公开(公告)号：US10281473B2

公开(公告)日：2019-05-07

申请号：US15116609

申请日：2015-02-04

申请人： University of Virginia Patent Foundation ,  Donald F. Hunt ,  Weihan Wang ,  Lichao Zhang

发明人： Donald F. Hunt ,  Weihan Wang ,  Lichao Zhang

IPC分类号： G01N33/68

摘要： The application discloses compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8M urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin I, resulting in a product mixture containing 3-10 kDa peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (ETD) and collision activated dissociation mass spectrometry. This method provides 95% sequence coverage of a mAb and detects numerous post-translational modifications. For disulfide bond location, native mAb is subjected to longer digestion times. Release of disulfide containing peptides from accessible regions of the folded protein occurs with short digestion times. The identity of peptides connected by a disulfide bond is determined using ETD and ion-ion proton transfer chemistry.

公开(公告)号：US20160026455A1

公开(公告)日：2016-01-28

申请号：US14773704

申请日：2014-03-07

申请人： Mark Christopher JEFFREY ,  Weihan WANG ,  AIR COMPUTING, INC.

发明人： Mark Christopher JEFFREY ,  Weihan WANG

IPC分类号： G06F9/445 ,  H04L29/08

CPC分类号： G06F8/65 ,  H04L67/1076 ,  H04L67/1095

摘要： In one embodiment, a method for collecting updates for a plurality of objects over a cloud data network includes: determining a set of remote devices known to have updates for a selected object, wherein each of said remote devices maintains a set of locally updated objects that includes the selected object; and downloading the updates for the selected object from said set of remote devices. Where said downloading the updates for the selected object results in a name conflict, the method further includes resolving said name conflict, wherein said resolving includes selecting said selected object as a target and said existing object as an alias having a pointer relationship to the target; and merging all meta-data of the alias object into the target.

摘要翻译： 在一个实施例中，用于通过云数据网络收集多个对象的更新的方法包括：确定已知具有所选对象的更新的一组已知具有更新的远程设备，其中每个所述远程设备维护一组本地更新的对象， 包括所选对象; 以及从所述一组远程设备下载所选对象的更新。 在所述下载所选对象的更新导致名称冲突的情况下，所述方法还包括解析所述名称冲突，其中所述解析包括将所选择的对象作为目标选择，所述现有对象作为具有与目标的指针关系的别名; 并将别名对象的所有元数据合并到目标中。

公开(公告)号：US20120005159A1

公开(公告)日：2012-01-05

申请号：US13176528

申请日：2011-07-05

申请人： Weihan WANG ,  Yuri SAGALOV

发明人： Weihan WANG ,  Yuri SAGALOV

IPC分类号： G06F17/30

CPC分类号： G06F16/1767

摘要： A system and method for cloud file management are disclosed. According to one embodiment, a computer-implemented method comprises registering the first user and the first device with a server, creating a library for object storage, transmitting an invitation to access the library to a second user, the second user having a second device, verifying and granting the second user access to the library, wherein granting the second user access to the library comprises granting the second device access to the library. An object having a replication factor and two or more components is stored on one or more of the first device and the second device according to the replication factor and total storage available on the first device and the second device.

摘要翻译： 公开了一种用于云文件管理的系统和方法。 根据一个实施例，计算机实现的方法包括向服务器注册第一用户和第一设备，创建用于对象存储的库，向第二用户发送访问库的邀请，第二用户具有第二设备， 验证和授予第二用户对库的访问，其中授予第二用户对库的访问包括授予第二设备对库的访问。 具有复制因子和两个或更多个组件的对象根据第一设备和第二设备上可用的复制因子和总存储而存储在第一设备和第二设备的一个或多个上。