公开(公告)号：US20140273015A1

公开(公告)日：2014-09-18

申请号：US14124649

申请日：2012-06-06

申请人： Hans-Peter Holthoff ,  Martin Ungerer ,  Stefan Zeibig ,  Martin J. Lohse ,  Roland Jahns ,  Valerie Jahns

发明人： Hans-Peter Holthoff ,  Martin Ungerer ,  Stefan Zeibig ,  Martin J. Lohse ,  Roland Jahns ,  Valerie Jahns

IPC分类号： C07K16/28 ,  G01N33/68 ,  G01N33/74

CPC分类号： G01N33/74 ,  C07K16/286 ,  C07K16/2869 ,  C07K2317/34 ,  C07K2317/92 ,  G01N33/6893 ,  G01N33/9433 ,  G01N2800/325

摘要： The present disclosure relates to binding compounds/antibodies that bind to the second extracellular loop of the human β1-adrenoreceptor (β1-AR-ECII) that are produced by/obtainable from a host cell/hybridoma with a deposit number selected from the group consisting of DSM ACC3121, DSM ACC3174, DSM ACC3175, DSM ACC3176 and DSM AC-C3177. The binding compounds/antibodies are particularly useful in determination of auto-anti-β1-AR antibodies in an in vitro cell based assay system in order to characterize and to identify auto-antibodies directed against the β1-AR-ECII in a biological sample. Further aspects of the disclosure are nucleic acid molecules encoding said binding compounds/antibodies, vectors, host cells, methods for producing the binding compounds/antibodies of the disclosure as well as a kit comprising the binding compounds/antibodies of the present disclosure.

摘要翻译： 本公开涉及结合化合物/抗体，所述化合物/抗体结合由具有选择的保藏号的宿主细胞/杂交瘤产生/可获得的人β-肾上腺素受体（＆bgr; 1-AR-ECII）的第二细胞外环 来自由DSM ACC3121，DSM ACC3174，DSM ACC3175，DSM ACC3176和DSM AC-C3177组成的组。 结合化合物/抗体特别可用于在基于体外​​细胞的测定系统中测定自身抗体和抗体1-AR抗体，以鉴定和鉴定针对1-AR-ECII的自身抗体 生物样品。 本公开的另外方面是编码所述结合化合物/抗体，载体，宿主细胞的核酸分子，用于产生本公开的结合化合物/抗体的方法以及包含本公开内容的结合化合物/抗体的试剂盒。