公开(公告)号：US20120295251A1

公开(公告)日：2012-11-22

申请号：US13514524

申请日：2010-12-09

申请人： Murali Nayudu ,  Andrew Franklin ,  Yafei Zhang ,  Mark John Gibbs ,  Terry John Murphy ,  Adrian John Gibbs ,  Sheba Khan ,  Christian Samundsett

发明人： Murali Nayudu ,  Andrew Franklin ,  Yafei Zhang ,  Mark John Gibbs ,  Terry John Murphy ,  Adrian John Gibbs ,  Sheba Khan ,  Christian Samundsett

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12Q1/70

CPC分类号： C12Q1/6846 ,  C12Q1/686 ,  C12Q2525/179 ,  C12Q2537/143 ,  C12Q2525/15

摘要： The present invention relates to methods for the design and/or production of a probe or primer that is capable of hybridizing to a plurality of sites in a sample comprising nucleic acid. Furthermore, the present invention provides methods for detecting and amplifying nucleic acid using such a probe or primer, for example, for identification of a strain, species or genera. Probe or primer sequences are determined by reference to codon usage bias of a target nucleic acid. In addition, the present invention provides methods for determining codon distribution and/or base pair distance between codons in a nucleic acid.

摘要翻译： 本发明涉及能够与包含核酸的样品中的多个部位杂交的探针或引物的设计和/或生产方法。 此外，本发明提供了使用这种探针或引物检测和扩增核酸的方法，例如用于鉴定菌株，物种或属。 通过参照靶核酸的密码子使用偏差来确定探针或引物序列。 此外，本发明提供了确定核酸中密码子之间的密码子分布和/或碱基对距离的方法。

公开(公告)号：US10081832B2

公开(公告)日：2018-09-25

申请号：US13514524

申请日：2010-12-09

申请人： Murali Nayudu ,  Andrew Franklin ,  Yafei Zhang ,  Mark John Gibbs ,  Terry John Murphy ,  Adrian John Gibbs ,  Sheba Khan ,  Christian Samundsett

发明人： Murali Nayudu ,  Andrew Franklin ,  Yafei Zhang ,  Mark John Gibbs ,  Terry John Murphy ,  Adrian John Gibbs ,  Sheba Khan ,  Christian Samundsett

IPC分类号： C12Q1/6876 ,  C12Q1/6844 ,  C12Q1/686

CPC分类号： C12Q1/6846 ,  C12Q1/686 ,  C12Q2525/179 ,  C12Q2537/143 ,  C12Q2525/15

摘要： The present invention relates to methods for the design and/or production of a probe or primer that is capable of hybridizing to a plurality of sites in a sample comprising nucleic acid. Furthermore, the present invention provides methods for detecting and amplifying nucleic acid using such a probe or primer, for example, for identification of a strain, species or genera. Probe or primer sequences are determined by reference to codon usage bias of a target nucleic acid. In addition, the present invention provides methods for determining codon distribution and/or base pair distance between codons in a nucleic acid.