公开(公告)号：US20240301357A1

公开(公告)日：2024-09-12

申请号：US18272815

申请日：2022-01-17

Applicant: UNIVERSITE DE GENEVE ,  LES HOPITAUX UNIVERSITAIRES DE GENEVE

Inventor： SANAE EL-HARANE ,  OLIVIER PREYNAT-SEAUVE ,  KARL-HEINZ KRAUSE ,  STÉPHANE DURUAL ,  ALI MODARRESSI ,  THOMAS BRASCHLER

IPC: C12N5/0775 ,  C12N5/074 ,  C12N5/0797

CPC classification number: C12N5/0667 ,  C12N5/0623 ,  C12N5/0696 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2535/10 ,  C12N2539/00

Abstract: The present invention is directed to a substrate material for the formation of cell spheroids/organoids and uses thereof. The invention further relates to methods of preparation of such a substrate material and related tools and uses thereof, methods using such a substrate material for the formation of cell spheroids and resulting spheroids and uses thereof.

公开(公告)号：US20240271069A1

公开(公告)日：2024-08-15

申请号：US18620076

申请日：2024-03-28

Applicant: YOUTH BIO GLOBAL CO., LTD.

Inventor： Seung Ho YOO ,  Chi Young CHANG

IPC: C12M1/32 ,  C12M1/34 ,  C12M1/36 ,  C12M1/42 ,  C12N5/0775

CPC classification number: C12M23/12 ,  C12M35/02 ,  C12M41/46 ,  C12M41/48 ,  C12N5/0667

Abstract: A device for forming a chondroprogenitor cell aggregate according to the present embodiment comprises: a plurality of wells in each of which a micromass is disposed; an electrical stimulation unit which provides electrical stimulation to the micromasses; an image acquisition unit which acquires images by photographing, at predetermined time intervals, the micromasses disposed in the plurality of wells; and a stimulation provision determination unit which determines from the images whether to provide electrical stimulation, wherein the stimulation provision determination unit determines whether to provide the electrical stimulation from a degree of aggregation of the micromass provided with the electrical stimulation.

公开(公告)号：US12059438B2

公开(公告)日：2024-08-13

申请号：US17448684

申请日：2021-09-23

Applicant: QINGDAO HAIER BIOTECH CO., LTD

Inventor： Qilong Cao ,  Fei Wang ,  Hui Li ,  Guohu Di

IPC: A61K31/00 ,  A61K9/00 ,  A61K35/28 ,  A61K47/18 ,  A61K47/24 ,  A61K47/36 ,  C12N5/0775

CPC classification number: A61K35/28 ,  A61K9/0048 ,  A61K47/186 ,  A61K47/24 ,  A61K47/36 ,  C12N5/0667 ,  C12N2509/00

Abstract: The present disclosure relates to an eyedrop applicable to limbal stem cell deficiency and a preparation method, wherein each liter of the eyedrop includes the following components: 1 to 40 mg of human adipose-derived stem cell exosomes, excipients: 0.5 to 2 g of sodium hyaluronate, 0.5 to 3 g of vitamin B6 and 0.05 to 0.3 g of benzalkonium chloride, and the balance of medical normal saline, a pH of the eyedrop is 6.5 to 7.5; and the eyedrop of the present disclosure is effective in treating limbal stem cell deficiency.

公开(公告)号：US20240228970A1

公开(公告)日：2024-07-11

申请号：US18417480

申请日：2024-01-19

Applicant: Prolific Machines Inc.

Inventor： Deniz KENT ,  Maximiliaan HUISMAN ,  Declan JONES ,  Victor JONES ,  Monique HOOLEY ,  Emily GALE ,  William C. PATON

IPC: C12N5/0775 ,  C12M1/00

CPC classification number: C12N5/0662 ,  C12M31/00 ,  C12N5/0667 ,  C12N2501/115 ,  C12N2510/00 ,  C12N2529/10

Abstract: Provided herein are methods and systems for growing and maintaining stem cells in suspension. In some cases, the methods and systems involve the use of optical switches for activating one or more growth factor signaling pathways involved in the growth and/or maintenance of stem cells.

公开(公告)号：US12018244B2

公开(公告)日：2024-06-25

申请号：US18094446

申请日：2023-01-09

Applicant: DSM IP Assets B.V.

Inventor： John E. Nash ,  William T. Fisher ,  Gino Bradica

IPC: C12M1/00 ,  B04B3/00 ,  B04B7/12 ,  C12N5/0775

CPC classification number: C12M47/04 ,  C12M47/02 ,  B04B3/00 ,  B04B7/12 ,  C12N5/0667

Abstract: A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

公开(公告)号：US12018243B2

公开(公告)日：2024-06-25

申请号：US17088322

申请日：2020-11-03

Applicant: The Duke Limited

Inventor： Marcos Sforza

IPC: C12M1/00 ,  A61K35/35 ,  C12M1/12 ,  C12M1/33 ,  C12N5/077 ,  C12N5/0775

CPC classification number: C12M47/04 ,  A61K35/35 ,  C12M25/02 ,  C12M45/02 ,  C12N5/0653 ,  C12N5/0667 ,  C12N2527/00

Abstract: The present invention provides a device for adipose tissue processing, microfragmentation and facilitation of mechanical separation of adipose derived stem cells (“ADSCs”), methods of using the device to generate a stromal vascular fraction (“SVF”) and methods of the SVF.

公开(公告)号：US12005048B2

公开(公告)日：2024-06-11

申请号：US17404276

申请日：2021-08-17

Applicant: Michael A. Scarpone

Inventor： Michael A. Scarpone

IPC: A61K31/436 ,  A61K35/35 ,  A61L27/36 ,  A61M1/00 ,  C12M1/00 ,  C12M1/26 ,  C12N5/077 ,  C12N5/0775

CPC classification number: A61K31/436 ,  A61K35/35 ,  A61L27/3633 ,  A61M1/63 ,  A61M1/71 ,  A61M1/892 ,  C12M33/10 ,  C12M47/04 ,  C12M47/08 ,  C12N5/0653 ,  C12N5/0667 ,  A61M1/895 ,  A61M2202/0014 ,  A61M2202/0021 ,  A61M2202/095 ,  A61M2205/3331 ,  A61M2205/3365

Abstract: Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

公开(公告)号：US11959104B2

公开(公告)日：2024-04-16

申请号：US17712785

申请日：2022-04-04

Applicant: MEMORIAL SLOAN-KETTERING CANCER CENTER

Inventor： Lorenz Studer ,  Bastian Zimmer ,  Jason Tchieu

IPC: C12N5/00 ,  C12N5/0735 ,  C12N5/074 ,  C12N5/0775 ,  C12N5/079 ,  C12N5/0793 ,  A61K35/12

CPC classification number: C12N5/0667 ,  C12N5/0018 ,  C12N5/0606 ,  C12N5/0618 ,  C12N5/0619 ,  C12N5/0696 ,  A61K35/12 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/04 ,  C12N2510/00 ,  C12N2533/52

Abstract: The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into neural crest, cranial placode or non-neuro ectoderm precursors, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating neurodegenerative and pituitary disorders.

公开(公告)号：US11883438B2

公开(公告)日：2024-01-30

申请号：US16327817

申请日：2017-08-23

Applicant: National University Corporation Nagoya University ,  Gifu University ,  Gifu Prefecture

Inventor： Tokunori Yamamoto ,  Satoshi Suzuki ,  Yasuhito Funahashi ,  Yoshihisa Matsukawa ,  Momokazu Gotoh ,  Tomohiro Kitayama ,  Yoichiro Hoshino ,  Tetsuma Murase

IPC: A61K35/50 ,  A61K35/51 ,  A61K35/52 ,  A61P15/08 ,  A61K35/32 ,  A61K35/54 ,  A61K35/28 ,  A61K35/35 ,  A61D19/00 ,  A61D19/02 ,  A61K35/00 ,  C12N5/076 ,  C12N5/0775

CPC classification number: A61K35/50 ,  A61D19/00 ,  A61D19/02 ,  A61K35/00 ,  A61K35/28 ,  A61K35/32 ,  A61K35/35 ,  A61K35/51 ,  A61K35/52 ,  A61K35/54 ,  A61P15/08 ,  C12N5/061 ,  C12N5/0664 ,  C12N5/0665 ,  C12N5/0667 ,  C12N2500/80

Abstract: It is an object to provide a technique useful for embryo transfer and development of reproductive medical care. Provided are a sperm activator containing a disrupted product of one or more cells selected from the group consisting of adipose tissue-derived stem cells, dental pulp-derived stem cells, bone marrow-derived stem cells, and umbilical cord blood-derived stem cells as an active ingredient, and an artificial insemination method utilizing the same.

公开(公告)号：US11879138B2

公开(公告)日：2024-01-23

申请号：US17434117

申请日：2020-06-29

Applicant: CK-EXOGENE CO., LTD.

Inventor： Jae Young Kim ,  Yong Joon Chwae

IPC: C12N5/0775 ,  A61K8/14 ,  A61K35/28

CPC classification number: C12N5/0667 ,  A61K8/14 ,  A61K35/28 ,  A61K2800/10

Abstract: The present application provides a method for preparing mesenchymal stem cell-derived exosomes, the method including: obtaining a cell sample from mesenchymal stem cells and sub-culturing the cell sample, culturing the sub-cultured cells in a substrate medium containing a protein synthesis inhibitory enzyme, and then obtaining a cell culture solution, and isolating exosomes from the cell culture solution, and a cell culture solution produced therefrom. The method for preparing exosomes of the present application has an advantage in that high purity and high concentration exosomes can be isolated.