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    Pressure sensitive adhesive sheet for detection of microorganism and method for detection of microorganism
    1.
    发明授权
    Pressure sensitive adhesive sheet for detection of microorganism and method for detection of microorganism 失效
    用于检测微生物的压敏粘合片和微生物检测方法

    公开(公告)号：US06203900B1

    公开(公告)日：2001-03-20

    申请号：US08885848

    申请日：1997-06-30

    申请人： Takeshi Saika ,  Shuji Senda ,  Yoshitaka Kazuse ,  Akio Iwama ,  Tetsuji Sugii ,  Masao Nasu ,  Katsuji Tani ,  Nobuyasu Yamaguchi

    发明人： Takeshi Saika ,  Shuji Senda ,  Yoshitaka Kazuse ,  Akio Iwama ,  Tetsuji Sugii ,  Masao Nasu ,  Katsuji Tani ,  Nobuyasu Yamaguchi

    IPC分类号： B32B1504

    CPC分类号： C12Q1/04 ,  G01N33/569 ,  G01N33/56916 ,  Y10T428/28 ,  Y10T428/283 ,  Y10T428/2891

    摘要： A pressure sensitive adhesive sheet for the detection of microorganisms, which comprises a laminate of an adhesive layer mainly composed of a water-soluble polymer and a water-permeable membrane which does not allow passage of the microorganisms; a pressure sensitive adhesive sheet for the detection of microorganisms, wherein the surface of the adhesive layer has a contact angle with water of not more than 90°; and a method for detecting a microorganism, which comprises bringing the surface of an adhesive layer of a pressure sensitive adhesive sheet into contact with the surface of a test object, and then bringing the surface of the adhesive layer into contact with water, said adhesive layer or water containing a chromogenic reagent. The use of the laminate makes it possible to leave only stained microorganisms on the water-permeable membrane and to precisely observe the color developed by the microorganisms. The method of the present invention enables real-time monitoring without culture of microorganisms, and is not limited to the detection of viable cells alone. Inasmuch as a contact of the pressure sensitive adhesive sheet with a test face results in accumulation of microorganisms, the manipulation is simple. In addition, by properly determining a chromogenic reagent, a specific microorganism alone can be detected and counted, so that it can be applied to environmental investigation in the medical field, food industry field and the like.

    摘要翻译： 一种用于检测微生物的压敏粘合片，其包括主要由水溶性聚合物组成的粘合剂层和不允许微生物通过的水渗透膜的层压体; 用于检测微生物的压敏粘合片，其中所述粘合剂层的表面与水的接触角不大于90°; 以及检测微生物的方法，其特征在于，使粘合剂层的粘合剂层的表面与试验体的表面接触，然后使粘合剂层的表面与水接触，所述粘合层 或含有显色试剂的水。 使用层压体可以在透水膜上仅留下染色的微生物，并精确地观察由微生物显色的颜色。 本发明的方法能够在不培养微生物的情况下进行实时监测，并不限于单独检测活细胞。 由于压敏粘合片与测试面的接触导致微生物的积累，操作简单。 此外，通过适当地测定显色试剂，可以检测和计数单独的特定微生物，从而可以应用于医疗领域，食品工业领域等的环境调查。