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1.发明授权Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids 有权用于错配修复缺陷的宿主细胞及其在使用单链核酸诱导同源重组的方法中的用途公开(公告)号：US07521242B2
公开(公告)日：2009-04-21
申请号：US10841125
申请日：2004-05-07
申请人： Donald L. Court , Xin-tian Li , Jian-Dong Huang , Nina Costantino , Depei Liu
发明人： Donald L. Court , Xin-tian Li , Jian-Dong Huang , Nina Costantino , Depei Liu
摘要： Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
摘要翻译： 本文公开了使用单链核酸分子在包含靶核酸的宿主细胞中诱导同源重组的方法。 单链核酸分子具有与目标核酸同源的足够数量的核苷酸，以实现与靶核酸的同源重组。 宿主细胞包括可操作地连接到编码单链结合蛋白的核酸并且缺陷以用于错配修复的去阻抑性启动子。 还公开了在该方法中使用的分离的宿主细胞。
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2.发明申请Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids 有权用于错配修复缺陷的宿主细胞及其在使用单链核酸诱导同源重组的方法中的用途公开(公告)号：US20050079618A1
公开(公告)日：2005-04-14
申请号：US10841125
申请日：2004-05-07
申请人： Donald Court , Xin-tian Li , Jian-Dong Huang , Nina Costantino , Depei Liu
发明人： Donald Court , Xin-tian Li , Jian-Dong Huang , Nina Costantino , Depei Liu
摘要： Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
摘要翻译： 本文公开了使用单链核酸分子在包含靶核酸的宿主细胞中诱导同源重组的方法。 单链核酸分子具有与目标核酸同源的足够数量的核苷酸，以实现与靶核酸的同源重组。 宿主细胞包括可操作地连接到编码单链结合蛋白的核酸并且缺陷以用于错配修复的去阻抑性启动子。 还公开了在该方法中使用的分离的宿主细胞。
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3.发明授权公开(公告)号：US06847311B2
公开(公告)日：2005-01-25
申请号：US10108407
申请日：2002-03-28
申请人： Xin-Tian Li
发明人： Xin-Tian Li
IPC分类号： G06F17/21 , G06F3/023 , G06F17/22 , H03M11/04 , H03M11/08 , H04M1/02 , H04M1/23 , H04M1/247 , H04M1/725 , H03K17/94 , H03M11/00
CPC分类号： H04M1/72519 , G06F3/0233 , H04M2250/70
摘要： A method and apparatus for character entry and more particularly Chinese character entry. The method improves the ease of entry for Chinese characters on a device that has a keypad arrangement of at least a 3×3 matrix (106). For example wireless communication devices such as radiotelephones have a keypad (108) for dialing phone numbers. Arranging the Chinese characters on the device display in a 3×3 matrix to match the at least 3×3 matrix of the keypad allows the user to quickly select from the pin yin list (104) the key on the keypad that corresponds to the desired character in the matching matrix.
摘要翻译： 用于字符输入的方法和装置，更具体地说是汉字输入。 该方法提高了具有至少3×3矩阵（106）的键盘布置的设备上的汉字的输入的容易性。 例如，诸如无线电话机的无线通信设备具有用于拨打电话号码的小键盘（108）。 将3x3矩阵中的汉字排列在3x3矩阵中，以匹配键盘的至少3x3矩阵，用户可以从引脚列表（104）快速选择键盘上与匹配中所需字符相对应的键 矩阵。
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