公开(公告)号：US20110224104A1

公开(公告)日：2011-09-15

申请号：US11757020

申请日：2007-06-01

申请人： Evgenia Razumovski ,  Berk Oktem ,  Appavu K. Sundaram ,  Vsevolod S. Rakov ,  Sudeepta Shanbhag ,  Sergey Kurnosenko ,  Vladimir M. Doroshenko

发明人： Evgenia Razumovski ,  Berk Oktem ,  Appavu K. Sundaram ,  Vsevolod S. Rakov ,  Sudeepta Shanbhag ,  Sergey Kurnosenko ,  Vladimir M. Doroshenko

IPC分类号： C40B50/06 ,  C12Q1/04 ,  C12M1/34 ,  C40B60/14

CPC分类号： C12Q1/04 ,  C40B20/08 ,  G01N33/6851 ,  G16B40/00

摘要： A method and system for identification of microorganisms in a sample. The method includes processing the sample to produce at least one group of biomarker molecules from at least one microorganism in the sample, tandem mass-analyzing biomarker fragment ions from the at least one group of biomarker molecules to obtain a sample biomarker tandem mass spectrum of the biomarker, and identifying the microorganism in the sample based on a comparison of the sample biomarker tandem mass spectrum to an experimentally-derived reference tandem mass spectrum (stored in the reference library) from a known microorganism. The system includes a sample processing unit configured to process the sample and produce at least one group of biomarker molecules from at least one microorganism in the sample. The system includes a mass-analyzer for tandem mass analysis biomarker fragment ions. The system includes a processor configured to identify the microorganism in the sample based on a comparison of a sample biomarker tandem mass spectrum to an experimentally-derived reference tandem mass spectrum from a known microorganism.

摘要翻译： 用于鉴定样品中的微生物的方法和系统。 该方法包括处理样品以从样品中的至少一个微生物产生至少一组生物标记分子，来自至少一组生物标志物分子的串联质量分析生物标记片段离子，以获得样品生物标志物串联质谱 基于样品生物标志物串联质谱与来自已知微生物的实验来源的参考串联质谱（存储在参考文库中）的比较来鉴定样品中的微生物。 该系统包括样品处理单元，其被配置为处理样品并从样品中的至少一种微生物产生至少一组生物标记分子。 该系统包括用于串联质量分析生物标志物碎片离子的质量分析器。 该系统包括处理器，其被配置为基于样品生物标志物串联质谱与来自已知微生物的实验来源的参考串联质谱的比较来鉴定样品中的微生物。