公开(公告)号：US4909919A

公开(公告)日：1990-03-20

申请号：US274984

申请日：1988-11-22

申请人： Michael D. Morris ,  Chang-Yuh Chen ,  Tshengedzeni Demana ,  Shi-Duo G. Huang

发明人： Michael D. Morris ,  Chang-Yuh Chen ,  Tshengedzeni Demana ,  Shi-Duo G. Huang

IPC分类号： G01N27/447

CPC分类号： G01N27/4473 ,  G01N27/44773

摘要： A capillary zone electrophoresis system with analyte velocity modulation is described. Velocity modulation of the analyte is provided through applying an AC component to the DC driving potential applied to the separation capillary. Typically modulation on the order of 20 to 30% is applied by a separate AC power supply or an integral DC/AC source. Numerous types of detector systems can be employed including refractive index detectors, conductance, fluorescence, etc. The output of the detector is demodulated using a synchronous demodulation. The velocity modulation generates a signal which is a derivative of the conventional concentration/time signal. The system vastly improves detector sensitivity in situations where limitations are imposed by the large and variable background signals which arise from the aqueous buffer itself. Numerous embodiments of means for applying the modulated driving potential are described along with several detectors which can be implemented in systems according to this invention.

摘要翻译： 描述了具有分析物速度调制的毛细管区带电泳系统。 通过将AC分量施加到施加到分离毛细管的DC驱动电位来提供分析物的速度调制。 通常，通过单独的AC电源或集成DC / AC电源施加大约20至30％的调制。 可以使用多种类型的检测器系统，包括折射率检测器，电导，荧光等。检测器的输出使用同步解调进行解调。 速度调制产生作为常规浓度/时间信号的导数的信号。 在由水性缓冲液本身产生的大的和可变的背景信号施加限制的情况下，该系统极大地提高了检测器灵敏度。 与应用本发明的系统中可实施的几个检测器一起描述用于施加调制的驱动电位的装置的许多实施例。