公开(公告)号：US20100092432A1

公开(公告)日：2010-04-15

申请号：US12310034

申请日：2007-08-07

申请人： Takenori Ozaki ,  Kaoru Yasuda ,  Shouichi Maruyama ,  Tokunori Yamamoto ,  Momokazu Gotoh ,  Seiichi Matsuo ,  Yasuo Kitagawa

发明人： Takenori Ozaki ,  Kaoru Yasuda ,  Shouichi Maruyama ,  Tokunori Yamamoto ,  Momokazu Gotoh ,  Seiichi Matsuo ,  Yasuo Kitagawa

IPC分类号： A61K35/12 ,  A61P9/10 ,  A61P13/12 ,  A61P13/02 ,  A61P17/02

CPC分类号： C12N5/0667 ,  A61K35/12

摘要： [Problems] To provide a novel use of multipotential stem cells originating in a fat tissue.[Means for Solving Problems] It is intended to provide a cell preparation which contains multipotential stem cells originating in a fat tissue and is usable for an ischemic disease, impairment of renal function, wound, urinary incontinence or osteoporosis. As the multipotential stem cells originating in a fat tissue, use is made of cells which proliferate in the case of centrifuging cells separated from a fat tissue and culturing the thus sedimented cells (an SVF fraction) under low-serum conditions. In an embodiment, a cell preparation containing the SVF fraction is provided.

摘要翻译： [问题]提供源自脂肪组织的多潜能干细胞的新用途。 解决问题的手段旨在提供一种包含源于脂肪组织的多能干细胞并可用于缺血性疾病，肾功能损害，伤口，尿失禁或骨质疏松症的细胞制剂。 作为源于脂肪组织的多能干细胞，使用在离心从脂肪组织分离的细胞并在低血清条件下培养沉淀的细胞（SVF级分）的情况下增殖的细胞。 在一个实施方案中，提供了含有SVF级分的细胞制剂。

公开(公告)号：US20070202592A1

公开(公告)日：2007-08-30

申请号：US11631818

申请日：2005-07-08

申请人： Yasuo Kitagawa ,  Shuhei Torii ,  Kazuhiro Sakurada ,  Masato Kobori ,  Yuzuru Kamei ,  Hirotake Yamaguchi ,  Kazuhiro Toriyama ,  Toru Takada

发明人： Yasuo Kitagawa ,  Shuhei Torii ,  Kazuhiro Sakurada ,  Masato Kobori ,  Yuzuru Kamei ,  Hirotake Yamaguchi ,  Kazuhiro Toriyama ,  Toru Takada

IPC分类号： C12N5/06 ,  C12N5/08

CPC分类号： C12N5/0667

摘要： It is intended to provide a pluripotent cell having the following properties: (1) being contained in a mixed-cell type population obtained by enzymatic treatment of the tissue collected from an animal; (2) being contained in a sedimented cell population obtained by centrifugating the mixed-cell type population of the property (1); (3) selectively proliferating by culturing in a medium containing 2% (v/v) or lower serum and 1 to 100 ng/ml of fibroblast growth factor-2; and (4) being differentiated into cells having the characteristics of adipocytes, osteoblasts, chondrocytes, tendon cells, myocardial cells, myoblasts, neurocytes or vascular endothelial cells by adjusting the culture condition; cells having differentiated from the above cell; and a method of conveniently obtaining the pluripotent cell in a large amount.

摘要翻译： 旨在提供具有以下性质的多能细胞：（1）包含在通过酶处理从动物收集的组织获得的混合细胞型群体中; （2）包含在通过离心所述性质的混合细胞型群体（1）获得的沉降细胞群体中; （3）通过在含有2％（v / v）或更低血清和1至100ng / ml成纤维细胞生长因子-2的培养基中培养而选择性增殖; （4）通过调节培养条件将细胞分化为具有脂肪细胞，成骨细胞，软骨细胞，肌腱细胞，心肌细胞，成肌细胞，神经细胞或血管内皮细胞特征的细胞; 与上述细胞有区别的细胞; 以及大量方便地获得多能细胞的方法。