公开(公告)号：US20240319136A1

公开(公告)日：2024-09-26

申请号：US18615006

申请日：2024-03-25

申请人： Matthew Ryan COURTNEY ,  Tomasz GLAWDEL ,  Carolyn Liqing REN ,  Oscar Manuel CARRENO-MOLINA

发明人： Matthew Ryan COURTNEY ,  Tomasz GLAWDEL ,  Carolyn Liqing REN ,  Oscar Manuel CARRENO-MOLINA

IPC分类号： G01N27/447 ,  G01N27/416

CPC分类号： G01N27/44769 ,  G01N27/4167

摘要： A free-flow electrophoresis apparatus introduces a pressure-driven flow laterally through the sidewalls of a separation chamber. A neutral solute is hydrodynamically focused by the lateral flow before being collected through an outlet at a bottom of the chamber. Meanwhile, charged solutes are removed from the neutral solute stream by application of an electric field. As a result, the neutral solute is both concentrated and isolated from a complex mixture. This purification technique is also versatile, as a pH of the separation medium may be chosen to match a pI of the desired solute.

公开(公告)号：US20240142434A1

公开(公告)日：2024-05-02

申请号：US18383330

申请日：2023-10-24

申请人： ARKRAY, Inc.

发明人： Misaki OGA ,  Naotsugu ONUMA

IPC分类号： G01N33/49 ,  G01N27/447

CPC分类号： G01N33/491 ,  G01N27/44747 ,  G01N27/44769 ,  G01N2333/4713 ,  G01N2333/76

摘要： Provided are a sample analysis method including separating albumin and γ-globulin from a sample containing albumin and γ-globulin, in an alkaline solution by capillary electrophoresis, in which the alkaline solution contains a cationic polymer, and the like.

公开(公告)号：US20230158504A1

公开(公告)日：2023-05-25

申请号：US18159284

申请日：2023-01-25

申请人： UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION

发明人： HONGYAO GENG ,  SUNG KWON CHO

IPC分类号： B01L3/00 ,  G01N27/06 ,  G01N27/447

CPC分类号： B01L3/502792 ,  G01N27/06 ,  G01N27/44769 ,  B01L2300/161 ,  B01L2400/0427

摘要： A digital microfluidic system includes a substrate, a plurality of electrode sets provided on the substrate, wherein each of the electrode sets includes two co-planar interdigitated finger electrodes, and a driving circuit including an AC/DC voltage source and a controller. Each of the electrode sets is individually addressable by the driving circuit under control of the controller such that an AC/DC voltage generated by the AC/DC voltage source may be selectively provided to one or more of the electrode sets. Also, an anti-biofouling electrode for a digital microfluidic system includes an electrode layer, and a slippery liquid infused porous surface structure provided on the electrode layer.

公开(公告)号：US20180158664A1

公开(公告)日：2018-06-07

申请号：US15832537

申请日：2017-12-05

申请人： Beijing Institute Of Technology

发明人： Wei Xu ,  Muyi He ,  Pan Luo

IPC分类号： H01J49/16 ,  G01N27/447 ,  G01N30/72 ,  G01N33/68 ,  G01N30/46 ,  G01N27/62 ,  H01J49/04

CPC分类号： H01J49/167 ,  G01N27/44765 ,  G01N27/44769 ,  G01N27/44773 ,  G01N27/622 ,  G01N30/463 ,  G01N30/7266 ,  G01N33/68 ,  H01J49/0404 ,  G01N27/4473

摘要： The present invention provides a mobility electrophoresis separation device, its operating method, and an interface between liquid chromatography and mass spectrometry. The mobility electrophoresis separation device comprises a separation capillary, a syringe pump for injecting a buffer solution, a syringe for injecting a sample solution, and two electrodes disposed apart from each other on either side of the separation capillary. A sample solution is injected by a syringe at a position of the capillary channel, and a buffer solution is injected into the capillary channel upstream the first position, and carries the sample solution to flow downstream. While the mixed liquid flows through the capillary, an electric field is applied in the direction of the flow. Different ions in the sample are thus separated in the flow due to their different velocities traveling in the flow.

公开(公告)号：US20170326524A1

公开(公告)日：2017-11-16

申请号：US15527836

申请日：2015-12-18

申请人： SHARP MICROFLUIDIC SOLUTIONS LIMITED

发明人： Benjamin James HADWEN ,  Christopher James BROWN

IPC分类号： B01J19/00 ,  G01N27/447 ,  B01L3/00 ,  H01L21/67

CPC分类号： B01J19/004 ,  B01L3/50273 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2400/0424 ,  B01L2400/0427 ,  F04B19/006 ,  G01N27/44769 ,  G02B26/005 ,  G09G3/348 ,  G09G2230/00 ,  G09G2300/0842 ,  G09G2300/0852 ,  G09G2300/0857 ,  G09G2310/0251 ,  H01L21/67023 ,  H01L21/67132

摘要： A method of driving an element of an active matrix electro-wetting on dielectric (AM-EWOD) device comprise applying a first alternating voltage to a reference electrode of the AM-EWOD device; and either (i) applying to the element electrode a second alternating voltage that has the same frequency as the first alternating voltage and that is out of phase with the first alternating voltage or (ii) holding the element electrode in a high impedance state. The effect of applying the second alternating voltage to the element electrode is to put the element in an actuated state in which the element is configured to actuate any liquid droplet present in the element, while the effect of holding the element electrode in the high impedance state is to put the element in a non-actuated state.

公开(公告)号：US20160238560A1

公开(公告)日：2016-08-18

申请号：US14902402

申请日：2014-07-04

申请人： FFE SERVICE GMBH

发明人： Christoph Weber ,  Gerhard Weber

IPC分类号： G01N27/447

CPC分类号： G01N27/44795 ,  G01N27/44769

摘要： The present invention is related to a free-flow electrophoresis method for separating at least one analyte of interest from a mixture of analytes, wherein the method uses a separation medium comprising two or more individual separation media, wherein the two or more individual separation media differ in their pH value, and wherein each of the two or more individual separation media comprise at least one anion of at least one acid and at least one cation of at least one base, wherein the at least one acid is the same in each of the two or more individual separation media and the at least one base is the same in each of the two or more individual separation media.

摘要翻译： 本发明涉及一种用于从分析物混合物中分离出至少一种感兴趣的分析物的自由流动电泳方法，其中该方法使用包含两种或多种单独分离介质的分离介质，其中两种或多种单独的分离介质不同 在其pH值中，并且其中两个或更多个单独的分离介质中的每一个包含至少一种酸的至少一种阴离子和至少一种碱的至少一种阳离子，其中所述至少一种酸在每种 两个或更多个单独的分离介质和至少一个碱基在两个或多个单独的分离介质中的每一个中相同。

公开(公告)号：US20160097087A1

公开(公告)日：2016-04-07

申请号：US14844279

申请日：2015-09-03

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Willy Wiyatno ,  Linda Lee ,  David Cox ,  James Nurse ,  Douglas Greiner ,  Dennis Lehto ,  Janusz Wojtowicz ,  Sam Woo ,  Richard Reel

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  B01J2219/00353 ,  B01J2219/0036 ,  B01J2219/00364 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/0293 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0421 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q2535/101 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0011 ,  F16K99/0013 ,  F16K2099/0084 ,  G01N1/14 ,  G01N27/44743 ,  G01N27/44769 ,  G01N35/08 ,  Y10T137/0318 ,  Y10T137/4259 ,  Y10T137/85978 ,  Y10T137/85986 ,  Y10T137/86863 ,  Y10T436/2575

摘要： Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

摘要翻译： 在本申请中描述的各种实施例涉及用于在管道内产生与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。 离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。 该系统可以包括一种装置，该装置包括至少一个导管，其操作地连接到一个或多个泵，用于提供通过与离散体积的流体不混溶的流体彼此分离的离散体积，例如水不混溶流体 - 油分离体积。

公开(公告)号：US20150153308A1

公开(公告)日：2015-06-04

申请号：US14555793

申请日：2014-11-28

申请人： TECHNION RESEARCH AND DEVELOPMENT FOUNDATION LIMITED

发明人： Moran Bercovici ,  Ortal Schwartz

IPC分类号： G01N27/447

CPC分类号： G01N27/44769 ,  B01L3/50273 ,  B01L2200/0652 ,  B01L2300/06 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0487 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44743 ,  G01N27/44756 ,  G01N27/44765 ,  G01N27/44791 ,  G01N33/561 ,  G01N33/56911

摘要： The present invention provides a system including: a protein having a domain that binds a membranal component; an inlet for sample flow, an Isotachophoresis (ITP) system and a flow generating means connected or coupled to the aqueous parts of the ITP. The invention also provides a method for detecting and or sorting cells with this system.

摘要翻译： 本发明提供一种系统，其包括：具有结合膜组分的结构域的蛋白质; 用于样品流的入口，等速电泳（ITP）系统和连接或耦合到ITP的水性部分的流动产生装置。 本发明还提供了一种用该系统检测和分类细胞的方法。

公开(公告)号：US08388909B2

公开(公告)日：2013-03-05

申请号：US12576794

申请日：2009-10-09

申请人： Michael G Pollack ,  Vamsee K Pamula ,  Richard B Fair

发明人： Michael G Pollack ,  Vamsee K Pamula ,  Richard B Fair

IPC分类号： B01L3/00

CPC分类号： G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

摘要： Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

公开(公告)号：US08349276B2

公开(公告)日：2013-01-08

申请号：US11343284

申请日：2006-01-30

申请人： Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

发明人： Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

IPC分类号： B01L3/00

CPC分类号： G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

摘要： Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.

摘要翻译： 公开了在印刷电路板（PCB）上操纵液滴的装置和方法。 通过将电位施加到PCB上限定的电极，使液滴在印刷电路板基板表面上被致动。 还公开了使用焊接掩模作为液滴操作的电极绝缘体以及用于适应其它传统PCB层和用于液滴的微流体的材料的技术。