifically relates to a DNA encoding a peptide comprising the amino acid sequence of SEQ ID NO:1; a DNA comprising the nucleotide sequence of SEQ ID NO:2; a protein encoded by the DNA; and expression vector carrying the DNA; a transformant harboring the expression vector; a method for producing a recombinant lysophosphatidic acid phosphatase by the transformant; a method for determination of LPA by the protein; a determination reagent for LPA by the protein; a kit for diagnosis, comprising the reagent for determination, and the like.

摘要翻译： 本发明的目的是提供能够特异性水解LPA的重组LPA磷酸酯，其可用于阐明LPA的代谢途径，以及用于诊断和治疗与LPA相关的各种疾病。 本发明还提供了能够简单且廉价地确定与各种疾病相关的LPA的方法。 本发明还提供了适用于该方法的用于测定的试剂盒。 本发明成功地使用牛脑作为原料来纯化LPA磷酸酶，进一步克隆人类LPA磷酸酶基因。 本发明具体涉及编码包含SEQ ID NO：1的氨基酸序列的肽的DNA; 包含SEQ ID NO：2的核苷酸序列的DNA; 由DNA编码的蛋白质; 和携带DNA的表达载体; 携带表达载体的转化体; 由转化体生产重组溶血磷脂酸性磷酸酶的方法; 一种由蛋白质测定LPA的方法; 用于蛋白质的LPA的测定试剂; 用于诊断的试剂盒，包括用于测定的试剂等。

公开(公告)号：US07109012B2

公开(公告)日：2006-09-19

申请号：US10213100

申请日：2002-08-07

申请人： Tadaomi Takenawa ,  Masami Hiroyama ,  Tatsuya Kishimoto ,  Masahiro Yamaguchi ,  Mitsuyoshi Toyosato ,  Kouji Mizuno

发明人： Tadaomi Takenawa ,  Masami Hiroyama ,  Tatsuya Kishimoto ,  Masahiro Yamaguchi ,  Mitsuyoshi Toyosato ,  Kouji Mizuno

IPC分类号： C12N9/16 ,  C12N15/00 ,  C12N1/20 ,  C12P21/06 ,  C07H21/04

CPC分类号： C12N9/16 ,  C12Q1/42

摘要： An object of the present invention is to provide a recombinant LPA phosphatase capable of specifically hydrolyzing LPA, which is useful for elucidation of the metabolism pathway of LPA, and also for diagnosis and treatment of various diseases with which LPA is associated; a method capable of simply and inexpensively determining LPA associated with various diseases; and a kit for determination suitable for the method. The present invention has succeeded in purifying the LPA phosphatase using bovine brain as raw material, and further in cloning human LPA phosphatase gene. The present invention is accomplished by a DNA encoding a peptide comprising the amino acid sequence of SEQ ID NO:1; a DNA comprising the nucleotide sequence of SEQ ID NO:2; a protein encoded by the DNA; an expression vector carrying the DNA; a transformant harboring the expression vector; a method for producing a recombinant lysophosphatidic acid phosphatase by the transformant; a method for determination of LPA by the protein; a determination reagent for LPA by the protein; a kit for diagnosis, comprising the reagent for determination, and the like.

摘要翻译： 本发明的目的是提供能够特异性水解LPA的重组LPA磷酸酶，其可用于阐明LPA的代谢途径，以及用于诊断和治疗与LPA相关的各种疾病; 能够简单且廉价地确定与各种疾病相关的LPA的方法; 以及适用于该方法的测定用试剂盒。 本发明以牛脑为原料，进一步克隆人LPA磷酸酶基因成功地纯化了LPA磷酸酶。 本发明由编码包含SEQ ID NO：1的氨基酸序列的肽的DNA完成; 包含SEQ ID NO：2的核苷酸序列的DNA; 由DNA编码的蛋白质; 携带DNA的表达载体; 携带表达载体的转化体; 由转化体生产重组溶血磷脂酸性磷酸酶的方法; 一种由蛋白质测定LPA的方法; 用于蛋白质的LPA的测定试剂; 用于诊断的试剂盒，包括用于测定的试剂等。