公开(公告)号：US20180106758A1

公开(公告)日：2018-04-19

申请号：US15707600

申请日：2017-09-18

申请人： ProteinSimple

发明人： Tom W. YANG ,  David John ROACH

IPC分类号： G01N27/447 ,  C07K1/26 ,  C07K1/28

CPC分类号： G01N27/44782 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44791

摘要： An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US09764319B2

公开(公告)日：2017-09-19

申请号：US14123451

申请日：2012-08-01

申请人： Andrea Mahn

发明人： Andrea Mahn

IPC分类号： G01N27/447 ,  B01J39/26 ,  C07K1/26 ,  G01N30/88

CPC分类号： B01J39/26 ,  C07K1/26 ,  G01N27/44756 ,  G01N2030/8831

摘要： The disclosure provides methods and kits for diagnosing the nutritional state of selenium, using six proteins as biomarkers for which the expression increases when the metabolic state is supra-nutritional.

公开(公告)号：US20160025676A1

公开(公告)日：2016-01-28

申请号：US14728471

申请日：2015-06-02

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Thomas Beardslee ,  Timothy Updyke

IPC分类号： G01N27/447

CPC分类号： G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

摘要： Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

摘要翻译： 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。

公开(公告)号：US20160003771A1

公开(公告)日：2016-01-07

申请号：US14768986

申请日：2014-02-19

申请人： THE UNIVERSITY OF QUEENSLAND

发明人： Matt TRAU ,  Muhammad Johirul Alam SHIDDIKY ,  Sakandar RAUF ,  Ramanathan PUDHUKODE VAIDYANATHAN

IPC分类号： G01N27/447 ,  G01N33/483

CPC分类号： G01N27/447 ,  B01L3/50273 ,  B01L2300/0645 ,  B01L2400/0415 ,  C07K1/26 ,  C12Q2565/125 ,  F04B19/006 ,  G01N27/3276 ,  G01N27/44791 ,  G01N33/4836

摘要： A device for the detection of target entities, the device including: a fluidic channel to conduct a liquid sample containing at least one type of target entity to be detected; and mutually spaced electrodes disposed along the fluidic channel; wherein the mutually spaced electrodes are functionalised to selectively bond to the at least one type of target entity and are configured to electro-hydrodynamically pump the liquid sample along the fluidic channel on application of a signal to the electrodes, such that selectivity of attachment of the at least one type of target entity to the electrodes is determined by at least one parameter of the signal.

摘要翻译： 一种用于检测目标实体的装置，该装置包括：流体通道，用于传导含有待检测的至少一种类型的目标实体的液体样品; 以及沿着流体通道设置的相互间隔开的电极; 其中所述相互间隔开的电极被功能化以选择性地结合到所述至少一种类型的目标实体，并且被配置为在向所述电极施加信号时沿着所述流体通道电液体动力地泵送所述液体样品，使得所述液体样品的附着选择性 通过信号的至少一个参数确定至电极的至少一种类型的目标实体。

公开(公告)号：US09164058B2

公开(公告)日：2015-10-20

申请号：US14203351

申请日：2014-03-10

申请人： Bio-Rad Laboratories, Inc.

发明人： Lei Li ,  Xuemei Yang ,  Christopher Belisle

IPC分类号： G01N27/447 ,  C08F2/46 ,  C07K1/26 ,  B65D85/00 ,  B01D57/02 ,  C08K3/00

CPC分类号： G01N27/44747 ,  B01D57/02 ,  B65D85/70 ,  C07K1/26 ,  C08F2/46 ,  C08K3/00 ,  G01N21/6428

摘要： Hand cast gels, and solutions for their preparation, are described.

摘要翻译： 描述了手铸凝胶及其制备方法。

公开(公告)号：US09090666B2

公开(公告)日：2015-07-28

申请号：US13534498

申请日：2012-06-27

申请人： Tiansong Wang

发明人： Tiansong Wang

IPC分类号： F21V7/04 ,  G02B6/00 ,  G02B6/036 ,  G09F13/00 ,  C07K1/26 ,  G01N27/447 ,  G02B6/26

CPC分类号： C07K1/26 ,  G01N27/44721 ,  G02B6/262

摘要： An optical fiber (500) illuminates the bore (520) of a capillary tube (515) that is used for separating chemicals by capillary electrophoresis (CE). The fiber terminates in either two sloped regions (525) and a curved region (530) or two sloped regions (705) and a flat region (700). Light from these regions is focused on the bore of the capillary tube. Since the fiber is sized to illuminate the core of a CE capillary, it is larger than fibers used in telecommunications and its sloped regions are at angles that would be unsuitable for use in telecommunications. The relatively large diameter of the capillary permits efficient use of a light source (905).

摘要翻译： 光纤（500）照射用于通过毛细管电泳（CE）分离化学品的毛细管（515）的孔（520）。 纤维终止于两个倾斜区域（525）和弯曲区域（530）或两个倾斜区域（705）和平坦区域（700）中。 来自这些区域的光聚焦于毛细管的孔。 由于光纤的尺寸可以照亮CE毛细管的核心，因此它大于电信中使用的光纤，其斜坡区域的角度将不适合用于电信。 毛细管的相对大的直径允许有效利用光源（905）。

公开(公告)号：US09057694B2

公开(公告)日：2015-06-16

申请号：US13928137

申请日：2013-06-26

申请人： Life Technologies Corporation

发明人： Timothy Updyke ,  Thomas Beardslee

IPC分类号： G01N27/447 ,  C07K1/26 ,  C08J3/075 ,  C08K5/00 ,  C08L33/26

CPC分类号： G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

摘要： Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

摘要翻译： 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。

公开(公告)号：US20150090591A1

公开(公告)日：2015-04-02

申请号：US14039995

申请日：2013-09-27

申请人： ProteinSimple

发明人： Tom W. Yang ,  David John Roach

IPC分类号： G01N27/447

CPC分类号： G01N27/44782 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44791

摘要： An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

摘要翻译： 一种装置包括限定储存器的主体部分和一组基本上柔性的毛细管。 该组基本上柔性的毛细管固定地连接到主体部分并且与储存器流体连通。 连接器被配置为联接到主体部分以与储存器和一组基本上柔性的毛细管流体连通。 连接器还被配置为耦合到真空源。 该装置被布置成使得主体部分的至少一部分是导电的。 还提供了使用该装置从生物样品分离和检测分析物的方法。 例如，还提供了用于从细胞裂解物或纯化的蛋白质分离和检测一种或多种蛋白质的方法。

公开(公告)号：US08945361B2

公开(公告)日：2015-02-03

申请号：US11524630

申请日：2006-09-20

申请人： Erik Gentalen ,  Daniel J. Suich

发明人： Erik Gentalen ,  Daniel J. Suich

IPC分类号： G01N27/447 ,  C07K1/13 ,  C07K1/26 ,  C07K1/28 ,  C07K7/06 ,  C07K7/08

CPC分类号： G01N27/44726 ,  C07K1/13 ,  C07K1/26 ,  C07K1/28 ,  C07K7/06 ,  C07K7/08 ,  G01N2550/00

摘要： Electrophoresis Compositions, methods and kits useful for, among other things, detecting, quantifying and/or characterizing analytes are provided. The compositions are useful as electrophoresis standards for determine the isoelectric point and molecular weight of an analyte. The electrophoresis standards generally comprise at least one label moiety and one or more reactive moieties that when activated attach the standard to a substrate.

摘要翻译： 提供了用于检测，量化和/或表征分析物的组合物，方法和试剂盒。 该组合物可用作用于测定分析物的等电点和分子量的电泳标准品。 电泳标准品通常包含至少一个标记部分和一个或多个反应性部分，当活化时将标准物附着到基底上。

公开(公告)号：US08945360B2

公开(公告)日：2015-02-03

申请号：US12691440

申请日：2010-01-21

申请人： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

发明人： Shane Petersen ,  Cory Panattoni ,  Craig Rowell

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： G01N27/44747 ,  C07K1/26 ,  Y10S436/826

摘要： Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

摘要翻译： 提供高分辨率的聚丙烯酰胺凝胶作为蛋白质分离的电泳介质，并且通过包含牛磺酸，天冬酰胺或两者作为两性电解质与三（羟甲基） - 氨基甲烷或双（2- 羟乙基）氨基 - 三（羟甲基）甲烷作为缓冲液，加上其他常规组分。