公开(公告)号：US07138041B2

公开(公告)日：2006-11-21

申请号：US10784681

申请日：2004-02-23

Applicant: Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

IPC: G01N27/327

CPC classification number: G01N27/3272

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.

Abstract translation: 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。

公开(公告)号：US20050183953A1

公开(公告)日：2005-08-25

申请号：US10784681

申请日：2004-02-23

Applicant: Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

IPC: G01N27/26 ,  G01N33/487

CPC classification number: G01N27/3272

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.

Abstract translation: 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。

公开(公告)号：US20080160543A1

公开(公告)日：2008-07-03

申请号：US11645556

申请日：2006-12-27

Applicant: Chein-Shyong Su ,  Wen-Jing Cheng ,  Maio-Ling Hung ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Wen-Jing Cheng ,  Maio-Ling Hung ,  Tai-Guang Wu

IPC: G01N33/53

CPC classification number: C12Q1/001 ,  G01N33/5438 ,  G01N33/54386

Abstract: An electrochemical immuno diagnostic strip and its preparation method are disclosed herein. A screen-printed electrode is prepared on an insulating substrate, which is divided into an electrode area and a connecting area. An electrochemical precursor is placed on the electrode area and a reaction membrane is provided on the electrode area. A specimen loading area is at the reaction membrane. The present invention is advantageous to overcome the interference problem of the electrochemical detection technology and to provide the quantitative analysis of the prompt diagnostic strip.

Abstract translation: 本文公开了一种电化学免疫诊断条及其制备方法。 在绝缘基板上制备丝网印刷电极，该绝缘基板被分成电极区域和连接区域。 在电极区域上放置电化学前体，在电极区域上设置反应膜。 样品装载区域在反应膜上。 本发明有利于克服电化学检测技术的干扰问题，并提供快速诊断条的定量分析。