公开(公告)号：US10400031B2

公开(公告)日：2019-09-03

申请号：US15725557

申请日：2017-10-05

申请人： The Govt. of the U.S.A. as rep. by the Secretary of the Dept. of Health and Human Services

发明人： Todd G. Smith ,  Xianfu Wu

IPC分类号： C07K16/10 ,  G01N33/569 ,  C12Q1/00 ,  A61K39/00

摘要： Described herein is a method of identifying a monoclonal antibody (or antigen-binding fragment thereof) that specifically binds a plurality of lyssaviruses for use in post-exposure rabies prophylaxis or in the treatment of clinical rabies. The method includes using a naïve antibody phage display library to screen for phage clones that bind whole recombinant rabies virus or cells expressing glycoprotein from multiple lyssaviruses (such as RABV, MOKV and WCBV) and/or specifically bind recombinant glycoprotein from different lyssaviruses.

公开(公告)号：US20180353593A1

公开(公告)日：2018-12-13

申请号：US16107119

申请日：2018-08-21

申请人： The Govt. of the U.S.A. as rep. by the Secretary of the Dept. of Health and Human Services

发明人： Gwong-Jen J. Chang ,  Wayne D. Crill ,  Holly R. Hughes ,  Brent S. Davis

IPC分类号： A61K39/12 ,  C07K14/005 ,  C12N7/00 ,  A61K39/00

CPC分类号： A61K39/12 ,  A61K2039/5258 ,  A61K2039/53 ,  A61K2039/57 ,  A61K2039/575 ,  A61K2039/6031 ,  A61K2039/6075 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/24122 ,  C12N2770/24123 ,  C12N2770/24134 ,  C12N2770/24151 ,  C12N2770/24171 ,  Y02A50/386

摘要： Described herein are dengue virus E-glycoprotein polypeptides containing mutations that eliminate immunodominant cross-reactive epitopes associated with immune enhancement. The disclosed dengue virus E-glycoproteins optionally further include mutations that introduce a strong CD4 T cell epitope. The disclosed E-glycoprotein polypeptides, or nucleic acid molecules encoding the polypeptides, can be used, for example, in monovalent or tetravalent vaccines against dengue virus.