公开(公告)号：US4710461A

公开(公告)日：1987-12-01

申请号：US642191

申请日：1984-08-20

申请人： Tohru Komano ,  Kanji Ohyma

发明人： Tohru Komano ,  Kanji Ohyma

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/70 ,  C12N15/82 ,  C12P21/00 ,  C12N1/00 ,  C12N15/00

CPC分类号： C12N15/70 ,  C12N15/8216

摘要： A promoter comprises a DNA having the same base sequence as that of a DNA fraction having a size of about 1.1 kbp, which is obtained by cleavage by restriction enzyme Bam HI of a DNA fraction comprising all of a 16SrRNA gene and a part of a 23SrRNA gene, said DNA fraction being obtained by cleavage by restriction enzyme Eco RI of chloroplast DNA of Marchantia polymorpha L. The promoter is integrated with a DNA having the same sequence as that of DNA derived from a procaryote into a vector.

摘要翻译： 启动子包含与具有约1.1kbp大小的DNA级分相同的碱基序列的DNA，其通过用限制酶Bam HI切割包含所有16SrRNA基因和部分23SrRNA的DNA部分获得 基因，所述DNA部分是通过限制酶EcoRI切割多形汉逊酵母的叶绿体DNA得到的。该启动子与具有与源自原核生物的DNA相同序列的DNA整合到载体中。

公开(公告)号：US5399483A

公开(公告)日：1995-03-21

申请号：US978442

申请日：1992-11-18

申请人： Yuji Shibano ,  Kazumitsu Ueda ,  Tohru Komano

发明人： Yuji Shibano ,  Kazumitsu Ueda ,  Tohru Komano

IPC分类号： C07K14/705 ,  C12N1/19 ,  C12N15/81 ,  C12Q1/02

CPC分类号： C07K14/705 ,  C12N15/81 ,  C12Q1/02

摘要： A yeast host which can express P-glycoprotein, i.e., the product of MDR-related gene, in the cell membrane in the same state as observed in multidrug resistant cells produced by connecting the MDR-related gene which carries multidrug resistance to a yeast expression vector and transforming the yeast with said recombinant vector; a cell membrane fraction containing a substantial amount of P-glycoprotein produced by said yeast and a process for the preparation thereof; and a recombinant vector for expressing the MDR-related gene in a yeast host.

摘要翻译： 可以在与将多药耐药性相关的MDR相关基因连接到酵母表达产生的多药耐药细胞中观察到的同样状态下能够表达P-糖蛋白即MDR相关基因的产物的酵母宿主在细胞膜中 载体并用所述重组载体转化酵母; 含有由所述酵母产生的大量P-糖蛋白的细胞膜级分及其制备方法; 以及用于在酵母宿主中表达MDR相关基因的重组载体。