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1.发明授权Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids 有权用于错配修复缺陷的宿主细胞及其在使用单链核酸诱导同源重组的方法中的用途公开(公告)号：US07521242B2
公开(公告)日：2009-04-21
申请号：US10841125
申请日：2004-05-07
申请人： Donald L. Court , Xin-tian Li , Jian-Dong Huang , Nina Costantino , Depei Liu
发明人： Donald L. Court , Xin-tian Li , Jian-Dong Huang , Nina Costantino , Depei Liu
摘要： Methods are disclosed herein for inducing homologous recombination in a host cell comprising a target nucleic acid, using a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule has a sufficient number of nucleotides homologous to the target nucleic acid to enable homologous recombination with the target nucleic acid. The host cell includes a de-repressible promoter operably linked to a nucleic acid encoding a single-stranded binding protein and is deficient for mismatch repair. Isolated host cells of use in this method are also disclosed.
摘要翻译： 本文公开了使用单链核酸分子在包含靶核酸的宿主细胞中诱导同源重组的方法。 单链核酸分子具有与目标核酸同源的足够数量的核苷酸，以实现与靶核酸的同源重组。 宿主细胞包括可操作地连接到编码单链结合蛋白的核酸并且缺陷以用于错配修复的去阻抑性启动子。 还公开了在该方法中使用的分离的宿主细胞。
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2.发明申请PLASMIDS AND PHAGES FOR HOMOLOGOUS RECOMBINATION AND METHODS OF USE 有权用于同种异体重建的PLASMIDS和PHAGES及其使用方法公开(公告)号：US20100136692A1
公开(公告)日：2010-06-03
申请号：US12688764
申请日：2010-01-15
申请人： Donald L. Court , Simanti Datta , Nina Costantino
发明人： Donald L. Court , Simanti Datta , Nina Costantino
CPC分类号： C12N15/74 , C12N7/00 , C12N15/70 , C12N15/73 , C12N2795/10321 , C12N2795/10343
摘要： Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.
摘要翻译： 公开了可用于将重组功能引入宿主细胞的λ噬菌体。 还公开了可用于向多种大肠杆菌菌株和其它细菌（包括沙门氏菌，假单胞菌，蓝细菌，螺旋体）赋予重组功能的质粒。 这些质粒和噬菌体可以在体外分离，并可用于转化细菌细胞，如革兰氏阴性细菌。
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公开(公告)号：US07674621B2
公开(公告)日：2010-03-09
申请号：US11134795
申请日：2005-05-20
申请人： Donald L. Court , Simanti Datta , Nina Costantino
发明人： Donald L. Court , Simanti Datta , Nina Costantino
CPC分类号： C12N15/74 , C12N7/00 , C12N15/70 , C12N15/73 , C12N2795/10321 , C12N2795/10343
摘要： Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.
摘要翻译： 公开了可用于将重组功能引入宿主细胞的λ噬菌体。 还公开了可用于向多种大肠杆菌菌株和其它细菌（包括沙门氏菌，假单胞菌，蓝细菌，螺旋体）赋予重组功能的质粒。 这些质粒和噬菌体可以在体外分离，并可用于转化细菌细胞，如革兰氏阴性细菌。
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4.发明申请ENHANCED HOMOLOGOUS RECOMBINATION MEDIATED BY LAMBDA RECOMBINATION PROTEINS 审中-公开由LAMBDA重组蛋白介导的增强的同源重组公开(公告)号：US20100267093A1
公开(公告)日：2010-10-21
申请号：US12760461
申请日：2010-04-14
申请人： Donald L. Court , Daiguan Yu , E-Chiang Lee , Hilary M. Ellis , Nancy A. Jenkins , Pentao Liu , Neal G. Copeland
发明人： Donald L. Court , Daiguan Yu , E-Chiang Lee , Hilary M. Ellis , Nancy A. Jenkins , Pentao Liu , Neal G. Copeland
IPC分类号： C12N15/87
CPC分类号： A01K67/0275 , A01K2217/05 , C12N15/66 , C12N15/8213 , C12N15/902 , C12N2800/204 , C12N2800/30 , C12N2830/002 , C12N2830/55 , C12N2830/60 , C12N2840/203
摘要： Disclosed herein are methods for generating recombinant DNA molecules in cells using homologous recombination mediated by recombinases and similar proteins. The methods promote high efficiency homologous recombination in bacterial cells, and in eukaryotic cells such as mammalian cells. The methods are useful for cloning, the generation of transgenic and knockout animals, and gene replacement. The methods are also useful for subcloning large DNA fragments without the need for restriction enzymes. The methods are also useful for repairing single or multiple base mutations to wild type or creating specific mutations in the genome. Also disclosed are bacterial strains and vectors which are useful for high-efficiency homologous recombination.
摘要翻译： 本文公开了使用由重组酶和类似蛋白质介导的同源重组在细胞中产生重组DNA分子的方法。 这些方法促进细菌细胞和真核细胞如哺乳动物细胞中的高效同源重组。 该方法可用于克隆，转基因和敲除动物的产生以及基因替换。 该方法也可用于亚克隆大的DNA片段而不需要限制酶。 该方法对修复单基因或多基因突变至野生型或在基因组中产生特异性突变也是有用的。 还公开了可用于高效同源重组的细菌菌株和载体。
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公开(公告)号：US07144734B2
公开(公告)日：2006-12-05
申请号：US10366044
申请日：2003-02-12
申请人： Donald L. Court , Daiguan Yu , E-Chiang Lee , Hilary M. Ellis , Nancy A. Jenkins , Neal G. Copeland
发明人： Donald L. Court , Daiguan Yu , E-Chiang Lee , Hilary M. Ellis , Nancy A. Jenkins , Neal G. Copeland
CPC分类号： A01K67/0275 , A01K2217/05 , C12N15/66 , C12N15/8213 , C12N15/902 , C12N2800/204 , C12N2800/30 , C12N2830/002 , C12N2830/55 , C12N2830/60 , C12N2840/203
摘要： Disclosed herein are methods for generating recombinant DNA molecules in cells using homologous recombination mediated by recombinases and similar proteins. The methods promote high efficiency homologous recombination in bacterial cells, and in eukaryotic cells such as mammalian cells. The methods are useful for cloning, the generation of transgenic and knockout animals, and gene replacement. The methods are also useful for subcloning large DNA fragments without the need for restriction enzymes. The methods are also useful for repairing single or multiple base mutations to wild type or creating specific mutations in the genome. Also disclosed are bacterial strains and vectors which are useful for high-efficiency homologous recombination.
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公开(公告)号：US08541229B2
公开(公告)日：2013-09-24
申请号：US12688764
申请日：2010-01-15
申请人： Donald L. Court , Costantino Nina
发明人： Donald L. Court , Costantino Nina
CPC分类号： C12N15/74 , C12N7/00 , C12N15/70 , C12N15/73 , C12N2795/10321 , C12N2795/10343
摘要： Lambda phages that can be used to introduce recombineering functions into host cells are disclosed. Also disclosed are plasmids that can be used to confer recombineering functions to a variety of strains of E. coli and to other bacteria, including Salmonella, Pseudomonas, Cyanobacteria, Spirochaetes. These plasmids and phages can be isolated in vitro and can be used to transform bacterial cells, such as gram negative bacteria.
摘要翻译： 公开了可用于将重组功能引入宿主细胞的λ噬菌体。 还公开了可用于向多种大肠杆菌菌株和其它细菌（包括沙门氏菌，假单胞菌，蓝细菌，螺旋体）赋予重组功能的质粒。 这些质粒和噬菌体可以在体外分离，并可用于转化细菌细胞，如革兰氏阴性细菌。
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7.发明授权Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era 失效筛选延迟细胞周期的试剂的方法和包含时代和野生型时代的类似物的组合物
公开(公告)号：US6132954A
公开(公告)日：2000-10-17
申请号：US915498
申请日：1997-08-20
摘要： The present invention provides for methods of screening for agents which delay the cell cycle and methods of delaying the cell cycle. Analogues of Era having arginine, histidine, or lysine at amino acid codon 17 are embodied by the present invention. Human and other homologs of bacterial Era amino acid and nucleic acid sequences are provided in the present invention. Vectors, host cells, protein preparations, cell cultures, and compositions comprising said analogue are also set forth in the present invention.
摘要翻译： 本发明提供筛选延迟细胞周期的试剂的方法和延迟细胞周期的方法。 具有氨基酸密码子17的精氨酸，组氨酸或赖氨酸的时代的类似物由本发明体现。 在本发明中提供了人和其他同源物的细菌时代氨基酸和核酸序列。 载体，宿主细胞，蛋白质制剂，细胞培养物和包含所述类似物的组合物也在本发明中阐述。
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