公开(公告)号：US07138041B2

公开(公告)日：2006-11-21

申请号：US10784681

申请日：2004-02-23

Applicant: Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

IPC: G01N27/327

CPC classification number: G01N27/3272

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.

Abstract translation: 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。

公开(公告)号：US07749764B2

公开(公告)日：2010-07-06

申请号：US12003564

申请日：2007-12-28

Applicant: Chein-Shyong Su ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Tai-Guang Wu

IPC: G01N33/72

CPC classification number: G01N33/726

Abstract: An electrochemical method and a test strip for detecting hemoglobin in a specimen are provided. The method includes the steps of providing the specimen with a reagent including a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by reaction of the hemoglobin and the electron mediator in the specimen under a potentiostatic condition; and calculating a concentration of the hemoglobin in the specimen according to the detected electric current.

Abstract translation: 提供了用于检测试样中的血红蛋白的电化学方法和测试条。 该方法包括以下步骤：向试样提供包含缓冲溶液，表面活性剂和电子介体的试剂，由环糊精改性的四硫富瓦烯; 在恒电位条件下检测样品中血红蛋白和电子介体反应产生的电流; 以及根据检测到的电流计算样品中血红蛋白的浓度。

公开(公告)号：US20080160543A1

公开(公告)日：2008-07-03

申请号：US11645556

申请日：2006-12-27

Applicant: Chein-Shyong Su ,  Wen-Jing Cheng ,  Maio-Ling Hung ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Wen-Jing Cheng ,  Maio-Ling Hung ,  Tai-Guang Wu

IPC: G01N33/53

CPC classification number: C12Q1/001 ,  G01N33/5438 ,  G01N33/54386

Abstract: An electrochemical immuno diagnostic strip and its preparation method are disclosed herein. A screen-printed electrode is prepared on an insulating substrate, which is divided into an electrode area and a connecting area. An electrochemical precursor is placed on the electrode area and a reaction membrane is provided on the electrode area. A specimen loading area is at the reaction membrane. The present invention is advantageous to overcome the interference problem of the electrochemical detection technology and to provide the quantitative analysis of the prompt diagnostic strip.

Abstract translation: 本文公开了一种电化学免疫诊断条及其制备方法。 在绝缘基板上制备丝网印刷电极，该绝缘基板被分成电极区域和连接区域。 在电极区域上放置电化学前体，在电极区域上设置反应膜。 样品装载区域在反应膜上。 本发明有利于克服电化学检测技术的干扰问题，并提供快速诊断条的定量分析。

公开(公告)号：US5629202A

公开(公告)日：1997-05-13

申请号：US467523

申请日：1995-06-06

Applicant: Chein-Shyong Su ,  Zen-Jen Chan ,  Wuen-Hsian Huang ,  Hsin Tsai

Inventor： Chein-Shyong Su ,  Zen-Jen Chan ,  Wuen-Hsian Huang ,  Hsin Tsai

IPC: C12M1/36 ,  C12P13/22

CPC classification number: C12M41/48 ,  C12P13/22

Abstract: Disclosed is an automatic computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan which comprises a bioreactor linked to a computer equipped with an on-line indole assay device, in which the computer controls the feeding of indole, pyruvic acid and/or its salt and an ammonium salt into the bioreactor and feedback-controls the reaction in the bioreactor by indole concentration determined by the on-line indole assay device, wherein the feeding of indole and ammonium salt is controlled by a pre-defined profile that agrees with a tryptophanase activity profile and pyruvic acid and/or its salt is added at a predetermined time.

Abstract translation: 公开了一种用于L-色氨酸的酶合成的自动计算机控制的生物反应器系统，其包括连接到装备有在线吲哚测定装置的计算机的生物反应器，其中计算机控制吲哚，丙酮酸和/或其 盐和铵盐进入生物反应器，并通过在线吲哚测定装置确定的吲哚浓度反馈控制生物反应器中的反应，其中吲哚和铵盐的进料由预定义的分布控制，其与 在预定时间加入色氨酸酶活性谱和丙酮酸和/或其盐。

公开(公告)号：US20080057525A1

公开(公告)日：2008-03-06

申请号：US11515881

申请日：2006-09-06

Applicant: Chein-Shyong Su ,  Hung Miao-Ling ,  Ning-Jun Jing ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Hung Miao-Ling ,  Ning-Jun Jing ,  Tai-Guang Wu

IPC: C12Q1/60

CPC classification number: C12Q1/60 ,  C12Q1/005

Abstract: A sensing member for detecting total cholesterol of a blood sample is disclosed, which includes: a working electrode and a counter electrode respectively formed on a first substrate and a second substrate, wherein, an opening is formed in one of the first and second substrates corresponding in position to the electrode for allowing the blood sample to pass through; a dielectric layer formed on one of the surfaces of the first and second substrates and exposing the electrode and end connector such that the end connector can be electrically connected to an electronic device used for applying electric voltage; a reactive pad disposed between the working electrode and the counter electrode and containing an reactive reagent, wherein the reactive reagent comprises an electron transport material, an enzyme capable of reacting with the analyte and generating a current corresponding to the concentration, and a buffer solution; and a separating pad disposed on the opening for removing interfering materials from the blood sample before the blood sample enters into the opening. By disposing the separating pad at a specified position, corpuscles and interfering giant molecules can be removed from the blood sample so as to quickly and accurately detect total cholesterol of the whole blood sample.

Abstract translation: 公开了一种用于检测血液样本的总胆固醇的感测部件，其包括：分别形成在第一基板和第二基板上的工作电极和对置电极，其中，开口形成在第一和第二基板中的一个对应物 位于电极上以允许血液样品通过; 形成在所述第一和第二基板的一个表面上的电介质层，并且暴露所述电极和端部连接器，使得所述端部连接器可以电连接到用于施加电压的电子装置; 设置在工作电极和对电极之间并含有反应性试剂的反应性焊盘，其中反应试剂包括电子传输材料，能够与分析物反应并产生对应于浓度的电流的酶和缓冲溶液; 以及设置在开口上的分离垫，用于在血液样本进入开口之前从血液样品中除去干扰物质。 通过将分离垫设置在指定位置，可以从血液样品中除去小体和干扰性的巨型分子，以便快速准确地检测全血样品的总胆固醇。

公开(公告)号：US20050183953A1

公开(公告)日：2005-08-25

申请号：US10784681

申请日：2004-02-23

Applicant: Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Chia-Wei Chang ,  Miao-Ling Hung ,  Wen-Jing Cheng ,  Tai-Guang Wu

IPC: G01N27/26 ,  G01N33/487

CPC classification number: G01N27/3272

Abstract: An electrochemical biosensor formed by screen printing and method of fabricating such biosensor is disclosed in the present invention. The biosensor can quickly absorb a sample to be measured therein, effectively control volume of the sample fed and “fill-and-position” the sample therein. The biosensor includes an electrode layer (electrode area) comprising two or three electrodes, which are a working electrode, a reference electrode and an auxiliary electrode (tri-electrode) on an insulating substrate. An active reaction layer containing reactant, reaction catalyst, mediator, wetting agent and surfactant is spread on the surface of the electrode layer. A sample inflow area is formed above the electrode area by adding an upper cover on top of a middle insulating layer with a U-shaped opening formed therein. Sample solution with a minute amount about 0.8 to 1 μl can be rapidly introduced into the electrode area and the active reaction layer via the inflow area by siphon or capillary, where the ingredient of the sample can be analysed by measuring reaction between the sample, reaction catalyst and mediator in the reaction layer using electrochemical potentiometric or amperometric method. An upwardly extended closed space formed within the upper cover above the electrode area adjacent to the front of conductive wires can be effectively used to control sample volume and “fill-and-position” the sample.

Abstract translation: 在本发明中公开了通过丝网印刷形成的电化学生物传感器和制造这种生物传感器的方法。 生物传感器可以快速吸收要测量的样品，有效地控制样品进料的体积并将样品“填充并定位”。 生物传感器包括在绝缘基板上包括作为工作电极，参考电极和辅助电极（三电极）的两个或三个电极的电极层（电极区域）。 含有反应物，反应催化剂，介体，润湿剂和表面活性剂的活性反应层扩散在电极层的表面上。 通过在其中形成有U形开口的中间绝缘层的顶部添加上盖，在电极区域的上方形成样品流入区域。 通过虹吸或毛细管可以通过虹吸或毛细管通过流入区域快速地将约0.8至1微摩尔的微量的样品溶液引入电极区域和活性反应层，其中样品的成分可以通过测量样品，反应 催化剂和介质在反应层中使用电化学电位或电流法。 可以有效地使用形成在电极区域之上的上电极区域内的向上延伸的封闭空间，该电极区域与导电线的前部相邻，以控制样品体积和“填充和定位”样品。

公开(公告)号：US4970164A

公开(公告)日：1990-11-13

申请号：US334199

申请日：1989-04-06

Applicant: Chin-Ping Yang ,  Chein-Shyong Su

Inventor： Chin-Ping Yang ,  Chein-Shyong Su

IPC: C08B37/16 ,  C12P19/04 ,  C12P19/14

CPC classification number: C12P19/04 ,  C08B37/0012 ,  C12P19/14

Abstract: A method of recovering and separating water-soluble cyclodextrins from cyclodextrin formation liquid, wherein the CD formation liquid or the CD liquid after separating .beta.-CD by concentrated precipitation method is mixed with .alpha.-amylase to hydrolyze dextrins to small oligosaccharides, then is concentrated and precipitated again to separate the remained .beta.-CD, after that, soluble .alpha.- and .gamma.-CD are recovered by using inclusion extraction method with organic solvent from the residual liquid.

Abstract translation: 从环糊精形成液中回收分离水溶性环糊精的方法，其中通过浓缩沉淀法将β-CD分离后的CD形成液体或CD液体与α-淀粉酶混合以将糊精水解成小寡糖，然后浓缩， 再沉淀分离剩余的β-CD，然后通过使用夹杂物萃取方法从残留液体中回收有机溶剂中的可溶性α和γ-CD。

公开(公告)号：US20090104641A1

公开(公告)日：2009-04-23

申请号：US12003564

申请日：2007-12-28

Applicant: Chein-Shyong Su ,  Tai-Guang Wu

Inventor： Chein-Shyong Su ,  Tai-Guang Wu

IPC: C12Q1/20 ,  G01N33/72

CPC classification number: G01N33/726

Abstract: The present invention provides an electrochemical method and a test strip for detecting hemoglobin in a specimen. The method includes the steps of providing the specimen with a reagent, which has a buffer solution, a surfactant and an electron mediator, tetrathiafulvalene, modified by cyclodextrin; detecting electric current produced by the reaction of the hemoglobin and the electron mediator in a specimen under a potentiostatic condition; and calculating a hemoglobin concentration in the specimen according to the detected electric current. In the method of the present invention, tetrathiafulvalene modified by cyclodextrin is used as an electron mediator, which directly interacts with the hemoglobin in the specimen. Since the hemoglobin concentration is in inverse proportion to the value of electric current or the value of SOC (state-of-charge) integrated by electric current value with time, the hemoglobin concentration in a whole blood specimen or the hematocrit level in a blood specimen is accurately and rapidly obtained according to the detected electric value or the value of SOC.

Abstract translation: 本发明提供一种用于检测样品中的血红蛋白的电化学方法和测试条。 该方法包括以下步骤：向样品提供具有缓冲溶液，表面活性剂和电子介体的试剂，由环糊精改性的四硫富瓦烯; 在恒电位条件下检测样品中血红蛋白和电子介体的反应产生的电流; 并根据检测到的电流计算样品中的血红蛋白浓度。 在本发明的方法中，将通过环糊精改性的四硫富瓦烯用作电子介体，其直接与样品中的血红蛋白相互作用。 由于血红蛋白浓度与电流值或通过电流值随时间积分的SOC（充电状态）的值成反比，所以血样中的血红蛋白浓度或血样中的血细胞比容水平 根据检测到的电值或SOC的值准确而快速地获得。

公开(公告)号：US5279946A

公开(公告)日：1994-01-18

申请号：US727696

申请日：1991-07-10

Applicant: Chein-Shyong Su ,  Huey-Lin Chung ,  Wuen-Hsian Huang ,  Hsin Tsai

Inventor： Chein-Shyong Su ,  Huey-Lin Chung ,  Wuen-Hsian Huang ,  Hsin Tsai

IPC: C12P21/02 ,  C07K5/072 ,  C12N9/50

CPC classification number: C07K5/0613 ,  C12N9/50

Abstract: Disclosed is a process for the preparation of N-benzyloxycarbonyl-.alpha.-L-aspartyl-L-phenylalanine methyl ester wherein a protease is used to catalyze the condensation reaction of N-benzyloxycarbonyl-L-aspartic acid with L-phenylalanine methyl ester in a system consisting of ethyl acetate, citrate buffer with the addition of glycerol as a dispersing agent.

Abstract translation: 公开了制备N-苄氧羰基-α-L-天冬氨酰基-L-苯丙氨酸甲酯的方法，其中使用蛋白酶催化N-苄氧羰基-L-天冬氨酸与L-苯丙氨酸甲酯的缩合反应 系统由乙酸乙酯，柠檬酸盐缓冲液组成，加入甘油作为分散剂。