公开(公告)号：US06753421B2

公开(公告)日：2004-06-22

申请号：US09544934

申请日：2000-04-07

Applicant: Henrik Stender ,  Kaare Lund ,  Tina Anderson Mollerup

Inventor： Henrik Stender ,  Kaare Lund ,  Tina Anderson Mollerup

IPC: C07H2102

CPC classification number: C07K14/003 ,  C12Q1/689

Abstract: Novel hybridisation assay probes and mixtures of such probes for detecting a target sequence of one or mycobacteria optionally present in a sample. The probes may suitable be directed to target sequences of mycobacterial rDNA, precursor rRNA or rRNA, said probes being capable of forming detectable hybrids. The probes are in particular directed to mycobacterial rDNA, to precursor rRNA, or to 23S, 16S or 5S rRNA. The probes are useful for detecting the organisms in test samples such as sputum, laryngeal swabs, gastric lavage, bronchial washings, biopsies, aspirates, expectorates, body fluids (spinal, pleural, pericardial synovial, blood, pus, bone marrow), urine, tissue sections as well as food samples, soil, air and water samples, and cultures thereof.

Abstract translation: 新型杂交测定探针和这种探针的混合物，用于检测任选存在于样品中的一种或分枝杆菌的靶序列。 探针可以适用于分枝杆菌rDNA，前体rRNA或rRNA的靶序列，所述探针能够形成可检测的杂交体。 探针特别针对分枝杆菌rDNA，前体rRNA或23S，16S或5S rRNA。 探针可用于检测试验样品中的生物，如痰液，喉拭子，胃灌洗液，支气管洗液，活组织检查，抽吸物，痰液，体液（脊髓，胸膜，心包滑膜，血液，脓液，骨髓），尿液， 组织切片以及食物样品，土壤，空气和水样品及其培养物。