公开(公告)号：US4698142A

公开(公告)日：1987-10-06

申请号：US760940

申请日：1985-07-31

申请人： Katsumi Muroi ,  Kosai Hiratsuka ,  Shoji Yoshinaga ,  Toji Nakatsui ,  Chikao Oda ,  Kiyoshi Fujiwara

发明人： Katsumi Muroi ,  Kosai Hiratsuka ,  Shoji Yoshinaga ,  Toji Nakatsui ,  Chikao Oda ,  Kiyoshi Fujiwara

IPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/28 ,  G01N27/26

CPC分类号： G01N27/44769 ,  B01D57/02

摘要： The invention discloses a free-flow electrophoretic separation method and apparatus for separating charged substances such as proteins, cells, and the like, by electrophoresis, which comprises supplying a mixed solution containing the charged substances to be separated, which are dissolved in a separation buffer, into a separation chamber, circulating the mixed solution inside the separation chamber, applying a d.c. voltage to the mixed solution to cause the electrophoresis of the charged substances and to separate them, and withdrawing the charged substances thus separated from outlets disposed at one and the other end of the separation chamber.

摘要翻译： 本发明公开了一种通过电泳分离带电物质如蛋白质，细胞等的自由流动电泳分离方法和装置，其包括将含有待分离的带电物质的混合溶液加入到分离缓冲液 进入分离室，使分离室内的混合溶液循环，施加直流 向混合溶液施加电压以引起带电物质的电泳并将其分离，并且从这些与分离室一端和另一端的出口分离的带电物质取出。

公开(公告)号：US4749458A

公开(公告)日：1988-06-07

申请号：US020773

申请日：1987-03-02

申请人： Katsumi Muroi ,  Kosai Hiratsuka ,  Shoji Yoshinaga ,  Toji Nakatsui ,  Chikao Oda ,  Kiyoshi Fujiwara

发明人： Katsumi Muroi ,  Kosai Hiratsuka ,  Shoji Yoshinaga ,  Toji Nakatsui ,  Chikao Oda ,  Kiyoshi Fujiwara

IPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/28 ,  G01N27/26

CPC分类号： G01N27/44769 ,  B01D57/02

摘要： The invention discloses a free-flow electrophoretic separation method and apparatus for separating charged substances such as proteins, cells, and the like, by electrophoresis, which comprises supplying a mixed solution containing the charged substances to be separated, which are dissolved in a separation buffer, into a separation chamber, circulating the mixed solution inside the separation chamber, applying a d.c. voltage to the mixed solution to cause the electrophoresis of the charged substances and to separate them, and withdrawing the charged substances thus separated from outlets disposed at one and the other end of the separation chamber.