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公开(公告)号：US6022846A
公开(公告)日：2000-02-08
申请号：US97847
申请日：1998-06-15
申请人： Albert J. J. Van Ooijen , Krun Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
发明人： Albert J. J. Van Ooijen , Krun Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
摘要翻译： 本发明提供了植酸酶在转基因植物或植物器官中的表达及其生产方法。 提供DNA表达构建体用于在能够指导植酸酶表达的调控序列的控制下用编码植酸酶的基因转化植物。 这些调节序列包括根据植物或其部分的用途能够在植物中指导转录的序列，组成型或阶段和/或组织特异性的序列。 本发明提供的转基因植物和植物器官可以直接应用于各种工业过程，例如， 在动物饲料中，或者，可以提取表达的肌醇六磷酸酶，如果需要，在施用前进行纯化。
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公开(公告)号：US5714474A
公开(公告)日：1998-02-03
申请号：US626554
申请日：1996-04-02
申请人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd , Wilhemus Johannes Quax
发明人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd , Wilhemus Johannes Quax
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds to increase the rate of reaction. By directly adding the seeds to the reaction mixture the method provides a solution to the expensive and problematic process of extracting and purifying the enzyme. Methods of treatment are also provided whereby a subject lacking a sufficient supply of an enzyme is administered the enzyme in the form of seeds containing enhanced amounts of the enzyme.
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公开(公告)号：US5770413A
公开(公告)日：1998-06-23
申请号：US693709
申请日：1996-08-07
申请人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
发明人： Albert J. J. Van Ooijen , Krijn Rietveld , Andreas Hoekema , Jan Pen , Peter Christian Sijmons , Teunis Cornelis Verwoerd
IPC分类号： A01H1/00 , A01H5/00 , A23K1/14 , A23K1/16 , A23K1/165 , C12N1/21 , C12N5/10 , C12N9/16 , C12N9/28 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/82 , C12P3/00 , C12P7/02 , C12P7/18 , C12R1/01 , C12R1/66
CPC分类号： C12Y301/03026 , A23K20/189 , C12N15/8242 , C12N15/8243 , C12N15/8257 , C12N9/16 , C12N9/2417
摘要： The present invention provides for the expression of phytase in transgenic plants or plant organs and methods for the production of such plants. DNA expression constructs are provided for the transformation of plants with a gene encoding phytase under the control of regulatory sequences which are capable of directing the expression of phytase. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or stage and/or tissue specific, depending on the use of the plant or parts thereof. The transgenic plants and plant organs provided by the present invention may be applied to a variety of industrial processes either directly, e.g. in animal feeds or alternatively, the expressed phytase may be extracted and if desired, purified before application.
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