公开(公告)号：US08357782B2

公开(公告)日：2013-01-22

申请号：US12933768

申请日：2009-03-16

申请人： Shigeharu Fukuda ,  Chie Ushio ,  Harumi Ariyasu ,  Tohru Kayano ,  Toshio Ariyasu ,  Tsunetaka Ohta

发明人： Shigeharu Fukuda ,  Chie Ushio ,  Harumi Ariyasu ,  Tohru Kayano ,  Toshio Ariyasu ,  Tsunetaka Ohta

IPC分类号： C07K16/24 ,  A61K39/395 ,  C12N5/12

CPC分类号： C07K16/249 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/76 ,  G01N33/6866 ,  G01N2030/8831

摘要： The present invention has the first object to provide a monoclonal antibody specific to interferon α subtype α8 (IFNα8) and its mutant proteins, the second object to provide a hybridoma capable of producing the monoclonal antibody, the third object to provide a method for detecting the IFNα8 and its mutant proteins by the monoclonal antibody, the fourth object to provide a method for purifying the IFNα8 and its mutant proteins by the monoclonal antibody, and the fifth object to provide a therapeutic agent for treating diseases whose onsets or exacerbation are related with IFNα8. The present invention solves the above objects by providing a monoclonal antibody specific to IFNα8 and its mutant proteins, a hybridoma capable of producing the monoclonal antibody, a method for detecting the IFNα8 and its mutant proteins by immunoreaction using the monoclonal antibody, a method for purifying the IFNα8 and its mutant proteins using the monoclonal antibody, and a therapeutic agent for treating diseases whose onsets or exacerbation are related with IFNα8, which contains the monoclonal antibody as an effective ingredient.

摘要翻译： 本发明的第一个目的是提供对干扰素α亚型α8（IFNα8）及其突变蛋白特异性的单克隆抗体，第二个目的是提供能产生单克隆抗体的杂交瘤，第三个目的是提供一种检测方法 通过单克隆抗体IFNα8及其突变蛋白，第四个目的是提供通过单克隆抗体纯化IFNα8及其突变蛋白的方法，第五个目的是提供一种用于治疗其发作或加重与IFNα8相关的疾病的治疗剂 。 本发明通过提供对IFNα8及其突变蛋白特异性的单克隆抗体，能够产生单克隆抗体的杂交瘤，通过使用单克隆抗体的免疫反应检测IFNα8及其突变蛋白的方法来解决上述目的，纯化方法 使用单克隆抗体的IFNα8及其突变蛋白，以及用于治疗其发作或恶化与IFNα8相关的疾病的治疗剂，其含有单克隆抗体作为有效成分。

公开(公告)号：US20110091968A1

公开(公告)日：2011-04-21

申请号：US12933768

申请日：2009-03-16

申请人： Shigeharu Fukuda ,  Chie Ushio ,  Harumi Ariyasu ,  Tohru Kayano ,  Toshio Ariyasu ,  Tsunetaka Ohta

发明人： Shigeharu Fukuda ,  Chie Ushio ,  Harumi Ariyasu ,  Tohru Kayano ,  Toshio Ariyasu ,  Tsunetaka Ohta

IPC分类号： C07K16/24 ,  G01N33/566 ,  C07K1/22 ,  C12N5/16

CPC分类号： C07K16/249 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/76 ,  G01N33/6866 ,  G01N2030/8831

摘要： The present invention has the first object to provide a monoclonal antibody specific to interferon α subtype α8 (IFNα8) and its mutant proteins, the second object to provide a hybridoma capable of producing the monoclonal antibody, the third object to provide a method for detecting the IFNα8 and its mutant proteins by the monoclonal antibody, the fourth object to provide a method for purifying the IFNα8 and its mutant proteins by the monoclonal antibody, and the fifth object to provide a therapeutic agent for treating diseases whose onsets or exacerbation are related with IFNα8. The present invention solves the above objects by providing a monoclonal antibody specific to IFNα8 and its mutant proteins, a hybridoma capable of producing the monoclonal antibody, a method for detecting the IFNα8 and its mutant proteins by immunoreaction using the monoclonal antibody, a method for purifying the IFNα8 and its mutant proteins using the monoclonal antibody, and a therapeutic agent for treating diseases whose onsets or exacerbation are related with IFNα8, which contains the monoclonal antibody as an effective ingredient.

摘要翻译： 本发明的第一个目的是提供对干扰素α亚型α8（IFNα8）及其突变蛋白特异性的单克隆抗体，第二个目的是提供能产生单克隆抗体的杂交瘤，第三个目的是提供一种检测方法 通过单克隆抗体IFNα8及其突变蛋白，第四个目的是提供通过单克隆抗体纯化IFNα8及其突变蛋白的方法，第五个目的是提供一种用于治疗其发作或加重与IFNα8相关的疾病的治疗剂 。 本发明通过提供对IFNα8及其突变蛋白特异性的单克隆抗体，能够产生单克隆抗体的杂交瘤，通过使用单克隆抗体的免疫反应检测IFNα8及其突变蛋白的方法来解决上述目的，纯化方法 使用单克隆抗体的IFNα8及其突变蛋白，以及用于治疗其发作或恶化与IFNα8相关的疾病的治疗剂，其含有单克隆抗体作为有效成分。

公开(公告)号：US20060073519A1

公开(公告)日：2006-04-06

申请号：US11224106

申请日：2005-09-13

申请人： Tohru Kayano ,  Mutsuko Taniguchi ,  Hiroshi Yamauchi ,  Masashi Kurimoto

发明人： Tohru Kayano ,  Mutsuko Taniguchi ,  Hiroshi Yamauchi ,  Masashi Kurimoto

IPC分类号： G01N33/53 ,  A61K39/395

CPC分类号： C07K16/244 ,  A61K2039/505 ,  Y02A50/412 ,  Y02A50/58

摘要： Disclosed are an antibody specific to interleukin 18 (IL-18) precursor, preparation processes therefor, and uses thereof. The antibody includes immunoglobulins in both forms of polyclonal and monoclonal antibodies which exhibits an immunoreactivity against IL-18 precursor at an intensity higher than against other substances. The antibody is useful in detection and purification of IL-18 precursor and in elimination and detoxification of the precursor accumulated in vivo. The detection method using the antibody is effective in qualitative and quantitative analyses for the precursor as well as in correction of imprecise results caused by assays using anti-IL-18 antibody which exhibits a cross reactivity against the precursor.

摘要翻译： 公开了对白细胞介素18（IL-18）前体特异性的抗体，其制备方法及其应用。 抗体包括两种形式的多克隆和单克隆抗体的免疫球蛋白，其以比其它物质高的强度显示出对IL-18前体的免疫反应性。 该抗体可用于检测和纯化IL-18前体以及在体内积累的前体的消除和解毒。 使用抗体的检测方法对于前体的定性和定量分析以及校正由对前体具有交叉反应性的抗IL-18抗体的测定引起的不精确结果是有效的。