公开(公告)号：US06720156B2

公开(公告)日：2004-04-13

申请号：US09780718

申请日：2001-02-09

申请人： Jeff T. Hutchins ,  Klaus E. Kuettner ,  Kathryn Mason Lindley ,  Thomas M. Schmid ,  Barbara L. Schumacher ,  Stephen Anthony Stimpson ,  Jui-Lan Su

发明人： Jeff T. Hutchins ,  Klaus E. Kuettner ,  Kathryn Mason Lindley ,  Thomas M. Schmid ,  Barbara L. Schumacher ,  Stephen Anthony Stimpson ,  Jui-Lan Su

IPC分类号： G01N3353

CPC分类号： C07K16/18 ,  A61K2039/53 ,  C12N2799/026 ,  G01N33/6887 ,  G01N2500/00 ,  G01N2800/105 ,  G01N2800/52

摘要： The invention provides an antibody or a fragment thereof having specific binding affinity for superficial zone protein (SZP) or a variant, fragment, or protein core thereof, wherein the binding affinity of the antibody or fragment thereof for human superficial zone protein is the same or greater than the binding affinity for bovine superficial zone protein in a competitive binding assay, IAsys analysis, or BIAcore analysis. The present invention further provides hybidoma cells that produce the monoclonal antibody and antibody reagent kits comprising the antibody or fragment of the invention. Further provided by the invention are methods of SZP detection, methods of diagnosing a degenerative joint condition, and screening methods related to the use of the antibody or fragment thereof.

摘要翻译： 本发明提供对表浅区蛋白（SZP）或其变体，片段或蛋白核心具有特异性结合亲和力的抗体或其片段，其中抗体或其片段对人表皮区蛋白的结合亲和力相同或 在竞争性结合测定，IAsys分析或BIAcore分析中大于牛表面区蛋白的结合亲和力。 本发明还提供产生单克隆抗体的杂交瘤细胞和包含本发明的抗体或片段的抗体试剂盒。 本发明还提供了SZP检测方法，诊断退行性关节病的方法以及与抗体或其片段的使用相关的筛选方法。