公开(公告)号：US20090292479A1

公开(公告)日：2009-11-26

申请号：US12126599

申请日：2008-05-23

Applicant: Tsung-kai Chuang ,  Tzu-chiang Wu ,  Jiann-hua Wang

Inventor： Tsung-kai Chuang ,  Tzu-chiang Wu ,  Jiann-hua Wang

IPC: G06F19/00

CPC classification number: G01N21/6452 ,  G06T7/73 ,  G06T2207/30024

Abstract: A method for analyzing an image from a bio-detection analyzer includes steps of: illuminating a reacted bio-detection carrier having at least one detection spot and at least one positioning spot by at least one light source; capturing a single image of the bio-detection carrier having all of detection-spot images and positioning-spot images by an image capturing unit; defining a reference coordinate location of each of the detection-spot images according to the positioning-spot image; defining an effective detection area of each of the detection-spot images according to the reference coordinate location of each of the detection-spot images; and analyzing an averaged intensity value (such as an averaged gray scale value) of each of the detection-spot images, so as to output the averaged intensity value for showing a reaction result of the reacted bio-detection carrier.

Abstract translation: 用于从生物检测分析仪分析图像的方法包括以下步骤：通过至少一个光源照射具有至少一个检测点和至少一个定位斑点的反应的生物检测载体; 通过图像捕获单元捕获具有所有检测点图像和定位图像的生物检测载体的单个图像; 根据定位点图像定义每个检测点图像的参考坐标位置; 根据每个检测点图像的参考坐标位置来定义每个检测点图像的有效检测区域; 并分析每个检测点图像的平均强度值（诸如平均灰度值），以输出用于显示反应的生物检测载体的反应结果的平均强度值。

公开(公告)号：US20090058617A1

公开(公告)日：2009-03-05

申请号：US11964152

申请日：2007-12-26

Applicant: Tzu-Chiang Wu ,  Chein-Ho Chuang ,  Tsung-Kai Chuang ,  Jiann-Hua Wang

Inventor： Tzu-Chiang Wu ,  Chein-Ho Chuang ,  Tsung-Kai Chuang ,  Jiann-Hua Wang

IPC: H04Q5/22

CPC classification number: G01N35/00732 ,  G01N2035/00851

Abstract: A method for setting up a system of a reagent chip analyzer is provided. The method is adapted to modify parameters and testing conditions according to data of an authorization tag, so as to improve the adaptability range of the reagent chip analyzer for reagent chips of multiple specifications. The method includes: reading an identification code, an authorization times, and specifications and analysis parameters of the reagent chip from the RFID tag; determining whether to load the specifications and the analysis parameters of the reagent chip according to the identification code and the authorization times; and if it is determined to load the specifications and the analysis parameters of the reagent chip then decreasing the authorization times by degrees, and analyzing the reagent chip according to the specifications and the analysis parameters of the reagent chip.

Abstract translation: 提供了一种用于设置试剂芯片分析仪的系统的方法。 该方法适用于根据授权标签的数据修改参数和测试条件，以提高多种规格的试剂芯片的试剂芯片分析仪的适应范围。 该方法包括：从RFID标签读取试剂芯片的识别码，授权时间，规格和分析参数; 根据识别码和授权时间确定是否加载试剂芯片的规格和分析参数; 如果确定加载试剂芯片的规格和分析参数，然后减小授权次数，并根据试剂芯片的规格和分析参数分析试剂芯片。

公开(公告)号：USD580559S1

公开(公告)日：2008-11-11

申请号：US29293339

申请日：2007-11-17

Applicant: Jin-Po Lee ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Jiann-Hua Wang

Designer： Jin-Po Lee ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Jiann-Hua Wang

公开(公告)号：US08195408B2

公开(公告)日：2012-06-05

申请号：US11964152

申请日：2007-12-26

Applicant: Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang ,  Jiann-Hua Wang

Inventor： Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang ,  Jiann-Hua Wang

IPC: G01N31/00

CPC classification number: G01N35/00732 ,  G01N2035/00851

Abstract: A method for setting up a system of a reagent chip analyzer is provided. The method is adapted to modify parameters and testing conditions according to data of an authorization tag, so as to improve the adaptability range of the reagent chip analyzer for reagent chips of multiple specifications. The method includes: reading an identification code, authorization times, and specifications and analysis parameters of the reagent chip from an RFID tag; determining whether to load the specifications and the analysis parameters of the reagent chip according to the identification code and the authorization times; and if it is determined to load the specifications and the analysis parameters of the reagent chip, decreasing the authorization times by one, and analyzing the reagent chip according to the specifications and the analysis parameters of the reagent chip.

Abstract translation: 提供了一种用于设置试剂芯片分析仪的系统的方法。 该方法适用于根据授权标签的数据修改参数和测试条件，以提高多种规格的试剂芯片的试剂芯片分析仪的适应范围。 该方法包括：从RFID标签读取试剂芯片的识别码，授权时间，规格和分析参数; 根据识别码和授权时间确定是否加载试剂芯片的规格和分析参数; 如果确定加载试剂芯片的规格和分析参数，则将授权时间减少一次，并根据试剂芯片的规格和分析参数对试剂芯片进行分析。

公开(公告)号：US20050233376A1

公开(公告)日：2005-10-20

申请号：US11166238

申请日：2005-06-27

Applicant: Jiann-Hua Wang ,  Hui-Ju Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

Inventor： Jiann-Hua Wang ,  Hui-Ju Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

IPC: C12M1/34 ,  C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/6452 ,  G01N21/6456

Abstract: A biochip scanner device includes a light source for emitting a light beam; a light processing unit for focusing the light beam onto the biochip to excite fluorescence from a sample spot on the biochip; a filter for filtering off the light beam from the light source and the scattered light from the surface on the biochip; a photomultiplier tube (PMT) for detecting and converting the fluorescence into a current signal; and an output device for outputting/displaying the current signal detected by the PMT. The output device controls the platform to match the pattern of the biochip. No conversion of the output signal of the output device into image data is needed. A real-time analysis proceeds while samples are being scanned on the biochip. The biochip scanner device of the present invention reads the current signal from PMT directly without processing it into image data and setting lens before the PMT is no longer needed. As a result, the structure of the device is simplified and the cost for production is reduced.

Abstract translation: 生物芯片扫描器装置包括用于发射光束的光源; 光处理单元，用于将光束聚焦到生物芯片上以从生物芯片上的样品点激发荧光; 用于滤除来自光源的光束的滤光器和来自生物芯片上的表面的散射光; 用于检测并将荧光转换成电流信号的光电倍增管（PMT）; 以及用于输出/显示由PMT检测的电流信号的输出装置。 输出设备控制平台以匹配生物芯片的图案。 不需要将输出设备的输出信号转换为图像数据。 在生物芯片上扫描样品时，进行实时分析。 本发明的生物芯片扫描器装置在PMT不再需要之前直接从PMT读取当前信号而不将其处理成图像数据和设置透镜。 结果，简化了装置的结构，降低了生产成本。

公开(公告)号：US20050179900A1

公开(公告)日：2005-08-18

申请号：US10777651

申请日：2004-02-13

Applicant: Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

Inventor： Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

IPC: G01N21/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6456

Abstract: A CCD-based biochip reader includes a light source for emitting light beams, a collimating lens for converting the light beams into wide parallel rays of light, passing said wide parallel rays of light through a biochip and exciting fluorescence from fluorescent targets on the biochip, a focusing lens for focusing the fluorescence, a filter for filtering out said parallel rays of light, and a charge-coupled device camera for generating images from said fluorescence. For recording intensity of the fluorescence from the fluorescent targets, images of the charge-coupled device camera is converted into digital data through an image converting device. Wide parallel laser beams are produced by the laser and through the collimating lens, and excite all samples on the biochip with high efficiency at the same time. In addition, time for analysis is saved when fluorescent images of a large area on biochips are collected and analyzed through the charge-coupled device camera.

Abstract translation: 基于CCD的生物芯片读取器包括用于发射光束的光源，用于将光束转换成宽平行光线的准直透镜，使所述宽平行光线通过生物芯片并从生物芯片上的荧光目标激发荧光， 用于聚焦荧光的聚焦透镜，用于滤出所述平行光线的滤光器和用于从所述荧光产生图像的电荷耦合器件相机。 为了记录来自荧光目标的荧光的强度，电荷耦合器件相机的图像通过图像转换装置转换成数字数据。 通过激光和准直透镜产生宽平行激光束，同时以高效率激发生物芯片上的所有样品。 此外，通过电荷耦合器件相机收集和分析生物芯片上大面积的荧光图像时，可节省分析时间。

公开(公告)号：US20080085213A1

公开(公告)日：2008-04-10

申请号：US11542179

申请日：2006-10-04

Applicant: Tzu-Chiang Wu ,  Mao-Ji Lin ,  Chien-Ho Chuang ,  Tsung-Kai Chuang ,  Jiann-Hua Wang

Inventor： Tzu-Chiang Wu ,  Mao-Ji Lin ,  Chien-Ho Chuang ,  Tsung-Kai Chuang ,  Jiann-Hua Wang

IPC: G01N33/48

CPC classification number: G01N33/48714

Abstract: A cartridge-type reagent chip analyzer is provided to analyze various specifications of reagent chips. A primary feature of the present invention is to provide a gear mounted on a base and two gear racks symmetrically meshing with the gear. The gear racks are connected to two guiding components and connected to the base by two springs respectively. When a reagent chip is inserted, the guiding components are pushed apart and guide the reagent chip to enter the analyzer. When the reagent chip is ejected out of the analyzer, the springs force the guiding components back to their original positions.

Abstract translation: 提供了一种盒型试剂芯片分析仪，用于分析试剂芯片的各种规格。 本发明的主要特征在于提供安装在基座上的齿轮和与齿轮对称地啮合的两个齿轮架。 齿轮架连接到两个引导部件，并分别由两个弹簧连接到基座。 当插入试剂芯片时，引导部件被推开并引导试剂芯片进入分析器。 当试剂芯片从分析仪中排出时，弹簧迫使导向部件回到原来的位置。

公开(公告)号：US20050157300A1

公开(公告)日：2005-07-21

申请号：US10760465

申请日：2004-01-21

Applicant: Jiann-Hua Wang ,  Hui Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

Inventor： Jiann-Hua Wang ,  Hui Chen ,  Tzu-Chiang Wu ,  Chien-Ho Chuang ,  Tsung-Kai Chuang

IPC: C12M1/34 ,  C12Q1/68 ,  G01N21/64

CPC classification number: G01N21/6456 ,  G01N21/6428 ,  G01N21/6452

Abstract: A biochip scanner device includes a light source for emitting a light beam; a light processing unit for focusing the light beam onto the biochip to excite fluorescence from a fluorescent target on the biochip; a filter for filtering off the light beam from the light source; a photomultiplier tube (PMT) for detecting and converting the fluorescence into an electrical signal; and an output device for outputting/displaying the electrical signal detected by the PMT. No conversion of the output signal of the output device into image data is needed. A real-time analysis proceeds while samples are being scanned on the biochip. The biochip scanner device of the present invention reads the electrical signal from PMT directly without processing it into image data and setting lens before the PMT is no longer needed. As a result, the structure of the device is simplified and the cost for production is reduced.

Abstract translation: 生物芯片扫描器装置包括用于发射光束的光源; 光处理单元，用于将光束聚焦到生物芯片上以从生物芯片上的荧光目标激发荧光; 用于滤除来自光源的光束的滤光器; 用于检测和转换荧光成电信号的光电倍增管（PMT）; 以及用于输出/显示由PMT检测的电信号的输出装置。 不需要将输出设备的输出信号转换为图像数据。 在生物芯片上扫描样品时，进行实时分析。 本发明的生物芯片扫描器装置在PMT不再需要之前，直接从PMT读取电信号而不将其处理成图像数据和设置透镜。 结果，简化了装置的结构，降低了生产成本。

公开(公告)号：US20080304721A1

公开(公告)日：2008-12-11

申请号：US11760884

申请日：2007-06-11

Applicant: Tzu-Chiang WU ,  Chien-Der Lin

Inventor： Tzu-Chiang WU ,  Chien-Der Lin

IPC: G06K9/00

CPC classification number: G01N21/8483

Abstract: The present invention relates to an image detection method for diagnostic plates, which detects the changes of signal lines in multiple assay regions at the same time, and promotes the detection efficiency remarkably. The diagnostic plates in the present invention may comprise Radio frequency identification (RFID) tags, and comprise at least one assay region, and at least one signal line within the assay region. This image detection method for diagnostic plates according to the present invention comprises: reading the parameters from the RFID tags of the diagnostic plates directly, or reading the alternative RFID tags provided with an RFID reader, or downloading the parameters from external files of computer; detecting the changes of the images whether reaches the stable state; analyzing and measuring the color of signal lines and the background in the image of the diagnostic plates after the images are stabilized; calculating the color intensity difference between the images of the signal line and the corresponding background; and determining the result of the signal lines in the assay region based on the difference in intensity.

Abstract translation: 本发明涉及用于诊断板的图像检测方法，其同时检测多个测定区域中的信号线的变化，并且显着地提高检测效率。 本发明中的诊断板可以包括射频识别（RFID）标签，并且包括至少一个测定区域和测定区域内的至少一条信号线。 根据本发明的用于诊断板的图像检测方法包括：直接从诊断板的RFID标签读取参数，或者读取设置有RFID读取器的备选RFID标签，或者从计算机的外部文件下载参数; 检测图像的变化是否达到稳定状态; 在图像稳定后分析和测量信号线的颜色和诊断板的图像中的背景; 计算信号线的图像与相应背景之间的色强度差; 以及基于强度差确定测定区域中的信号线的结果。

公开(公告)号：US07173701B2

公开(公告)日：2007-02-06

申请号：US10777651

申请日：2004-02-13

Applicant: Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

Inventor： Jiann-Hua Wang ,  Tsung-Kai Chuang ,  Tzu-Chiang Wu ,  Chien-Ho Chuang

IPC: G01J21/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6456

Abstract: A CCD-based biochip reader includes a light source for emitting light beams, a collimating lens for converting the light beams into wide parallel rays of light, passing said wide parallel rays of light through a biochip and exciting fluorescence from fluorescent targets on the biochip, a focusing lens for focusing the fluorescence, a filter for filtering out said parallel rays of light, and a charge-coupled device camera for generating images from said fluorescence. For recording intensity of the fluorescence from the fluorescent targets, images of the charge-coupled device camera is converted into digital data through an image converting device. Wide parallel laser beams are produced by the laser and through the collimating lens, and excite all samples on the biochip with high efficiency at the same time. In addition, time for analysis is saved when fluorescent images of a large area on biochips are collected and analyzed through the charge-coupled device camera.

Abstract translation: 基于CCD的生物芯片读取器包括用于发射光束的光源，用于将光束转换成宽平行光线的准直透镜，使所述宽平行光线通过生物芯片并从生物芯片上的荧光目标激发荧光， 用于聚焦荧光的聚焦透镜，用于滤出所述平行光线的滤光器和用于从所述荧光产生图像的电荷耦合器件相机。 为了记录来自荧光目标的荧光的强度，电荷耦合器件相机的图像通过图像转换装置转换成数字数据。 通过激光和准直透镜产生宽平行激光束，同时以高效率激发生物芯片上的所有样品。 此外，通过电荷耦合器件相机收集和分析生物芯片上大面积的荧光图像时，可节省分析时间。