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公开(公告)号：US20230265077A1
公开(公告)日：2023-08-24
申请号：US17677565
申请日：2022-02-22
发明人： Xianting Ding , Haiyang Xie , Ting Zhang , Shanhe Li
IPC分类号： C07D403/04 , C07K1/26 , C07D409/04 , C07D405/04 , C07D257/04
CPC分类号： C07D403/04 , C07K1/26 , C07D409/04 , C07D405/04 , C07D257/04
摘要： Provided are a photoactive compound having a chemical structure of formula (I), a photoactive protein-immobilizing gel and use. The photoactive protein-immobilizing gel, in which the photoactive compound is contained or immobilized, can crosslink with a protein under the action of UV light, thereby achieving separation and/or immobilization of the protein.
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公开(公告)号：US20230102204A1
公开(公告)日：2023-03-30
申请号：US17490716
申请日：2021-09-30
发明人： Xianting DING , Ainur ABULA , Ting ZHANG , Haiyang XIE
摘要： A microfluidic chip comprises: a first unit which has a channel for a cell sample to pass through and is configured to separate circulating tumor cells in the cell sample; a second unit, a front end of which communicates with a tail end of the first unit, and the second unit is configured to capture single cells from the separated circulating tumor cells and subject the captured single cells to closed lysis; and a gel layer which is provided at the second unit. The microfluidic chip is configured to implement the binding of a protein molecule of the single cell with an antibody in the gel layer after the single cell is lysed. A cell separation and western blotting method using the microfluidic chip comprises: lysing circulating tumor cells, capturing, and implementing the binding of a lysate with an antibody. A manufacture method of the microfluidic chip, comprises: manufacturing a first interlayer and a separation unit; manufacturing a second interlayer and pasting the second interlayer on a basal layer, and manufacturing a single-cell capture unit; and bonding the first interlayer with the separation unit and the second interlayer with the single-cell capture unit.
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