公开(公告)号：US20120196927A1

公开(公告)日：2012-08-02

申请号：US13296356

申请日：2011-11-15

Applicant: Young-Sil Lee ,  Byung-Yoon Cha ,  Xiao-Xing Wang ,  Sun-Sil Choi ,  Bong-Keun Choi ,  Hiroshi Yamakawa ,  Kiyoto Saito ,  Takayuki Yonezawa ,  Toshiaki Teruya ,  Kazuo Nagai ,  Je-Tae Woo

Inventor： Young-Sil Lee ,  Byung-Yoon Cha ,  Xiao-Xing Wang ,  Sun-Sil Choi ,  Bong-Keun Choi ,  Hiroshi Yamakawa ,  Kiyoto Saito ,  Takayuki Yonezawa ,  Toshiaki Teruya ,  Kazuo Nagai ,  Je-Tae Woo

IPC: A61K31/353 ,  C12Q1/68 ,  G01N21/64 ,  A61P3/00

CPC classification number: A61K31/352 ,  A23L33/105 ,  A23L33/30 ,  A23V2002/00 ,  A61K36/752 ,  C12Q1/6883 ,  C12Q2600/136 ,  G01N2500/00 ,  A23V2200/328 ,  A23V2200/332 ,  A23V2250/20

Abstract: A method for screening a substance for treating visceral adiposity syndrome comprises: (1) extracting a total RNA fraction from skeletal muscle collected from non-human mammals of a group to which a predetermined amount of the test substance has been administered and from non-human mammals of a group to which the test substance has not been administered; (2) quantifying the expression quantity of mRNA in the total RNA fraction extracted that encodes a glucose transporter gene by a specific method; and (3) comparing and analyzing the quantity of the test substance ingested, the body weight gain, and the expression quantity of RNA encoding the glucose transporter gene during the administration period for the non-human mammals of the test substance-administered group to these quantities for the non-human mammals that constitute the test substance-unadministered group to thereby determine the effect of the test substance on the metabolic syndrome.

Abstract translation: 用于筛选用于治疗内脏肥胖症综合征的物质的方法包括：（1）从已经给予预定量的测试物质的组的非人哺乳动物收集的骨骼肌中提取总RNA级分，并从非人类 被检物质未经施用的群体的哺乳动物; （2）通过具体方法定量提取的编码葡萄糖转运蛋白基因的总RNA级分中mRNA的表达量; 和（3）比较和分析所测试物质给药组的非人哺乳动物在给药期间所摄入的测试物质的数量，体重增加和编码葡萄糖转运蛋白基因的RNA的表达量 构成被检物质的非人类哺乳动物的数量 - 未施用组，从而确定测试物质对代谢综合征的影响。