公开(公告)号：US5714474A

公开(公告)日：1998-02-03

申请号：US626554

申请日：1996-04-02

申请人： Albert J. J. Van Ooijen ,  Krijn Rietveld ,  Andreas Hoekema ,  Jan Pen ,  Peter Christian Sijmons ,  Teunis Cornelis Verwoerd ,  Wilhemus Johannes Quax

发明人： Albert J. J. Van Ooijen ,  Krijn Rietveld ,  Andreas Hoekema ,  Jan Pen ,  Peter Christian Sijmons ,  Teunis Cornelis Verwoerd ,  Wilhemus Johannes Quax

IPC分类号： A23K1/165 ,  C12N9/16 ,  C12N9/28 ,  C12N15/82 ,  A61K48/00 ,  A01H5/10 ,  A61K38/43

CPC分类号： C12Y301/03026 ,  A23K20/189 ,  C12N15/8242 ,  C12N15/8243 ,  C12N15/8257 ,  C12N9/16 ,  C12N9/2417

摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.