公开(公告)号：US07312046B2

公开(公告)日：2007-12-25

申请号：US10787015

申请日：2004-02-25

申请人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

发明人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

IPC分类号： C12Q1/02 ,  C12Q1/18 ,  C12Q1/20 ,  C12N1/00 ,  C12N5/00 ,  C12M1/00 ,  C12M3/00

CPC分类号： G01N33/5014 ,  C12M25/14 ,  C12M29/10 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5067 ,  G01N33/5088 ,  G01N33/552 ,  G01N2500/20

摘要： A nanoporous silicon support comprising a plurality of macropores is provided to function as a bioreactor for the maintenance of cells in culture in a differentiated state. Each cell or group of cells is grown in an individual macropore and is provided with nutrients by means such as perfusion of the nanoporous silicon support with fluid. The macropores may be between 0.2 and 200 microns and be coated with a substance that promotes cell adhesion. The support containing cells may be used to used to test compounds for biological activity, metabolism, toxicity, mutagenicity, carcinogenicity or to characterize novel or unknown comounds. The supports are sufficiently robust that they may be assembled into larger reactors to simulate organ function or be used for the production of biomolecules.

摘要翻译： 提供包含多个大孔的纳米多孔硅载体用作生物反应器，用于维持分化状态的培养中的细胞。 每个细胞或一组细胞在单独的大孔中生长，并通过诸如用流体灌注纳米多孔硅载体的方式提供营养。 大孔可以在0.2和200微米之间，并且涂覆有促进细胞粘附的物质。 含有细胞的载体可用于测试化合物的生物活性，代谢，毒性，致突变性，致癌性或表征新颖或未知的化合物。 载体足够坚固，可将其组装成较大的反应器以模拟器官功能或用于生产生物分子。

公开(公告)号：US06734000B2

公开(公告)日：2004-05-11

申请号：US09981691

申请日：2001-10-12

申请人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

发明人： Vicki I. Chin ,  Sangeeta N. Bhatia ,  Michael J. Sailor ,  Boyce E. Collins

IPC分类号： C12M1114

CPC分类号： G01N33/5014 ,  C12M25/14 ,  C12M29/10 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5067 ,  G01N33/5088 ,  G01N33/552 ,  G01N2500/20

摘要： A nanoporous silicon support comprising a plurality of macropores is provided to function as a bioreactor for the maintenance of cells in culture in a differentiated state. Each cell or group of cells is grown in an individual macropore and is provided with nutrients such as by perfusion of the nanoporous silicon support with fluid. The macropores may be between 0.2 and 200 microns and be coated with a substance that provides cell adhesion. The support containing cells may be used to used to test compounds for biological activity, metabolism, toxicity, mutagenicity, carcinogenicity or to characterize novel or unknown comounds. The support is sufficiently robust that it may be assembled into larger reactors to simulate organ function or be used for the production of biomolecules.

摘要翻译： 提供包含多个大孔的纳米多孔硅载体用作生物反应器，用于维持分化状态的培养中的细胞。 每个细胞或一组细胞在单个大孔中生长并且提供营养物质，例如通过用流体灌注纳米多孔硅载体。 大孔可以在0.2至200微米之间，并且涂覆有提供细胞粘附的物质。 含有细胞的载体可用于测试化合物的生物活性，代谢，毒性，致突变性，致癌性或表征新颖或未知的化合物。 支架足够坚固，可将其装配到较大的反应器中以模拟器官功能或用于生产生物分子。