公开(公告)号：US5834252A

公开(公告)日：1998-11-10

申请号：US425684

申请日：1995-04-18

申请人： Willem Peter Christiaan Stemmer ,  Robert J. Lipshutz

发明人： Willem Peter Christiaan Stemmer ,  Robert J. Lipshutz

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  C12N9/14

CPC分类号： C12Q1/6853 ,  C12N15/10

摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof and for assembling large polynucleotides from component polynucleotides, each involving generating concatemers formed by PCR amplification of overlapping fragments.

公开(公告)号：US5733731A

公开(公告)日：1998-03-31

申请号：US548540

申请日：1995-10-26

申请人： Peter J. Schatz ,  Millard G. Cull ,  Jeff F. Miller ,  Willem Peter Christiaan Stemmer ,  Christian M. Gates

发明人： Peter J. Schatz ,  Millard G. Cull ,  Jeff F. Miller ,  Willem Peter Christiaan Stemmer ,  Christian M. Gates

IPC分类号： C12N1/21 ,  C12N15/10 ,  C12Q1/68 ,  C12N15/72

CPC分类号： C12N15/1075

摘要： A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.

摘要翻译： 通过用编码由DNA结合蛋白和随机肽组成的融合蛋白的重组载体的集合转化宿主细胞构建的随机肽文库，并且编码DNA结合蛋白的结合位点可用于筛选新配体。 筛选方法导致通过DNA结合蛋白形成包含通过随机肽配体与受体结合的融合蛋白和重组DNA载体的复合物。

公开(公告)号：US6156511A

公开(公告)日：2000-12-05

申请号：US10216

申请日：1998-01-21

申请人： Peter J. Schatz ,  Millard G. Cull ,  Jeff F. Miller ,  Willem Peter Christiaan Stemmer ,  Christian M. Gates

发明人： Peter J. Schatz ,  Millard G. Cull ,  Jeff F. Miller ,  Willem Peter Christiaan Stemmer ,  Christian M. Gates

IPC分类号： C12N1/21 ,  C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1075

摘要： A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA. binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.

摘要翻译： 通过用编码由DNA结合蛋白和随机肽组成的融合蛋白的重组载体的集合转化宿主细胞构建的随机肽文库，并编码DNA的结合位点。 结合蛋白可用于筛选新配体。 筛选方法导致通过DNA结合蛋白形成包含通过随机肽配体与受体结合的融合蛋白和重组DNA载体的复合物。