公开(公告)号：US20130266541A1

公开(公告)日：2013-10-10

申请号：US13441328

申请日：2012-04-06

申请人： Elias T. Zambidis ,  Tea Soon Park

发明人： Elias T. Zambidis ,  Tea Soon Park

IPC分类号： C12N5/10 ,  A61K35/12

CPC分类号： C12N5/0696 ,  A61K35/545 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2506/1369 ,  C12N2510/00

摘要： The present invention relates generally to the field of stem cells and, more particularly, to reprogramming blood cells to pluripotent stem cells. In a specific embodiment, a method for producing an induced pluripotent stem cell from a human myeloid progenitor cell comprising the steps of (a) activating the human myeloid progenitor cell by incubation with hematopoietic growth factors; (b) transfecting the activated progenitor cells with a non-viral vector expressing one or more pluripotency factors; and (c) co-culturing the transfected cells with irradiated mesenchymal bone marrow stromal cells.

摘要翻译： 本发明一般涉及干细胞领域，更具体地涉及将血细胞重编程为多能干细胞。 在一个具体实施方案中，一种从人骨髓祖细胞产生诱导多能干细胞的方法，包括以下步骤：（a）通过与造血生长因子温育来激活人骨髓祖细胞; （b）用表达一种或多种多能因子的非病毒载体转染活化的祖细胞; 和（c）用经照射的间充质骨髓基质细胞共培养转染的细胞。